Enhancement of Glucosinolate Production in Watercress (<i>Nasturtium officinale</i>) Hairy Roots by Overexpressing Cabbage Transcription Factors

Cường, Đỗ Mạnh; Park, Chang Ha; Bong, Sun Ju; Kim, Nam Su; Kim, Jae Kwang; Park, Sang Un

doi:10.1021/acs.jafc.9b00440

Cited by 20 publications

(13 citation statements)

References 34 publications

(63 reference statements)

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“…The method and conditions used for real-time PCR have been described in a previous study [26]. For the qRT-PCR assay, sets of gene-specific primers for terpenoid biosynthesis were designed using the Gene Runner program (version 3.05).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in Lavandula pubescens

et al. 2021

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Lavandula pubescens, belonging to the Labiatae family, is a newly discovered strongly aromatic species of lavender that is potentially beneficial for human health. Given the economic importance of lavender species, we sought in this study to characterize the terpenoid biosynthesis of L. pubescens by obtaining transcriptomic and metabolic datasets. Transcriptome analysis of L. pubescens grown aseptically in tissue culture medium yielded 124,233 unigenes with an average length of 470 bp and N50 value of 522 bp from 9,476,122,928 raw reads. In order to provide relevant biological information, the unigenes were annotated using the following public databases: National Center for Biotechnology Information (NCBI) nucleotide (NT) and non-redundant protein (NR), Brassica (BRAD), Arabidopsis Information Resource (TAIR), Clusters of Orthologous Groups (COG), and Gene Ontology (GO). NR annotation results revealed that L. pubescens is genetically closely related to Sesamum indicum. On the basis of the transcriptome data, a total of 14 cDNA clones encoding the terpene biosynthetic genes LpDXS, LpMCT, LpMCS, LpHDR, LpIDI, LpAACT, LpHMGS, LpHMGR, LpMVK, LpPMK, LpMVD, LpGPPS, LpSQS, and LpGGPPS were identified in L. pubescens. These were quantified in the roots, stems, and leaves of L. pubescens using quantitative real-time polymerase chain reaction (qRT-PCR), which revealed that the gene expression levels were higher in the leaves and stems than in the roots, which was found to be consistent with the levels of ursolic and oleanolic acids in the different organs using high-performance liquid chromatography (HPLC). A total of 48 hydrophilic metabolites were identified and quantified in the organs using gas chromatography time-of-flight mass spectrometry (GC-TOFMS). Furthermore, the antioxidant activity of an ethyl acetate extract of L. pubescens leaves was examined using different methods to determine the potential therapeutic properties. A reducing power assay revealed that the absorbance values increased in a concentration-dependent manner, whereas a 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay indicated the strong activity (60.4 ± 0.9%) of the ethyl acetate extract at a concentration of 100 µg/mL, which also showed strong hydrogen peroxide (57.4 ± 2.7%), superoxide radical (62.1 ± 0.7%), and hydroxyl radical (58.6 ± 0.4%) scavenging activities.

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Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in Lavandula pubescens

et al. 2021

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“…Genomic DNA of three ZmLC -transgenic lines and one GUS -transgenic hairy root line of Tartary buckwheat were isolated using a plant DNA extraction kit (Geneaid Biotech Ltd., Taipei, Taiwan). The primers for rol A–D and bar genes and conditions for the PCR reaction were based on a previous study [ 35 ]. PCR products of the expected lengths (500, 360, 900, 514, and 1035 bp) of bar and rol A, B, C, and D, respectively, were separated on a 1% agarose gel (data not shown).…”

Section: Methodsmentioning

confidence: 99%

“…HPLC analysis of phenolic compounds in ZmLC - and GUS -transgenic hairy root lines of Tartary buckwheat was performed according to the method reported by Park et al [ 35 ]. Briefly, 1.5 mL of 80% ( v/v ) aqueous methanol was added to a tube containing 100 mg of three ZmLC -transgenic and one GUS -transgenic hairy root line and vortexed for 1 min.…”

Section: Methodsmentioning

confidence: 99%

“…Phenolics were separated using methanol (A) and 0.2% acetic acid-water (B). The HPLC system (NS-4000, Futecs Co., Daejeon, Korea), column (OptimaPak, 250 × 4.6 mm, 5 µm, RStech Co., Daejeon, Korea), and analysis conditions were obtained from a previous study [ 35 ]. Comparison of retention time and spiking tests were performed to identify the phenolics, and the calibration curves for each phenolic compound were used to determine the concentration in the samples.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Light and Dark on the Phenolic Compound Accumulation in Tartary Buckwheat Hairy Roots Overexpressing ZmLC

Park

Yeo

et al. 2021

IJMS

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Fagopyrum tataricum ‘Hokkai T10′ is a buckwheat cultivar capable of producing large amounts of phenolic compounds, including flavonoids (anthocyanins), phenolic acids, and catechin, which have antioxidant, anticancer, and anti-inflammatory properties. In the present study, we revealed that the maize transcription factor Lc increased the accumulation of phenolic compounds, including sinapic acid, 4-hydroxybenzonate, t-cinnamic acid, and rutin, in Hokkai T10 hairy roots cultured under long-photoperiod (16 h light and 8 h dark) conditions. The transcription factor upregulated phenylpropanoid and flavonoid biosynthesis pathway genes, yielding total phenolic contents reaching 27.0 ± 3.30 mg g−1 dry weight, 163% greater than the total flavonoid content produced by a GUS-overexpressing line (control). In contrast, when cultured under continuous darkness, the phenolic accumulation was not significantly different between the ZmLC-overexpressing hairy roots and the control. These findings suggest that the transcription factor (ZmLC) activity may be light-responsive in the ZmLC-overexpressing hairy roots of F. tataricum, triggering activation of the phenylpropanoid and flavonoid biosynthesis pathways. Further studies are required on the optimization of light intensity in ZmLC-overexpressing hairy roots of F. tataricum to enhance the production of phenolic compounds.

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“…Genomic DNA of seedling roots and hairy roots of tan and purple seed coat peanuts was extracted using the Plant Genomic DNA Mini Kit (Geneaid Biotech Ltd., Taipei, Taiwan). The primers for identifying fragments of rolA (360 bp), rolB (900 bp), rolC (514 bp), and rolD (1035 bp) were designed in reference to a previous study [37]. The settings for thermal cycling conditions were as follows: initial denaturation at 95 • C for 10 min, 30 cycles of amplification at 95 • C for 10 s, followed by primer annealing at 55 • C for 30 s, primer extension at 72 • C for 1 min, and final extension at 72 • C for 10 min and cooling at 4 • C. Gel electrophoresis was used to verify the expected lengths (360, 900, 514, and 1035 bp) of the targeted gene sequences (rol A, B, C, and D, respectively).…”

Section: Extraction Of Genomic Dna and Polymerase Chain Reaction (Pcr) Analysismentioning

confidence: 99%

Resveratrol Biosynthesis in Hairy Root Cultures of Tan and Purple Seed Coat Peanuts

Park

Yeo

et al. 2021

Agronomy

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Peanut (Arachis hypogaea) is a crop that can produce resveratrol, a compound with various biological properties, such as those that exert antioxidant, anticancer, and anti-inflammatory effects. In this study, trans-resveratrol was detected in the roots, leaves, and stems of tan and purple seed coat peanuts (Arachis hypogaea) cultivated in a growth chamber. Both cultivars showed higher levels of resveratrol in the roots than the other plant parts. Thus, both cultivars were inoculated with Agrobacterium rhizogenes, in vitro, to promote hairy root development, thereby producing enhanced levels of t-resveratrol. After 1 month of culture, hairy roots from the two cultivars showed higher levels of fresh weight than those of seedling roots. Furthermore, both cultivars contained higher t-resveratrol levels than those of their seedling roots (6.88 ± 0.21 mg/g and 28.07 ± 0.46 mg/g, respectively); however, purple seed coat peanut hairy roots contained higher t-resveratrol levels than those of tan seed coat peanut hairy roots, ranging from 70.16 to 166.76 mg/g and from 46.61 to 54.31 mg/g, respectively. The findings of this study indicate that peanut hairy roots could be a good source for t-resveratrol production due to their rapid growth, high biomass, and substantial amount of resveratrol.

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Enhancement of Glucosinolate Production in Watercress (Nasturtium officinale) Hairy Roots by Overexpressing Cabbage Transcription Factors

Cited by 20 publications

References 34 publications

Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in Lavandula pubescens

Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in Lavandula pubescens

Effect of Light and Dark on the Phenolic Compound Accumulation in Tartary Buckwheat Hairy Roots Overexpressing ZmLC

Resveratrol Biosynthesis in Hairy Root Cultures of Tan and Purple Seed Coat Peanuts

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