Enhancement of Cunninghamella elegans UCP/WFCC 0542 Biomass and Chitosan with Amino Acid Supply

Santos, Ednaldo Ramos dos; Silva, Marta Cristina Freitas da; Souza, Patrícia Mendes de; Silva, Antonio Cardoso da; Paiva, Sérgio Carvalho de; Albuquerque, C. D. C.; Nascimento, Aline Elesbão do; Okada, Kaoru; Campos-Takaki, Galba Maria de

doi:10.3390/molecules180910095

Cited by 7 publications

(15 citation statements)

References 31 publications

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“…Santos et al [10] reported that the increase in biomass production by C. elegans is due to the presence of considerable amounts of amino acids (alanine, arginine, histidine, leucine, lysine, tyrosine, phenylalanine) and vitamins (biotin, choline, inositol, niacin, pyridoxine, thiamine) that are essential for the growth of the microorganism. Edwinoliver et al [29] also suggest that the use of corn steep liquor for lipase production from Aspergillus niger makes the process green, because this substrate is renewable and economically viable on an industrial scale.…”

Section: Resultsmentioning

confidence: 99%

Green Conversion of Agroindustrial Wastes into Chitin and Chitosan by Rhizopus arrhizus and Cunninghamella elegans Strains

Berger

Stamford

Stamford–Arnaud

et al. 2014

IJMS

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This article sets out a method for producing chitin and chitosan by Cunninghamella elegans and Rhizopus arrhizus strains using a green metabolic conversion of agroindustrial wastes (corn steep liquor and molasses). The physicochemical characteristics of the biopolymers and antimicrobial activity are described. Chitin and chitosan were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, viscosity and X-ray diffraction. The effectiveness of chitosan from C. elegans and R. arrhizus in inhibiting the growth of Listeria monocytogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, Escherichia coli and Yersinia enterocolitica were evaluated by determining the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC). The highest production of biomass (24.60 g/L), chitin (83.20 mg/g) and chitosan (49.31 mg/g) was obtained by R. arrhizus. Chitin and chitosan from both fungi showed a similar degree of deacetylation, respectively of 25% and 82%, crystallinity indices of 33.80% and 32.80% for chitin, and 20.30% and 17.80% for chitosan. Both chitin and chitosan presented similar viscosimetry of 3.79–3.40 cP and low molecular weight of 5.08 × 103 and 4.68 × 103 g/mol. They both showed identical MIC and MBC for all bacteria assayed. These results suggest that: agricultural wastes can be produced in an environmentally friendly way; chitin and chitosan can be produced economically; and that chitosan has antimicrobial potential against pathogenic bacteria.

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Section: Resultsmentioning

confidence: 99%

Green Conversion of Agroindustrial Wastes into Chitin and Chitosan by Rhizopus arrhizus and Cunninghamella elegans Strains

Berger

Stamford

Stamford–Arnaud

et al. 2014

IJMS

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“…Recent advances in fermentation technology addressed that many of these problems can be overcome by culturing chitosan-producing fungi, particularly Zygomycetes species, which are known to contain chitosan as natural cell wall component [7]- [10].…”

Section: Introductionmentioning

confidence: 99%

Fermentative Production of Mycelial Chitosan from Zygomycetes: Media Optimization and Physico-Chemical Characterization

Vaingankar¹,

Juvekar²

2014

ABB

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The present study focused on production of mycelial chitosan from fungal mycelium by submerged fermentation with ecologically more balanced process. Different fungal strains were screened and Absidia butleri NCIM 977 was found to produce the highest mycelial chitosan. The one-factor-at-a-time method was adopted to investigate the effect of batch time, environmental factors (i.e. initial pH and temperature) and medium components (i.e. carbon and nitrogen) on the yield of mycelial chitosan. Among these variables, the optimal condition to increase in yield of mycelial chitosan was found to be batch time (72 h), pH (5.5), temperature (30˚C), carbon source (glucose) and nitrogen source (tryptone and yeast extract). Subsequently, a three-level Box-Behnken factorial design was employed combining with response surface methodology (RSM) to maximise yield of mycelial chitosan by determining optimal concentrations and investigating the interactive effects of the most significant media components (i.e. carbon and nitrogen sources). The optimum value of parameters obtained through RSM was glucose (1.58%), tryptone (1.61%) and yeast extract (1.11%). There was an increase in mycelial chitosan yield after media optimization by one-factor-at-a-time and statistical analysis from 683 mg/L to 1 g/L. Mycelial chitosan was characterized for total glucosamine content (80.68%), degree of deacetylation (DD) (79.89%), molecular weight (8.07 × 10 4 Da) and, viscosity (73.22 ml/g). The results of this study demonstrated that fungi are promising alternative sources of chitosan with high DD and high purity.

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“…Valores inferiores de biomassa de C. elegans (IFM 46109) em meio enriquecido com glicose, asparagina e tiamina, também foram relatados por Franco et al (2005), com 11,6 g L -1 . Santos et al (2013) também obtiveram valores menores de biomassa, 16,95 g L -1 , em relação ao presente estudo, ao cultivar C. elegans (UCP/WFCC 0542) em meio composto por milhocina.…”

Section: Produção De Biomassaunclassified

“…Stamford et al (2007), relataram alto rendimento de quitina, 40,09%, contudo, o rendimento de quitosana permaneceu dentro da média, 5,89%. Um rendimento acima da média foi relatado por Santos et al (2013) ao obter 2,14 g L -1 , e aproximadamente 13,8%, de quitosana.…”

Section: Rendimento De Quitina E Quitosanaunclassified

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Biomassa e extração de quitina e quitosana a partir de isolados de Cunninghamella sp.

Lopes¹,

Chagas²,

Scheidt³

et al. 2017

Semin. Cienc. Biol. Saude

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O estudo objetivou avaliar a produção de biomassa de nove isolados de Cunninghamella sp. e da cepa referência de Cunninghamella elegans (CBMAI 0843) e estabelecer a capacidade de produção de quitina e quitosana por estas cepas. Assim como, caracterizar a quitosana fúngica obtida por parâmetros como massa molar, grau de desacetilação e distribuição dos grupos funcionais ao longo da cadeia polimérica. Para a maioria das cepas avaliadas, o período de maior crescimento foi em 48 horas de cultivo, sendo que, neste período, o isolado UFT Ce08 apresentou a maior quantidade de biomassa, 20,17 g L-1. Os rendimentos de quitina ficaram entre 15,64 a 30,33% e os rendimentos de quitosana entre 0,94 a 7,43%. A cepa UFT Ce11 apresentou o melhor quantitativo de quitina e a cepa UFT Ce09, mesmo apresentando o segundo menor quantitativo de biomassa, 9,34 g L-1, teve o melhor rendimento de quitosana. Sete cepas isoladas no presente estudo apresentaram maior rendimento de quitosana comparada à cepa referência. O grau médio de desacetilação foi de 83,7% para quitosana obtida do isolado UFT Ce09 e 80,5% para quitosana obtida da cepa referência. As massas molares para a quitosana do isolado UFT Ce09 e da cepa referência foram de 43,031 e 19,215 g mol-1, respetivamente. A espectrometria de infravermelho apresentou bandas com comprimentos de onda e grupos funcionais coincidentes com a literatura e com a quitosana comercial. A quitosana fúngica deste estudo apresentou propriedades que atestam sua qualidade e características de interesse biotecnológico e comercial.

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Enhancement of Cunninghamella elegans UCP/WFCC 0542 Biomass and Chitosan with Amino Acid Supply

Cited by 7 publications

References 31 publications

Green Conversion of Agroindustrial Wastes into Chitin and Chitosan by Rhizopus arrhizus and Cunninghamella elegans Strains

Green Conversion of Agroindustrial Wastes into Chitin and Chitosan by Rhizopus arrhizus and Cunninghamella elegans Strains

Fermentative Production of Mycelial Chitosan from Zygomycetes: Media Optimization and Physico-Chemical Characterization

Biomassa e extração de quitina e quitosana a partir de isolados de Cunninghamella sp.

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