Enhancement of Cytotoxicity and Apoptosis Induction of Doxorubicin by Brazilein Containing Fraction of Secang (Caesalpinia sappan L.) on T47D Cells

Abstract

Combination chemotherapy (co-chemotherapy) is a recent strategy to reduce the toxicity effect and increase the effectivity of chemotherapeutic agent, such as Doxorubicin (Dox). Caesalpinia sappan L. are potential to be developed as co-chemoterapeutic agents due to its strong cytotoxicity toward several breast cancer cells. The purpose of this research is to observe the cytotoxicity of Brazilein containing fraction (BCF) in single and its combination with doxorubicin on T47D cells. BCF was obtained by fractionation using chloroform:ethyl acetate (40:60 v/v) as mobile phase. Molecular docking results showed that Brazilein and Brazilin interacted with Bcl-2 with different binding properties. Based on MTT assay, Dox and BCF performed potent cytotoxicity with IC50 value of 403 nM and 68 μg/mL, respectively. BCF increased the cytotoxicity of Dox and performed synergism with CI value <1 and decreased possible toxicity with DRI value>1. Under Annexin V PI staining Flowcytometry, BCF in single and its combination with doxorubicin induced apoptosis. In conclusion, single treatment of BCF and its combination with Dox performed cytotoxic effect and induced apoptosis on T47D cell lines.Keywords: Brazilein containing fraction, Doxorubicin, Co-chemoteraphy, Apoptosis, T47D cells

The effect of ethyl acetat fraction of Caesalpinia sappan L. wood on PC3 cancer cell line : cell viability and migration study

“…Fraction-9 also exhibits a higher cytotoxic activity compared to the cytotoxic activity of ethyl acetate fraction reported on HeLa, MCF-7/Moc, MCF-7/Her2, and T47D cancer cells, where the range of IC50 were 40 -65μg/mL. [17][18][19] Investigation on anti-cancer activity of purified brazilin showed a potential anti-cancer activity of brazilin. Kim et al reported high cytotoxic activity of brazilin on MM cells (U266 and MM1s), leukemia cell line (K562) with the range of IC50 10-80μM.…”

Co-treatment of Brazilein Enhances Cytotoxicity of Doxorubicin on WiDr Colorectal Cancer Cells Through Cell Cycle Arrest

“…Dox was expected to have an IC 50 value in less than 1 µM, as reported in its activities against other cancer cell lines. (15,27) Co-treatment with Dox is expected to increase the effectiveness of Dox, especially in dealing with colon cancer, because so far, several colon cancer therapy regimens have not been able to overcome the growing problem of resistance to colon cancer. (18,28) Dox is not currently an option for colon cancer therapy because Dox also has resistance problems and side effects that are difficult to avoid and have stability problems for oral use.…”

“…The inhibitory effect of Be in cell cycle progression seems to be typical for various cancer cells (11,23), which is generally continue to occur apoptosis (15). However, the effect of cell cycle arrest of co-treatment of Be and Dox on WiDr cells does not appear to be related to apoptotic induction.…”

“…Both compounds have vigorous cytotoxic activities in some cancer cells, such as MCF-7 (11), 4T1 (12), MCF-7/HER2 (13), MDA-MB (14), and other types of breast cancer cell line (15). The anticancer mechanism of the two compounds occurred through cell cycle arrest in the G2/M phase and apoptosis tracking (10) and can inhibit cancer cells' migration, which allows both compounds as anti-metastatic agents (14,15). The two compounds also showed synergistic effects with cisplatin on WiDr colon cancer cells.…”

Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang

“…The success story of Paclitaxel from Taxus brevifolia as an anticancer drug on clinical trial induces researchers discovering natural products or its metabolites as novel chemopreventive agents. Several Indonesian natural products such as Caesalpinia sappan, Cinnamomum burmanii, and Nerium indicum have been Submitted: September 13, 2019 Revised: November 11, 2019 Accepted: November 11, 2019 *Corresponding author: desakgedesa@gmail.com explored their distinct chemopreventive mechanism on cancer cell (Larasati, et al, 2014;Lestari, et al, 2017;Utomo, et al, 2018). However, the lack of identification about the secondary metabolite contribute on its chemopreventive activity impacts on Indonesian Journal of Cancer Chemoprevention, October 2019 ISSN: 2088-0197 e-ISSN: 2355-8989 the bias standard for the application of certain natural products.…”