Enhancement of liver microsomal acyl CoA‐lysophospholipid acyltransferase activity in pyridoxine‐deficient rats

Abstract

In rats deficient in pyridoxine and essential fatty acids, liver phospholipids contained less arachidonic acid and more oleic and eicosatrienoic acids than those from animals only deficient in essential fatty acids. This pattern persisted after the animals were supplemented with linoleate for 6 days. Liver oleyl and arachidonyl CoA‐lysophospholipid acyltransferase activities were significantly higher in pyridoxine‐deficient animals. Supplementation with linoleate for 6 days resulted in a marked increase in arachidonyl CoA‐acyltransferase activity in pyridoxine‐deficient rats but a relatively small increase in the supplemented animals. Differences in fatty acid composition between pyridoxine‐deficient and supplemented rats can not be ascribed to lower liver transacylase activity in the deficient animals.

Marginal Vitamin B-6 Deficiency Decreases Plasma (n-3) and (n-6) PUFA Concentrations in Healthy Men and Women3

“…The changes of plasma (n-6) and (n-3) PUFA observed in the present study apparently were not of sufficient magnitude or duration to alter the cellular fatty acid profiles in this 28-d vitamin B-6 restriction in which steady state likely would not have been attained. The small and nonsignificant change in PBMC oleic acid reported here is similar to the response of erythrocyte membrane lipids reported in rats fed a vitamin B-6-free diet for 20 wk, which is a much more severe deficiency (41). In that study, liver oleyl and arachidonyl CoAlysophospholipid acyltransferase activities were significantly higher in vitamin B-6-deficient rats than in pair-fed controls, suggesting that more arachidonate and oleate were incorporated into membrane phospholipids.…”