Enhanced production of plumbagin in immobilized cells of Plumbago rosea by elicitation and in situ adsorption

Palle, Komaraiah; Ramakrishna, S. V.; Reddanna, Pallu; Kishor, P. B. Kavi

doi:10.1016/s0168-1656(02)00338-3

Cited by 100 publications

(49 citation statements)

References 20 publications

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“…Chitosan at 100 mg/l gave the highest content of plumbagin in the shoot (1.5 ± 0.1 mg/g dry wt) while plumbagin in the root (7 ± 0.2 mg/g dry wt) reached its maximum after adding 50 mg chitosan/l for 6 days. This is similar to a previous report on plumbagin production in immobilized cells of Plumbago rosea using chitosan treatment (Komaraiah et al 2003). Figure 4 shows that salicylic acid at 100 lM significantly stimulated plumbagin production regardless of treatment duration.…”

Section: Resultssupporting

confidence: 91%

Enhanced plumbagin production from in vitro cultures of Drosera burmanii using elicitation

et al. 2010

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Section: Resultssupporting

confidence: 91%

Enhanced plumbagin production from in vitro cultures of Drosera burmanii using elicitation

et al. 2010

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“…Such effects on the retardation of cell growth have been reported in cultures of Impatiens balsamina L. (Balsaminaceae) treated with methyl jasmonate (Sakunphueak & Panichayupakaranant, 2010). Furthermore, it has been reported that the in situ extraction and elicitation of cell treatment had a synergistic effect on the stimulation of plumbagin production in suspension cultures of P. rosea (Komaraiah et al, 2003).…”

Section: Resultsmentioning

confidence: 79%

“…The main drawback of the cultured plant cells is often lower yields, stability of the cell lines, inconsistency in the production and the storage of the metabolites within the cells or vacuoles (Komaraiah et al, 2003). The 20-Gy-treated root cultures were therefore subcultured to observe the stability of growth and plumbagin production in a three generation set of subcultures.…”

Section: Resultsmentioning

confidence: 99%

Increased production of plumbagin inPlumbago indicaroot cultures by gamma ray irradiation

Jaisi¹,

Sakunphueak

Panichayupakaranant

2013

Pharmaceutical Biology

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Context: Plumbagin is a major active constituent of Plumbago indica L. (Plumbaginaceae). It possesses various pharmacological activities that have been shown to assist in the treatment of various diseases. Objectives: This work is focused on increasing the production of plumbagin in P. indica root cultures using low doses of gamma ray irradiation as an elicitor. Materials and methods: The effect of low doses of gamma ray irradiation (0, 5, 10, 15, 20, 25 Gy) and ages of the root cultures (0, 5, 10, 15, 20 days) for elicitation of plumbagin production was determined. The stability of the elicited root cultures to produce plumbagin was also determined during three cycles of subculture. Results and discussion: Treatment of the root cultures with a low dose of gamma ray at 20 Gy gave the highest level of plumbagin production (1.04 mg/g DW) when compared to all other treated groups. The appropriate age of the root cultures for maximum production of plumbagin was found to be 10 days. However, treatment of 5-day-old root cultures resulted in a significant increase of dried root biomass that also had a high plumbagin production. Based on the total biomass per culture flask, the amounts of plumbagin produced by the 5-and 10-day-old treated roots were 0.59 and 0.37 mg/250 mL flask, respectively, which were 4.2-and 2.6-fold higher than the level in the control. Subculturing the root cultures until the third generation still showed an increase in plumbagin production without any effects on their growth.

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“…An improving of rhinacanthin production in the root cultures was therefore examined by mimic immobilization technique. Immobilization technique with calcium alginate gel has successfully increased naphthoquinone production in Plumbago rosea (Komaraiah et al, 2003). In this study the cultured roots were transferred into the MS medium containing 4 g/L agar and maintained as semisolid culture on the shaker (80 rmp).…”

Section: Rhinacanthin Production In R Nasutus Root Culturesmentioning

confidence: 99%

Effect of medium composition and light on root and rhinacanthin formation inRhinacanthus nasutuscultures

Panichayupakaranant¹,

Meerungrueang²

2010

Pharmaceutical Biology

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Enhanced production of plumbagin in immobilized cells of Plumbago rosea by elicitation and in situ adsorption

Cited by 100 publications

References 20 publications

Enhanced plumbagin production from in vitro cultures of Drosera burmanii using elicitation

Enhanced plumbagin production from in vitro cultures of Drosera burmanii using elicitation

Increased production of plumbagin inPlumbago indicaroot cultures by gamma ray irradiation

Effect of medium composition and light on root and rhinacanthin formation inRhinacanthus nasutuscultures

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