“…Due to the target property to specific tissue and drug controllable release [20], also the general properties of nano materials, such as surface effect [21], small size effect [22], quantum size effect [23], CS nanoparticles have been widely studied for the drug delivery [24] and gene carrier [25]. Up to now, CS nanoparticles were generally prepared by ionic crosslinking method, self-assembled method and emulsification-crosslinking method.…”