Enhanced prolidase activity and decreased collagen content in breast cancer tissue

Abstract

Adherent interactions between integrins and extracellular matrix (ECM) proteins play an important role in tumorigenicity and invasiveness. The major component of ECM is collagen that plays a central role in the interaction with integrins. The expression of certain collagenases (gelatinases) by tumour cells is one of the characteristic features of the so-called metastatic phenotype, presumably by breaking down ECM barriers as well by altering the ECM-cell interaction. Although extracellular collagenases initiate the breakdown of collagen, the final step of collagen degradation is catalysed by intracellular prolidase. Collagen deposition, gelatinolytic and prolidase activities, expression of beta(1)-integrin receptor and their possible relationships were studied in seven operable breast cancer cases. In breast cancer tissue, we have found significant decrease in the amount of collagen. The decrease in collagen deposition in breast cancer tissue was accompanied by increase in the tissue gelatinolytic and prolidase activities. Simultaneously, a slight decrease in the expression of beta(1)-integrin receptor in breast cancer tissue was observed. These results suggest that alteration in collagen metabolism in breast cancer tissue may reflect tissue remodelling, characteristic for invasive phenotype of cancer cells. Increased gelatinolytic and prolidase activities in breast cancer tissue may enhance stromal matrix degradation and thus may promote metastatic dissemination. On the basis of the data, it seems that compounds endowed with gelatinolytic and prolidase inhibitory activities may be considered as a potential drug candidates for breast cancer therapy.

Effects of Polymeric Nanoparticle Surface Properties on Interaction With Brain Tumor Environment

“…31–33 as well as a decrease in collagen content and deposition within other tumor tissues based on tissue type as well as age. 30,34,35 These results confirm that collagen content varies significantly between tumor tissue types, as well as within the stage of tumor development within a singular tissue type.…”

“…Brain and prostate tissues were chosen to emphasize the significant collagen content variations between tissue types, as tissue such as breast tumors have been associated with decreased collagen content through tumor progression. 30 Analysis showed that collagen content changes with tumor progression dependent on tissue type. Brain tissue demonstrated an increase in collagen content as the tumor developed from Grade 1 to Grade 3, showing little collagen content at Grade 1.…”

Plasma Metabolomic Profiles of Breast Cancer Patients after Short-term Limonene Intervention

“…Collagen is a major component of the extracellular matrix that serves as connective tissue that fills the interstitial space between cells [43]. Breast cancer tissues have been reported to have a significant decrease in collagen and an accompanying increase in collagen degradative enzyme activity [44]. Conversely, percent breast density is the strongest known, non-familial breast cancer risk factor, with the dense portion being primarily composed of collagen [45].…”

Serum Prolidase Activity and Oxidative Status in Patients With Stage I Endometrial Cancer

“…6 The central event in the progression of cancer is the secretion of MMPs, which are involved in matrix remodeling and cell growth. 7 Among the MMPs, prolidase is a manganese-dependent cytosolic exopeptidase, and it cleaves imidodipeptides and imidotripeptides with C-terminal proline or hydroxyproline. 8 It is the obligate enzyme, and its activity has been established in plasma, erythrocytes, leukocytes, and dermal fibroblasts, as well as in various organs including the kidney, brain, heart, thymus, liver, and uterus.…”