Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent TolerantBacillus tequilensisRG-01: Thermostable and Surfactant Resistant

Abstract

Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca2+ ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

“…The enzyme retained above 45% of residual activity after longer period of incubation. The finding of the present study strongly agrees with the report of Tiwari et al (2014) who also observed sustainability of crude amylase from Bacillus sp. up to 65 °C.…”

“…The supplementation of other carbon sources revealed the reduced amylase yield under SSF. The utilization of soluble starch for the production of reasonable amount of extracellular amylase was previously reported by Tiwari et al (2014) and Khusro and Aarti (2015). In contrary to this, Narang and Satyanarayana (2001), and Vijayaraghavan et al (2015) demonstrated the increased amylase yield in the presence of lactose and fructose, respectively, from respective bacterial sources.…”

“…On the other hand, Tiwari et al (2014) reported amylases from B. tequilensis with stability towards higher concentration of Hg 2+ . The extraordinary resistant of amylase to various organic solvent revealed another crucial characteristic of strain ALA4.…”

Optimization of Bacteria Amylase Activity from Bacillus licheniformis Strain SEM11

“…The nutritional composition of wheat, potatoes, and sago has nutrients smaller than rice. Optimum activity of bacteria amylase from Bacillus tequilensis RG-01 was obtained by wheat (Tiwari et al, 2014). In contrast to Bacillus licheniformis optimum activity of bacteria amylase obtained by using starch as a substrate (Divakaran et al, 2011).…”

Isolation of Thermophilic Bacteria and Optimizing the Medium Growth Conditions

“…Production and activity of amylase is increase using tapioca flour as carbon source on medium growth of Bacillus sp (Sreekanth et al, 2013). On the Bacillus tequilensis RG-01, amylase production was increased using wheat bran as carbon source (Tiwari et al, 2014). It is different on Bacillus subtilis BI19 where it was using of rice flour as carbon source can stimulate amylase production.…”