Enhancing methyl parathion degradation by the immobilization of Burkholderia sp. isolated from agricultural soils

Abstract

Organophosphate pesticides are of great interest for research because they are currently the most commonly used pesticides. In this study, a bacterial strain capable of completely degrading methyl parathion (MP) was isolated from agricultural soils in central Mexico. This strain was designated strain S5‐2 and was identified as Burkholderia cenocepacia. To increase degradation yields, cells were immobilized on three different supports: powdered zeolite and Opuntia sp. and Agave sp. fibers. The results indicated a significant increase in MP hydrolysis and p‐nitrophenol (PNP) degradation with immobilized cells compared to free cell cultures. Furthermore, immobilized cells were capable of withstanding and degrading higher concentrations of PNP compared to cell suspension cultures. The cell viability in the free cell cultures, as well as PNP degradation, was affected at concentrations greater than 25 mg/L. In contrast, cells immobilized on Opuntia sp. and Agave sp. fibers completely degraded PNP at concentrations of 100 mg/L. To verify that MP solution toxicity was decreased by B. cenocepacia strain S5‐2 via pesticide degradation, we measured the acetylcholinesterase activity, both before and after treatment with bacteria. The results demonstrate that the activity of acetylcholinesterase was unaffected after MP degradation by bacteria.

Glyphosate induced toxicity to chickpea plants and stress alleviation by herbicide tolerant phosphate solubilizing Burkholderia cepacia PSBB1 carrying multifarious plant growth promoting activities

“…Apart from these, B. cepacia strain CH9 is also known to reduce the toxicity of pesticides by degrading them. For example, the degradation of imidacloprid and metribuzin (Gopal et al 2011) and parathion (Fernández-López et al 2017) following B. cepacia application is reported. Considering these, the glyphosate tolerant B. cepacia PSBB1 was included in this study as inoculant for augmenting chickpea production in glyphosate treated soils.…”

Current status of pesticide effects on environment, human health and it’s eco-friendly management as bioremediation: A comprehensive review

“…Now, research has shifted to the use of microbial cells as CI, which protects and allows them to be reused. Such a strategy enhances the possibilities of techniques lasting and succeeding in a pesticide-contaminated environment for a long period and has been found suitable for pesticide biodegradation ( Colla et al, 2014 ; Pradeep and Subbaiah, 2016 ; Fernández-López et al, 2017 ; Conde-Avila et al, 2021 ).…”

Soil Microorganisms and Their Potential in Pesticide Biodegradation; A Review Article

“…Mangrove peat (Natália Alvarenga et al, 2018) Burkholderia sp. (Fernández-López et al, 2017;Popoca-Ursino, Martinez-Ocampo, Dantan-Gonzalez, Sanchez-Salinas and Ortiz-Hernandez, 2017) Klebsiella sp. (Pattanayak, Chakraborty, Biswas, Chattopadhyay and Chakraborty, 2018) Fischerella sp.…”