Optimization of Bacteria Amylase Activity from  Bacillus licheniformis Strain SEM11

Megahati, Ruth Rize Paas; Urdin, M.; Agustien, Anthoni; Tjong, Djong Hon

doi:10.20546/ijcmas.2017.611.345

Cited by 8 publications

(6 citation statements)

References 5 publications

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“…In this study, twenty-nine bacterial isolates were screened for hydrolytic enzymes, amylase, cellulase and protease enzymes. Various extracellular enzymes from thermophilic bacteria isolated from hot springs have also reported by Al-awsy et al (2017), Megahati et al(2017) and Alrumman et al(2018). Twenty-two isolates produced at least one extracellular enzyme at different high temperature.…”

Section: Discussionsupporting

confidence: 56%

Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Taha

El-Shatoury

Tolba

et al. 2020

African Journal of Biological Sciences

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This study aims to isolate and identify thermophilic hydrolytic bacteria from hot spring in South Sinai, Egypt for several industrial applications. In this work, 29 bacterial isolates from hot spring in Egypt were isolated and screened for the production of three thermozymes (amylase, cellulase and protease) at different high temperatures. Fifteen isolates were amylase producer, twenty-one were cellulase producer and twelve isolates were protease producer at different high temperatures. Ten bacterial isolates (34.5%) produced the three extracellular enzymes. All bacterial isolates were identified phenotypically as Bacillus spp. This study concludes that hot springs in Egypt is a source for the isolation of thermophilic bacteria producing thermostable enzymes.

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Section: Discussionsupporting

confidence: 56%

Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Taha

El-Shatoury

Tolba

et al. 2020

African Journal of Biological Sciences

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“…The activity of the enzyme was influenced by reaction conditions as temperature, pH, substrate concentration, etc. Among several parameters, we only chose those three parameters to optimize amylase activity as they are considered very crucial for the activity of the enzyme, and they are mostly selected for optimizing enzyme activity by other resear chers (Megahati et al, 2017;Dutta et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Comparative Characterization and Amylase Activity Assessment of Certain Garden Bacterial Isolates

Islam¹,

Zerin²

2019

Microb. Bioact.

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Background: Microorganisms are the most important sources of enzymes because of their stability and reduced price in production. The enzyme, amylase has a wide spectrum of application in biotechnology including food, fermentation, textile and laundry, paper and pulp industries. As the use of amylase is increased, it is necessary to search for a new source to produce amylase with better productivity in continuous practice. Method: In our present investigation, amylase producing bacteria were screened by starch hydrolysis test as bacteria are more potent in amylase production than other microorganisms. The bacteria were identified by Bergey's manual of systematic bacteriology. Enzyme assay and optimization of enzyme activity were performed by the 3,5-dinitrosalicylic acid method. Results: A total of eight bacterial isolates were identified with starch degrading capabilities and they were presumptively placed in the genus, Bacillus due to their characteristic features. All the isolates have potential for amylase activity. At any temperature (25˚C, 35˚C, 45˚C, 50˚C and 55˚C), pH (6, 7, 8) and starch concentrations (0.5%, 1% and 1.5%), the highest amylase activity was observed by isolate 1A followed by isolate 2C, 1B, 3A, 4B, 3B, 4A, and 4C. Optimum conditions for the highest amylase activity in our laboratory for isolate 1A was 35˚C (4.105 U/ml), pH 6 (3.343 U/ml) and 1.5% starch concentration (4.381 U/ml). Conclusion: Our study reveals that the isolates, collected from garden soil, are good amylase producers and they could be exploited in different industries in optimized conditions.

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“…Amylase produced by bacteria or bacteria amylase is an enzyme that can hydrolyze starch to sugar (Megahati et al 2017). Amylase can convert starch to sugar in bioethanol production.…”

Section: Introductionmentioning

confidence: 99%

Thermozyme Amylase from Enterobacter sp Extremophiles in Bioethanol Production

Indriati¹,

Megahati

2021

JoSSEd

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Bioethanol is an alternative fuel to replace fossil fuels due to the reduction of fossil fuels. Bioethanol is produced from starch and converted to sugar by thermozyme amylase. Thermozyme amylase produced by thermophile bacteria can be obtained from Pariangan hot springs which have a temperature of 55 °C, pH of 9.2, and have a high level of bacterial diversity. The aim of this study was to obtained isolates of thermozyme amylase-producing bacteria that have the potential for bioethanol production. The research method were bacteria isolation, amylase stability test, temperature and pH optimization, and bioethanol production. The result showed that Enterobacter sp has been isolated from Pariangan hot springs is stable up to 5 hours of incubation, temperature and pH optimum was 85 °C, pH 8.5, and fructose as a carbon source. Thermozyme amylase converts starch into sugar under optimum conditions with a yield of 9.8% bioethanol

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Optimization of Bacteria Amylase Activity from Bacillus licheniformis Strain SEM11

Cited by 8 publications

References 5 publications

Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Comparative Characterization and Amylase Activity Assessment of Certain Garden Bacterial Isolates

Thermozyme Amylase from Enterobacter sp Extremophiles in Bioethanol Production

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