Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations

Abstract

Combination chemotherapy is an important protocol in glioma therapy and honokiol shows synergistic anticancer effects with doxorubicin. In this paper, honokiol (HK) and doxorubicin (Dox) co-loaded Methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) nanoparticles were prepared with a assembly method. The particle size (about 34 nm), morphology, X-ray Powder Diffraction (XRD), in vitro release profile, cytotoxicity and cell proliferation effects were studied in detail. The results indicated that honokiol and doxorubicin could be efficiently loaded into MPEG-PCL nanoparticles simultaneously, and could be released from the micelles in an extended period in vitro. In addition, honokiol and doxorubicin loaded in MPEG-PCL nanoparticles could efficiently suppress glioma cell proliferation and induce cell apoptosis in vitro. Furthermore, Dox-HK-MPEG-PCL micelles inhibited glioma growth more significantly than Dox-MPEG-PCL and HK-MPEG-PCL in both nude mice and zebrafish tumor models. Immunohistochemical analysis indicated that DOX-HK-MPEG-PCL micelles improved Dox’s anti-tumor effect by enhancing tumor cell apoptosis, suppressing tumor cell proliferation, and inhibiting angiogenesis. Our data suggest that Dox-HK-MPEG-PCL micelles have the potential to be applied clinically in glioma therapy.

Mixed Lanthanide Oxide Nanoparticles Coated with Alginate-Polydopamine as Multifunctional Nanovehicles for Dual Modality: Targeted Imaging and Chemotherapy

“…Similarly, a concentration-dependent fluorescence intensity was also observed with zebrafish larvae (Figure C). These results indicate that Tb/Eu@AlgPDA NPs were effectively introduced into embryos and larvae through swallowing and skin absorption and that the embryos remained alive …”

Utilizing Edible Agar as a Carrier for Dual Functional Doxorubicin-Fe3O4 Nanotherapy Drugs

“…Therefore, biocompatible agar, as a drug carrier, has been confirmed to have the ability to encapsulate Fe 3 O 4 magnetic particles. Simultaneously, suggesting that DOX exists in the amorphous form in DOX-agar and DOX-Fe 3 O 4 @agar [ 25 , 26 , 27 , 28 , 29 ]. The Fe 3 O 4 particle size calculated by Scherrer’s formula was 9.2 nm, which was consistent with the particle size observed in TEM image.…”

Radiotherapy for glioblastoma: clinical issues and nanotechnology strategies

“…71 In addition to platinum drugs, other chemotherapeutic drugs, such as doxorubicin, paclitaxel and TMZ, have also been loaded into drug delivery carriers to enhance radiosensitivity. [72][73][74] Targeting GSCs…”