Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich<i>Ecklonia cava</i>extract Seanol<sup>®</sup>to endow antibacterial properties and promote mineralization

Douglas, Timothy; Dokupil, Agnieszka; Reczyńska, Katarzyna; Brackman, Gilles; Krok-Borkowicz, Małgorzata; Keppler, Julia K.; Božič, Mojca; Voort, Pascal Van Der; Pietryga, Krzysztof; Samal, Sangram Keshari; Balcaen, Lieve; Bulcke, Jan Van den; Acker, Joris Van; Vanhaecke, Frank; Schwarz, Karin; Coenye, Tom; Pamuła, Elżbieta

doi:10.1088/1748-6041/11/4/045015

Cited by 24 publications

(30 citation statements)

References 38 publications

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“…Our results appear to also support a previously reported interaction between ALP and polyphenols from the seaweed-derived nutritional supplement, Seanol ® [Douglas et al, 2016]. Here, a similar concentration of polyphenols was used in supplements, which were shown to have reduced release rates during ALP-mediated mineralization.…”

Section: Discussionsupporting

confidence: 91%

Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities

Carson

Keppler

Brackman

et al. 2018

Cells Tissues Organs

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Polyphenols are known for their antimicrobial activity, whilst both polyphenols and the globular protein β-lactoglobulin (bLG) are suggested to have antioxidant properties and promote cell proliferation. These are potentially useful properties for a tissue-engineered construct, though it is unknown if they are retained when both compounds are used in combination. In this study, a range of different microbes and an osteoblast-like cell line (human fetal osteoblast, hFOB) were used to assess the combined effect of: (1) green tea extract (GTE), rich in the polyphenol epigallocatechin gallate (EGCG), and (2) whey protein isolate (WPI), rich in bLG. It was shown that approximately 20–48% of the EGCG in GTE reacted with WPI. GTE inhibited the growth of Gram-positive bacteria, an effect which was potentiated by the addition of WPI. GTE alone also significantly inhibited the growth of hFOB cells after 1, 4, and 7 days of culture. Alternatively, WPI significantly promoted hFOB cell growth in the absence of GTE and attenuated the effect of GTE at low concentrations (64 µg/mL) after 4 and 7 days. Low concentrations of WPI (50 µg/mL) also promoted the expression of the early osteogenic marker alkaline phosphatase (ALP) by hFOB cells, whereas GTE inhibited ALP activity. Therefore, the antioxidant effects of GTE can be boosted by WPI, but GTE is not suitable to be used as part of a tissue-engineered construct due to its cytotoxic effects which negate any positive effect WPI has on cell proliferation.

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Section: Discussionsupporting

confidence: 91%

Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities

Carson

Keppler

Brackman

et al. 2018

Cells Tissues Organs

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“…Generally speaking, values decreased in the order A ≈ B > C > D > E > > GG. It is known from previous work that the values for unmineralized GG samples range between approximately 0% and 2% (Douglas, Dokupil, et al, ). As expected, these values are considerably lower than those for mineralized samples.…”

Section: Resultsmentioning

confidence: 97%

Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions

Lopez‐Heredia

Łapa

Reczyńska

et al. 2018

J Tissue Eng Regen Med

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Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration. In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution. This alternate soaking cycle was repeated five times. Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared. Carbonate mineral formed in all sample groups subjected to the alternate soaking cycle. Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution. Mineral formed in the absence of Mg was predominantly CaCO in the form of a mixture of calcite and vaterite. Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity. Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.

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“…When included in gellan gum hydrogels in the presence of alkaline phosphatase, seanol, a seaweed extract rich in phlorotannins, induced mineralization with calcium phosphate uptake and produced an antibacterial activity [178]. Alginate-lignin aerogels that proved non-cytotoxic and with good cell adhesion properties are also attractive candidates for applications in this field [179].…”

Section: Main Applications Of Phenol-based Polymers In Food and Hementioning

confidence: 99%

Natural Phenol Polymers: Recent Advances in Food and Health Applications

Panzella

Napolitano

2017

Antioxidants

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Natural phenol polymers are widely represented in nature and include a variety of classes including tannins and lignins as the most prominent. Largely consumed foods are rich sources of phenol polymers, notably black foods traditionally used in East Asia, but other non-edible, easily accessible sources, e.g., seaweeds and wood, have been considered with increasing interest together with waste materials from agro-based industries, primarily grape pomace and other byproducts of fruit and coffee processing. Not in all cases were the main structural components of these materials identified because of their highly heterogeneous nature. The great beneficial effects of natural phenol-based polymers on human health and their potential in improving the quality of food were largely explored, and this review critically addresses the most interesting and innovative reports in the field of nutrition and biomedicine that have appeared in the last five years. Several in vivo human and animal trials supported the proposed use of these materials as food supplements and for amelioration of the health and production of livestock. Biocompatible and stable functional polymers prepared by peroxidase-catalyzed polymerization of natural phenols, as well as natural phenol polymers were exploited as conventional and green plastic additives in smart packaging and food-spoilage prevention applications. The potential of natural phenol polymers in regenerative biomedicine as additives of biomaterials to promote growth and differentiation of osteoblasts is also discussed.

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Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-richEcklonia cavaextract Seanol^®to endow antibacterial properties and promote mineralization

Cited by 24 publications

References 38 publications

Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities

Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities

Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions

Natural Phenol Polymers: Recent Advances in Food and Health Applications

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Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-richEcklonia cavaextract Seanol®to endow antibacterial properties and promote mineralization

Cited by 24 publications

References 38 publications

Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities

Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities

Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions

Natural Phenol Polymers: Recent Advances in Food and Health Applications

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Product

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Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-richEcklonia cavaextract Seanol^®to endow antibacterial properties and promote mineralization