Enhanced sensitivity and responses to viologens from a whole‐cell bacterial bioreporter treated with branched polyethyleneimines

Mabekou, Sandrine Soh; Lee, Sang Cheon; Dinh, Thu Huong; Won, Keehoon; Mitchell, Robert J.

doi:10.1111/jam.13592

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…We next evaluated if the toxicities of the combustion and acid-leached NPs stem from the production oxidative radicals, , which may lead to DNA damage . To explore this, three bacterial bioreporter strains were selected as each responds sensitively to specific stressors (Table S3), that is, DNA-damage, , hydrogen peroxide, or the superoxide radical. , As shown in Figures S1–S3, none of the silica NP preparations induce a response from any of these bacterial strains. Based on the sensitivity profiles for each of the bioreporter strains in the literature, − we can reliably conclude that the quantities of reactive oxygen species these NPs produce are insignificant.…”

Section: Resultsmentioning

confidence: 99%

Alkali Extraction to Detoxify Rice Husk-Derived Silica and Increase Its Biocompatibility

Park

Mun

Chun

et al. 2022

ACS Sustainable Chem. Eng.

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As interest in natural biogenic silica nanoparticles (NPs) from rice husks grows, it is important to know how their preparation (i.e., combustion, acid leaching, or alkali extraction) impacts their biocompatibility. Acid leaching and alkali extraction both generated highly pure silica NPs (>99.1% SiO2 vs 93.1% with combustion). However, toxicity tests with six different human and mouse cell lines found alkali-extracted silica NPs were the least harmful; for example, the concentration leading to 50% loss in viability (LC50) was typically around 500, 500–2000, and >2000 mg/L for the combustion, acid-leached, and alkali-extracted silica NPs, respectively. To mimic the presence of carbon during calcination (900 °C for 6 h), polyethylene glycol (PEG) was incorporated in the alkali-extracted silica NPs prior to this step. This significantly increased their toxicities; that is, the LC50 dropped to between 500 and 2000 mg/L and was exacerbated further when calcination was performed at a lower temperature and time (550 °C for 2 h): LC50 values dropped to between 62.5 to 1000 mg/L. These results show that the biocompatibility of rice husk-derived silica NPs is negatively affected by the presence of residual carbon during calcination, but also that it can be significantly improved via alkali extraction.

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Section: Resultsmentioning

confidence: 99%

Alkali Extraction to Detoxify Rice Husk-Derived Silica and Increase Its Biocompatibility

Park

Mun

Chun

et al. 2022

ACS Sustainable Chem. Eng.

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Enhanced microbial fuel cell (MFC) power outputs through Membrane Permeabilization using a branched polyethyleneimine

Soh

Lee

Mitchell

2020

Biosensors and Bioelectronics

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Quantitative Chemical Sensing Using Genetically Engineered Bacterial Bioreporters

Uziel,

Kabessa,

Shemer

et al. 2024

Chemosensors

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We present a generic quantitative chemical sensing methodology for assessing the concentration of a target material (TM) in an aqueous solution by using bioluminescent microbial bioreporters as the core sensing elements. Such bioreporters, genetically engineered to respond to the presence of a TM in their microenvironment by emitting bioluminescence, have previously been mostly designed to report the presence or absence of the TM in the sample. We extend this methodology to also assess the TM concentration, by exploiting the dose-dependency of the TM-induced luminescence. To overcome luminescence intensity variations due to bacterial batch differences and the ambient temperature, simultaneous measurements were carried out on sample solutions containing known concentrations of the TM. A “standard ratio” parameter, defined as the ratio between the two measurements, is shown to be independent of the bacterial batch and the temperature, and hence provides the conceptual basis for a generic quantitative chemical sensing methodology. Assessment of 2,4-dinitrotoluene (DNT) concentration in solutions is demonstrated with an accuracy of 2.5% over a wide dynamic range.

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Enhanced sensitivity and responses to viologens from a whole‐cell bacterial bioreporter treated with branched polyethyleneimines

Abstract: Improving bacterial membrane permeability in a controlled manner using BPEIs can improve biosensing of toxic compounds, as well as be used in biofuel and bioenergy applications where membrane permeability to a solute is important.

Cited by 3 publications

References 53 publications

Alkali Extraction to Detoxify Rice Husk-Derived Silica and Increase Its Biocompatibility

Alkali Extraction to Detoxify Rice Husk-Derived Silica and Increase Its Biocompatibility

Enhanced microbial fuel cell (MFC) power outputs through Membrane Permeabilization using a branched polyethyleneimine

Quantitative Chemical Sensing Using Genetically Engineered Bacterial Bioreporters

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