Enhancement Of Pneumo-4 Vaccine Efficacy By Using Of Carbomere As An Adjuvant

Abstract

The present study aimed to prepare a combined inactivated pneumo-4 vaccine containing bovine viral diarrhoea, infectious bovine rhinotracheitis (IBR), parainfluenza-3(PI-3) and bovine respiratory syncytial virus (BRSV) adjuvanted with carbomer and compare the animal protection scope with its product mate Pneumo-4 adjuvanted alhydragel . Carbomer adjuvant stimulated higher levels and longer lasting antibody to pneumo-4 vaccine than vaccines of equivalent antigenic content containing aluminium hydroxide gel adjuvant. Quality control results proved that the pneumo-4 vaccine adjuvanted either by aluminium hydroxide gel adjuvant or carbomer (different concentration 0.2,0.5 and0.8 %) was pure and completely safe to be used in calves without any abnomalities.Potency test was performed on five groups of five calves for each group,Where the first group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.2(formula 2), second group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.5 (formula 3), third group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.8(formula 4),,The fourth group vaccinated with pneumo-4 vaccine with aluminum hydroxide gel(formula 1), and the fifth last group was left as non vaccinated control group.compared humoral immune response using serum neutralization and ELISA expressed by neutralization index of different vaccine formulation was revealed that The prepared pneumo-4 vaccine adjuvant with carbomer 0.5 proved to be highly potent formmula along 6 month after second booster dose applying. Also pneumo-4 vaccine adjuvant with carbomer was showed cellular immunity reaction higer than pneumo-4 vaccine with aluminum hydroxide gel using Estimation of nitric oxide activity test.‫ـــــــــــــــــــــــــــــــــــــــــ‬

Efficacy and durability of bovine virus diarrhea (BVD) virus killed vaccine adjuvanted with monolaurin

“…When montanide oil (ISA 206) was used [ 16 , 34 ] as an adjuvant, they found that it had a large safety margin with higher neutralizing antibody titers for longer durations compared to the commercial vaccine since it can trap the antigen and release it over long periods and increase the physical presentation of the antigen. The Pneumo-4 vaccine adjuvanted with carbomer 0.5% [ 17 ] showed high potency with the stimulation of cellular immunity compared to the vaccine adjuvanted with aluminum hydroxide gel.…”

“…Ten Albino Swiss weaned mice weighing 10–15 g were used in running safety tests of the prepared vaccine formulae [ 17 ]. They were obtained, reared, and observed in the Laboratory Animals Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.…”

“…Susceptible healthy native breed male calves aged 6 months were used to evaluate the potency and the safety of different vaccine formulas [ 17 ]. Animals were tested using the neutralization test for both BVDV antigens and antibodies to be proof its freedom from both.…”