Enhancement in Seed Priming-Induced Starch Degradation of Rice Seed Under Chilling Stress via GA-Mediated α-Amylase Expression

Song, Shaokun; Yin, Qi; Zhao, Tingcheng; Liu, Hongyan; He, Aibin; Wang, Weiqin

doi:10.1186/s12284-022-00567-3

Cited by 24 publications

(12 citation statements)

References 66 publications

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“…Activated α-amylase initiates starch hydrolysis, increases gibberellin concentration and its associated signal transduction, and mobilizes the degradation of storage substances to increase soluble sugar content, thus increasing energy for seed germination and seedling growth (Kaneko et al, 2002;Magneschi and Perata, 2009). We found that α-amylase activity and soluble sugar content gradually increased with germination, which is consistent with results of Nie et al(2022). What is more, α-amylase activity in overexpressing seeds was significantly higher than that in wild-type seeds, but lower in knockout seeds, indicating that OsPIP2-1 positively regulates the activity of α-amylase.…”

Section: Discussionsupporting

confidence: 90%

Nano-silver and forskolin regulate rice seed germination mediated byOsPIP2-1

Chen¹,

Peng

Zhang³

et al. 2023

Preprint

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Herein, we found that nano-silver inhibited rice seed germination while forskolin promoted this process. Germination assay showed that 20 μM AgNPs and 0.5 μM forskolin could be the optimal concentrations regulating rice seed germination. At the molecular level, an aquaporin gene, OsPIP2-1, was most highly expressed among those homologous genes during rice seed germination, and was decreased by AgNPs and increased by forskolin. Meanwhile, the activity of α-amylase and its genes expression were also inhibited by AgNPs and promoted by forskolin. Next, the germination percentage of OsPIP2-1 overexpressing seeds was significantly higher than that of wild-type, and that of OsPIP2-1 knockout seeds was dramatically reduced. Furthermore, compared with the wild type, α-amylase activity and the transcription of OsAmy3C and OsAmy3E were increased in OsPIP2-1 overexpressing seeds and decreased in OsPIP2-1 knockout seeds, indicating that OsPIP2-1 positively regulates α-amylase. Interestingly, seed germination, α-amylase activity and its genes expression in OsPIP2-1 overexpressed and knockdout lines could also be inhibited by nano-silver and promoted by forskolin. In conclusion，we propose that nano-silver and forskolin regulated seed germination by modulating the expression of OsPIP2-1 and thus α-amylase. Our results may bring new application of nano-silver and forskolin in rice cultivation.

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Section: Discussionsupporting

confidence: 90%

Nano-silver and forskolin regulate rice seed germination mediated byOsPIP2-1

Chen¹,

Peng

Zhang³

et al. 2023

Preprint

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“…Recent studies have shown the positive effects of various seed priming methods on rice under chilling stress. For example, Nie et al. (2022) reported that seed priming increases the chilling tolerance of rice seeds during germination and growth by enhancing α-amylase activity and soluble sugar content.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have shown the positive effects of various seed priming methods on rice under chilling stress. For example, Nie et al (2022) reported that seed priming increases the chilling tolerance of rice seeds during germination and growth by enhancing a-amylase activity and soluble sugar content. Furthermore, Zhu et al (2021) showed that priming with salicylic acid (SA), gibberellin (GA 3 ), CaCl 2 , and abscisic acid (ABA) improves the germination potential, germination rate, and seed vigor index of rapeseed under chilling stress, and shortens average germination time.…”

Section: Introductionmentioning

confidence: 99%

Effects of various seed priming on morphological, physiological, and biochemical traits of rice under chilling stress

Zhang

Zhang²,

Gao³

et al. 2023

Front. Plant Sci.

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Introduction/BackgroundDirect-seeded rice is exceptionally vulnerable to chilling stress, especially at the seed germination and seedling growth stages in the early season of the double cropping system.MethodsTherefore, we conducted two experiments to evaluate the role of various seed primings and their different concentrations of plant growth regulators [experiment 1—abscisic acid (ABA), gibberellin (GA3), salicylic acid (SA), brassinolide (BR), paclobutrazol, uniconazole (UN), melatonin (MT), and jasmonic acid (JA)] and osmopriming substances (chitosan, polyethylene glycol 6000 (PEG6000), and CaCl2) and experiment 2—GA, BR (two best), CaCl2 (worst), and control (CK)] on rice seedlings under low temperature condition.ResultsResults showed that the maximum germination rate of 98% was recorded in GA3 (10 mgL−1) and BR (0.3 mgL−1) among treatments. Compared to CK, root and shoot length were improved in ABA (0.5 mgL−1) and GA3 (100 mgL−1) by 64% and 68%, respectively. At the same time, root and shoot weights (fresh and dry) were enhanced in Paclobutrazol (300 mgL−1) and GA3 among treatments. Furthermore, the average root volume, average root diameter, and total root surface area were increased by 27%, 38%, and 33% in Paclobutrazol (300 mgL−1), Paclobutrazol (200 mgL−1) and JA (1 mgL−1) treatments, respectively compared to CK. In the second experiment, a respective increase of 26%, 19%, 38%, and 59% was noted in SOD, POD, CAT, and APX enzyme activities in GA treatment compared to CK. Similarly, proline, soluble sugar, soluble protein, and GA content were also improved by 42%, 25.74%, 27%, and 19%, respectively, in GA treatment compared to CK. However, a respective reduction of 21% and 18% was noted in MDA and ABA content in GA treatment compared to CK. Our finding highlighted that better germination of primed-rice seedlings was associated with fresh and dry weights of the roots and shoots and the average root volume of the seedlings.DiscussionOur results suggested that GA3 (10 mg L−1) and BR (0.3 mg L−1) seed priming prevent rice seedlings from chilling-induced oxidative stress by regulating antioxidant enzyme activities and maintaining ABA, GA, MDA, soluble sugar, and protein content. However, further studies (transcriptome and proteome) are needed to explore the molecular mechanisms involved in seed priming-induced chilling tolerance under field conditions.

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“…29 Under chilling stress, the expression of OsRamy3D in rice seeds was strongly induced by the low level of soluble sugar, which ensured α-amylase synthesis. 30 In this study, GV treatment downregulated the expression level of OsRamy3D under chilling stress, which may be because GV treatment increased the levels of soluble sugar in rice seeds, thereby inhibiting the transcription of OsRamy3D. Previous studies have shown that accelerated starch metabolism in rice by seed priming contributes to an increase in respiration rate, which leads to the generation of more ATP to promote coleoptile and root growth.…”

Section: ■ Discussionmentioning

confidence: 60%

Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling Stress

Liu

Bing

et al. 2023

J. Agric. Food Chem.

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Rice direct seeding technology has been considered as a promising alternative to traditional transplanting because of its advantages in saving labor and water. However, the poor emergence and seedling growth caused by chill stress are the main bottlenecks in wide-scale adoption of direct-seeded rice in Heilongjiang Province, China. Here, we found that natural plant growth regulator guvermectin (GV) effectively improved rice seed germination and seedling growth under chilling stress. Results from 2 year field trials showed that seed-soaking with GV not only enhanced the emergence rate and seedling growth but also increased the panicle number per plant and grain number per panicle, resulting in 9.0 and 6.8% increase in the yield of direct-seeded rice, respectively. Integrative physiological, transcriptomic, and metabolomic assays revealed that GV promoted seed germination under chilling stress mainly by enhancing the activities of α-amylase and antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), increasing the contents of soluble sugar and soluble protein, improving the biosynthesis of glutathione and flavonoids, as well as activating gibberellin-responsive transcription factors and inhibiting the abscisic acid signaling pathway. These findings indicate that seed-soaking with GV has good potential to improve seedling establishment and yield of direct-seeded rice even under chilling stress.

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Enhancement in Seed Priming-Induced Starch Degradation of Rice Seed Under Chilling Stress via GA-Mediated α-Amylase Expression

Cited by 24 publications

References 66 publications

Nano-silver and forskolin regulate rice seed germination mediated byOsPIP2-1

Nano-silver and forskolin regulate rice seed germination mediated byOsPIP2-1

Effects of various seed priming on morphological, physiological, and biochemical traits of rice under chilling stress

Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling Stress

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