Enhancing the fermentation of acidified food waste using a self-formulated thermophilic and acid-resistant bacterial agent

Liu, Dandan; Ma, Xinxin; Huang, Juntao; Shu, Zhifei; Chu, Xu; Li, Yangyang; Jin, Yiying

doi:10.1016/j.jece.2022.107350

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…to make complex microbial agents can significantly shorten the composting cycle and improve the degradation rate of sludge, garden waste, food waste, etc. ( Fang et al, 2019 ; Liu et al, 2022a , b ; Wang M. et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation of starch and protein degrading strain Bacillus subtilis FYZ1-3 from tobacco waste and genomic analysis of its tolerance to nicotine and inhibition of fungal growth

Ye,

Liu,

Huang

et al. 2023

Front. Microbiol.

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Aerobic fermentation is an effective technique for the large-scale processing of tobacco waste. However, the specificity of the structure and composition of tobacco-derived organic matter and the toxic alkaloids in the material make it currently difficult to directly use microbial agents. In this study, a functional strain FYZ1-3 was isolated and screened from thermophilic phase samples of tobacco waste composting. This strain could withstand temperatures as high as 80°C and grow normally at 0.6% nicotine content. Furthermore, it had a strong decomposition capacity of tobacco-derived starch and protein, with amylase activity of 122.3 U/mL and protease activity and 52.3 U/mL, respectively. To further understand the mechanism of the metabolic transformation of the target, whole genome sequencing was used and the secondary metabolite gene cluster was predicted. The inhibitory effect of the strain on common tobacco fungi was verified using the plate confrontation and agar column methods. The results showed that the strain FYZ1-3 was Bacillus subtilis, with a genome size of 4.17 Mb and GC content of 43.68%; 4,338 coding genes were predicted. The genome was annotated and analyzed using multiple databases to determine its ability to efficiently degrade starch proteins at the molecular level. Moreover, 14 functional genes related to nicotine metabolism were identified, primarily located on the distinct genomic island of FYZ1-3, giving a speculation for its nicotine tolerance capability on the molecular mechanism. By mining the secondary metabolite gene cluster prediction, we found potential synthetic bacteriocin, antimicrobial peptide, and other gene clusters on its chromosome, which may have certain antibacterial properties. Further experiments confirmed that the FYZ1-3 strain was a potent growth inhibitor of Penicillium chrysogenum, Aspergillus sydowii, A. fumigatus, and Talaromyces funiculosus. The creation and industrial use of the functional strains obtained in this study provide a theoretical basis for its industrial use, where it would be of great significance to improve the utilization rate of tobacco waste.

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Section: Introductionmentioning

confidence: 99%

Isolation of starch and protein degrading strain Bacillus subtilis FYZ1-3 from tobacco waste and genomic analysis of its tolerance to nicotine and inhibition of fungal growth

Ye,

Liu,

Huang

et al. 2023

Front. Microbiol.

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Solid-state fermentation of cassava (Manihot esculenta Crantz): a review

Egbune,

Ezedom,

Orororo

et al. 2023

World J Microbiol Biotechnol

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Successful Formulation and Application of Low-Temperature Bacterial Agents for Corn Stover Degradation

Zhang

Han

et al. 2023

Agronomy

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Solid bacterial agents are required to accelerate stover degradation in low-temperature areas. However, the laboratory-to-practice translation of bioprocessing techniques is hindered by high cost, poor practicality, and short shelf life. Using corn stover powder, starch, and bran as additives, we screened Pseudomonas putida and Acinetobacter lwoffii, which effectively degrades corn stover at low temperatures, to develop a sustainable and low-cost bacterial agent formula that ensures bacterial viability in low-temperature soil and storage. The optimal formulation included precipitates and additives at a 1:4 ratio, including corn stover powder, starch, and bran at a 4:3:9 ratio. The viable bacterial count with this formulation reached 7.5 × 1010 colony-forming units/g, with high lignocellulase activities. The degradation effect of the optimal formulation on stover and its components, in both lab soil culture simulation and the field environment, was significantly higher than that without bacterial agent application. This formulation had an outstanding effect on lignin. The optimal storage conditions included vacuum packing under 10% water content at 4 °C; the survival rate of viable bacteria reached 85.33% after 180 d. Given the global value of stover-return agriculture, our results offer a valuable strategy for application in low-temperature soils where stover degradation rates are otherwise low.

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Enhancing the fermentation of acidified food waste using a self-formulated thermophilic and acid-resistant bacterial agent

Cited by 4 publications

References 30 publications

Isolation of starch and protein degrading strain Bacillus subtilis FYZ1-3 from tobacco waste and genomic analysis of its tolerance to nicotine and inhibition of fungal growth

Isolation of starch and protein degrading strain Bacillus subtilis FYZ1-3 from tobacco waste and genomic analysis of its tolerance to nicotine and inhibition of fungal growth

Solid-state fermentation of cassava (Manihot esculenta Crantz): a review

Successful Formulation and Application of Low-Temperature Bacterial Agents for Corn Stover Degradation

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