Enhancement of serum fibronectin adsorption and the clonal plating efficiencies of Swiss mouse 3T3 fibroblast and MM14 mouse myoblast cells on polymer substrates modified by radiofrequency plasma deposition

Chinn, Joseph A.; Horbett, Thomas A.; Ratner, Buddy D.; Schway, Michael B.; Haque, Yasmeen; Hauschka, Stephen D.

doi:10.1016/0021-9797(89)90008-8

Cited by 94 publications

(30 citation statements)

References 37 publications

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“…However, Fn has been shown to preferentially absorb from diluted serum onto less hydrophobic surfaces. The maximum in adsorption correlates with the maximum in cell attachment and spreading (11,18). However, there are inconsistencies in explaining the exact role of Fn in cell attachment and growth.…”

Section: Discussionmentioning

confidence: 96%

“…Since protein adsorption from serum containing media occurs rapidly (12), direct contact of cells onto the polymer surface is unlikely to occur unless serum is absent. Functional groups present on the polymer surface are believed to affect cell behavior due to differences in the adsorption and conformation of proteins (11,(18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

“…However, determination of the specific functionalities involved in cell growth is difficult due to the number of different groups introduced onto the surface. The result is that various chemical groups have been proposed to promote cell attachment (9)(10)(11). There are few studies in the literature which specifically look at the cellular attachment process to these types of surface.…”

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Effects of Serum on the Kinetics of CHO Attachment to Ultraviolet–Ozone Modified Polystyrene Surfaces

Teare

Emmison

Ton-That

et al. 2001

Journal of Colloid and Interface Science

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Effects of Serum on the Kinetics of CHO Attachment to Ultraviolet–Ozone Modified Polystyrene Surfaces

Teare

Emmison

Ton-That

et al. 2001

Journal of Colloid and Interface Science

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“…In addition, the initial spreading of 3T3 cells on HEMA-EMA copolymers is linearly correlated with the amount of fibronectin adsorbed to the surfaces from serum (61). Similarly, plasma deposited polymers which enhanced 3T3 and muscle myoblast cell growth also enhanced fibronectin adsorption from serum in comparison to untreated surfaces (63). Depletion studies, in which the fibronectin or vitronectin are selectively removed from the serum, have shown that vitronectin, rather than fibronectin, appears to be the primary adhesion factor for certain cells and surfaces (62,(64)(65)(66).…”

Section: The Role Of Adsorbed Proteins In Cell Interactions With Solimentioning

confidence: 90%

Proteins at Interfaces

Brash

Horbett

1995

ACS Symposium Series

204

119

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Proteins at interfaces are involved in a wide variety of phenomena, including mammalian cell growth in culture, reactions to implanted biomaterials, growth of soil bacteria, and formation of organized layers of proteins at the oil/water and air/water interfaces.This broad range of phenomena has attracted the interest of a correspondingly broad range of scientists and engineers, many with different backgrounds and different perspectives. As a result, the field of proteins at interfaces displays as much breadth as depth, and many of the investigators in this field of research conduct investigations that are unique with respect both to the particular protein/surface system studied and to the methods used. The many different approaches and systems under investigation, and in particular the wide variety of applications towards which the research is directed, means that an overview such as this one which is limited in length, must be selective. In selecting subtopics for discussion we had to be somewhat arbitrary, and therefore tried to focus on broad aspects which are common to most application areas.The contributions to the San Diego Symposium and to this volume represent a significant fraction of the workers who are actively involved in studying the behavior of proteins at interfaces. We have therefore chosen to organize our overview around six major aspects of protein behavior at interfaces which emerged from the Symposium. These aspects are: (1) theory, including molecular mechanisms; (2) competitive adsorption; (3) conformation and orientation of proteins at interfaces; (4) surface chemistry effects on adsorption; (5) the behavior of proteins at fluid interfaces; and (6) effects of proteins on cell interactions with surfaces.For each topic, current understanding is briefly summarized, and the contributions of the articles in this volume are then discussed. Inevitably this type of subdivision is to some extent arbitrary and artificial, so there is some overlap of material from one section to another. We trust, however, that on balance our approach will facilitate the readers' (as well as the authors') task of understanding the state of the art in this field.0097-6156/95/0602-0001$12.75/0

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“…25 Perfluoropropylene ͑C 3 F 6 ͒ ͑Matheson Gas Products, Newark, CA͒ was used as the precursor. During deposition, a pressure of 150 mTorr and a flow rate of 4 sccm were maintained under 20 W power for 3 min to obtain the fluoropolymer coating.…”

Section: B Plasma Depositionmentioning

confidence: 99%

Temperature dependent activity and structure of adsorbed proteins on plasma polymerized N-isopropyl acrylamide

et al. 2006

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Thorough studies of protein interactions with stimulus responsive polymers are necessary to provide a better understanding of their applications in biosensors and biomaterials. In this study, protein behavior on a thermoresponsive polymer surface, plasma polymerized N-isopropyl acrylamide (ppNIPAM), is investigated using multiple characterization techniques above and below its lower critical solution temperature (LCST). Protein adsorption and binding affinity are probed using radiolabeled proteins. Protein activity is estimated by measuring the immunological activity of an antibody adsorbed onto ppNIPAM using surface plasmon resonance. Conformation/orientation of the proteins is probed by time-of-flight secondary ion mass spectrometry (TOF-SIMS) and principal component analysis (PCA) of the TOF-SIMS data. In this work, we find that at low protein solution concentrations, ppNIPAM-treated surfaces are low fouling below the LCST, but protein retentive above it. The protein adsorption isotherms demonstrate that apparent affinity between soluble protein molecules and the ppNIPAM surface are an order of magnitude lower at room temperature than at 37 °C. Although direct protein desorption is not observed in our study when the surface temperature drops below the LCST, the binding affinity of surface adsorbed protein with ppNIPAM is reduced, as judged by a detergent elution test. Furthermore, we demonstrated that proteins adsorbed onto ppNIPAM are functionally active, but the activity is better preserved at room temperature than 37 °C. The temperature dependent difference in protein activity as well as TOF-SIMS and PCA study suggest that proteins take different conformations/orientations after adsorption on ppNIPAM above and below the LCST.

show abstract

Enhancement of serum fibronectin adsorption and the clonal plating efficiencies of Swiss mouse 3T3 fibroblast and MM14 mouse myoblast cells on polymer substrates modified by radiofrequency plasma deposition

Cited by 94 publications

References 37 publications

Effects of Serum on the Kinetics of CHO Attachment to Ultraviolet–Ozone Modified Polystyrene Surfaces

Effects of Serum on the Kinetics of CHO Attachment to Ultraviolet–Ozone Modified Polystyrene Surfaces

Proteins at Interfaces

Temperature dependent activity and structure of adsorbed proteins on plasma polymerized N-isopropyl acrylamide

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