Enhanced pancreatic and skin tumorigenesis in cabbage-fed hamsters and mice

Abstract

Studies were conducted to evaluate the ability of dietary dried cabbage supplements to inhibit pancreatic carcinogenesis in hamsters and skin tumorigenesis in mice. Pancreatic cancer was induced by treatment with 40 mg/kg body wt N-nitrosobis-(2-oxopropyl)amine (BOP). Cabbage was fed from before carcinogen treatment in low fat diet and, beginning 1 week after BOP treatment, cabbage was given in low fat and high fat diets in comparison with the respective non-cabbage-containing diets. Dried cabbage was incorporated at 9 and 11% levels into the low and high fat diets. Feeding cabbage in the high fat diet elevated the yield of BOP-induced pancreatic ductular carcinoma (1.6 carcinomas/effective animal) in comparison with that observed in hamsters fed cabbage in a low fat diet or in those given a high fat diet without cabbage, 0.6-0.8 carcinomas/effective animal (P less than 0.05). Furthermore, the incidence of BOP-induced gall bladder adenocarcinoma was elevated in cabbage-fed hamsters irrespective of dietary fat intake. Effects of dietary fat and cabbage on food consumption, body weight, and serum T3 and T4 values are described. Skin tumorigenesis was induced in SENCAR mice by 10 nmol 7,12 dimethylbenz[a]anthracene (DMBA) and promoted beginning 1 week later with twice weekly applications of 2 micrograms 12-O-tetradecanoyl-13-phorbol acetate (TPA). Dried cabbage was incorporated into AIN semi-purified diets from before DMBA treatment and throughout TPA treatment. Skin papilloma yield was elevated in DMBA-initiated TPA-promoted mice that were fed diets containing 10% cabbage. Mice fed cabbage developed an average of 8.45 papillomas per mouse following 22 weeks of promotion while mice given control diet developed 7.25 papillomas per mouse (P less than 0.001). Cabbage feeding did not influence survival, food consumption or body weight of the mice. These results suggest the need for further research on the use of cabbage as a chemopreventive measure.

Glucosinolates and Glucosinolate Derivatives: Implications for Protection Against Chemical Carcinogenesis

“…Diets with 10 % dried cabbage, for instance, have been shown to increase the incidence of pancreatic ductular carcinomas induced by N-nitroso-bis(2-oxopropy1)amine (Birt et al 1987) in mice. A study carried out by Srisangnam ef af.…”

Chemopreventive Effects of Cabbage on 7,12-Dimethylbenz(a)-Anthracene-Induced Hepatocarcinogenesis in Toads (Bufo viridis).

“…On the other hand, cabbage exerted a slightly enhancing effect on the incidence of pancreatic tumors in the hamster model and on skin tumor formation in a mouse model (12). According to the large number of chemical components that must be present in cabbage, it would not expected to completely block tumorigenicity in the toad liver.…”

“…On the contrary, cabbage-fed hamsters and mice exhibited an elevation in pancreas cancer and skin papilloma formation after administration of N nitroso bis-(2-oxy-propyl)-amine and DMBA, respectively (12 liver tumors in 12 of 100 cases (12%). Group De DBA cabbage diet (2ml), 3h after the carcinogen.…”

Medicinal and Edible Plants as Cancer Preventive Agents

“…D i f f e r e n t t y p e s o f l e a v e s , depending on location and season, have been part of the human diet since prehistoric times. Birt, et al, 1987 Skin-1 Inhibition of DMBA/ 12-O-tetradecanoylphorbol-13-acetate (TPA) induced skin tumors Slaga, et al, 1980 Skin-2 Inhibition of benzo[a]pyrene/croton oil induced skin tumors Sadhana, et al, 1988 Skin-3 Inhibition of DMBA/ teleocidin induced skin tumors Yoshizawa, et al, 1984 Skin-4 Inhibition of DMBA/ TPA+mezerein induced skin tumors Perchellet, et al, 1990 Skin-5 Inhibition of DMBA/ ultra violet B (UVB) induced skin tumors Kapadia, et al, 2003 Skin-6 Inhibition of DMBA/fumonisin B1 induced skin tumors Takasaki, et al,1999a Skin-7 Inhibition of (+)-(E)-4-methyl 2[(E)-hydroxyimino]-5-nitro-6-methoxy-3-hexenamido (NOR-1)/TPA induced skin tumors Konoshima, et al, 1999 Tongue Inhibition of 4-NQO induced tongue tumors Tanaka, et al, 1992 Uterus Inhibition of MNU/estradiol-17 induced endometrial tumor Niwa, et al, 2001 Table 1. Bioassay systems related to cancer chemopreventive activities described in this chapter.…”

“…In European folk medicine, cabbage leaves are used to treat acute inflammation, and fresh cabbage juice has been shown to promote rapid healing of peptic ulcers. Oral administration of cabbage inhibited N-nitrosobis-(2-oxopropyl)amine (BOP)-induced pancreatic carcinogenesis in hamsters; however cabbage was not observed to inhibit tumor promotion by DMBA/TPA in www.intechopen.com mouse skin (Birt, et al, 1987). Oral intake of cabbage suppressed mammary carcinogenesis by N-methyl-N-nitrosourea (MNU) in rat (Bresnick, et al, 1990).…”