Enhancing cell membrane phase separation for inhibiting cancer metastasis with a stimuli-responsive DNA nanodevice

Abstract

Phase separation at cell membranes promotes the assembly of transmembrane receptors to initiate signal transduction in response to environmental cues. Many cellular behaviors are manipulated by promoting membrane phase separation...

Oncolytic Hairpin DNA Pair: Selective Cytotoxic Inducer through MicroRNA-Triggered DNA Self-Assembly

“…Another possible reason could be that miR-21 expression levels are too high to produce enough length of DNA duplex mimic for immune activation. The cytotoxicity of oHP (10), which has a longer toehold and larger background signal, was tested to investigate the mechanism of action for selective cytotoxicity (Figure S6). The pair with a longer toehold showed high cytotoxicity for miR-21-negative HEK-293T but mild cytotoxicity for miR-21positive HeLa cells, suggesting that oHP(10) was consumed for both the background reaction and miR-21-triggered HCR in HeLa cells and the products were not long enough for exerting high cytotoxicity.…”

“…9 Recently, Li and co-workers applied ATP-triggered HCR to inhibit cancer metastasis. 10 In that case, a long nicked DNA duplex was formed, not inside the cell but on the cell membrane, and four different DNA probes were required for metastasis inhibition.…”

Advances and prospects of dynamic DNA nanostructures in biomedical applications

“…This device is initiated by ATP and enhances membrane phase separation through clustering of dynamic lipid ras to obtain the function of inhibiting cancer cell migration in vitro and in vivo. 94 5 Challenges and prospect Stimulus-responsive DDNs not only possess the excellent biological properties of DNA nanostructures, but also enable the intelligent release of targeted biomedical molecules in response to exogenous and endogenous stimuli. Thus, they have a wide range of applications in biosensing, bioimaging, drug delivery, tissue regeneration, and cellular behavior regulation, etc.…”

“…This device is initiated by ATP and enhances membrane phase separation through clustering of dynamic lipid rafts to obtain the function of inhibiting cancer cell migration in vitro and in vivo . 94 …”

Near-Infrared-Light-Mediated DNA-Logic Nanomachine for Bioorthogonal Cascade Imaging of Endogenous Interconnected MicroRNAs and Metal Ions

“…On the other hand, DNAzymes, a class of functional DNA that possess metal-ion-dependent catalytic activity, have been extensively investigated as building units of DNA nanoprobes for metal ion imaging. − In addition, numerous signal amplification technologies, , including rolling circle amplification, , catalytic DNA hairpin assembly, − and hybridization chain reaction (HCR), − have been introduced for designing DNA nanoprobes for the amplified imaging of low abundance of endogenous miRNAs or metal ions in vitro and in vivo . Also, several approaches for controlling the intracellular activation of DNA nanoprobes by various internal or external stimuli have been reported for spatiotemporally controlled purposes. − More recently, multiplexed molecular imaging in vivo has been realized by integration with DNA logic gate systems. − Despite these successes, to our best knowledge, a multiplexed DNA nanoprobe for bioorthogonal controlled visualization of miRNAs and metal ions expressions within the context of in situ tumors is currently lacking.…”