EntE, EntS and TolC synergistically contributed to the pathogenesis of APEC strain E058

Mu, Xin; Gao, Ruxia; Xiao, Wenhua; Gao, Qingqing; Cao, Chunguang; Xu, Huiqinq; Gao, Song; Liu, Xiufan

doi:10.1016/j.micpath.2020.103990

Cited by 10 publications

(6 citation statements)

References 56 publications

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“…Two chromosomal regions are homologous to two important siderophore biosynthetic regions that encode enterobactin and yersiniabactin. Ferric enterobactin is a catecholate siderophore common to pathogenic enterobacteria, and previous studies have shown that enterobactin is essential to the virulence of Salmonella Typhi and extraintestinal pathogenic E. coli (ExPEC) 55,56 . Yersiniabactin, a siderophore, can remove iron from a number of mammalian proteins based on its extremely high affinity for ferric iron 57 .…”

Section: Resultsmentioning

confidence: 99%

Genomic insights into evolution of pathogenicity and resistance of multidrug‐resistant Raoultella ornithinolytica WM1

Wang

Fan

Liu

et al. 2021

Annals of the New York Academy of Sciences

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Raoultella ornithinolytica is a poorly understood opportunistic pathogen, and the underlying mechanisms of its multidrug resistance and pathogenicity have not yet been comprehensively investigated. The multidrug‐resistant (MDR) strain WM1 was isolated from the blood of a male patient in Tianjin, China, in 2018. Here, we describe the complete genome and provide a genomic analysis of R. ornithinolytica WM1. The isolate was resistant to all tested antimicrobials except amikacin, tobramycin, and tigecycline. Two plasmids, pWM1‐1 (IncHI5) and pWM1‐2 (IncR), carried multidrug‐resistance regions. A large antimicrobial resistance island region resided on pWM1‐1 and exhibited mosaic structures resulting from the acquisition of complex integrations of variable regions, including genes conferring resistance to multiple classes of antimicrobials. Moreover, WM1 possessed virulence‐related elements that encode several virulence factors, including type I fimbriae, Escherichia coli common pilus, type II and VI secretion systems, yersiniabactin, enterobactin, and surface polysaccharide, indicating pathogenic potential. Furthermore, the core genome phylogeny and pan‐genome analyses revealed extensive genetic diversity. Our analysis indicates the need for stringent infection control, antimicrobial stewardship, periodic resistance monitoring, and rational medication to address potential threats posed by MDR R. ornithinolytica strains.

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Section: Resultsmentioning

confidence: 99%

Genomic insights into evolution of pathogenicity and resistance of multidrug‐resistant Raoultella ornithinolytica WM1

Wang

Fan

Liu

et al. 2021

Annals of the New York Academy of Sciences

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“…Thus, we compared the survival rate of the mutant and WT strains in whole mouse blood. The results of the whole-blood experiment showed that the ExPEC survival rate of the ΔTolC group was significantly reduced after 1 h. However, Mu et al ( 33 ) reported that the ΔTolC had similar survival rate to the wild-type strain in specific pathogen-free (SPF) chicken serum. The reason for this discrepancy might be due to the different dependence of serum resistance on TolC expression between mice and chicks.…”

Section: Discussionmentioning

confidence: 99%

“…In agreement with our results, Buckley et al ( 21 ) reported that disruption of TolC abolished the ability of S. Typhimurium to adhere, invade, and survive in both cell types. In addition, Mu et al ( 33 ) reported that disruption of TolC significantly decreased the pathogenicity of Avian pathogenic E. coli . This suggests that the absence of TolC may lead to fewer bacteria colonization in vivo and cause less tissue damage to the host post-infection.…”

Section: Discussionmentioning

confidence: 99%

Effects of TolC on the pathogenicity of porcine extraintestinal pathogenic Escherichia coli

Wang

Huang

et al. 2022

Front. Immunol.

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Extraintestinal pathogenic Escherichia coli (ExPEC) is a well-known critical pathogenic zoonosis that causes extraintestinal infections in humans and animals by affecting their immune organs. Recently, research on the outer membrane protein of E. coli, tolerant colicin (TolC), a virulent protein in the formation of the ExPEC efflux pump, has been an attractive subject. However, the pathogenic mechanisms remain unclear. This study aimed to explore the role of TolC in the pathogenesis of the ExPEC strain PPECC42; a complementation strain (Cm-TolC) and an isogenic mutant (ΔTolC) were constructed. Loss of TolC drastically impaired the virulence of ExPEC in an experimental mouse model. ΔTolC showed a substantial decrease in the porcine aortic vascular endothelial cell (PAVEC) adherence, invasion, and pro-inflammatory response, in contrast to that of the wild type, with a reduced survival ratio in both the bacterial load and whole blood in mice. ΔTolC also showed decreased expression of necroptosis signals such as receptor-interacting protein kinase 1, phosphorylated mixed-lineage kinase domain-like protein, and mitochondrial proteins such as phosphoglycerate mutase family member 5. Our data suggest that TolC is closely associated with ExPEC pathogenesis. These results provide scientific grounds for exploring the potential of TolC as an effective drug target for controlling ExPEC infection, screening new inhibitors, and developing new drugs. This will allow for further prevention and control of ExPEC infection.

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“…Anti-phagocytic function plays a significant role in the pathogenesis in extraintestinal infections. 35 Enhanced anti-phagocytic function might protect the E. coli ST1193 strains from phagocytosis and promote them survival in vivo which conduced to cause infections. In addition, capsule, lipopolysaccharide, mannose receptor, ClpX protease and carnitine metabolism have been demonstrated to mediate anti-phagocytosis in bacteria cells and phagocyte.…”

Section: Discussionmentioning

confidence: 99%

<p>A Comparative Study of Fluoroquinolone-Resistant Escherichia coli Lineages Portrays Indistinguishable Pathogenicity- and Survivability-Associated Phenotypic Characteristics Between ST1193 and ST131</p>

Huang¹,

Zhang²,

Zhang³

et al. 2020

IDR

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Background: Sequence type 1193 is a new such lineage among fluoroquinolone-resistant Escherichia coli, which has risen dramatically within the last several years. However, reasons for rapid emergence and successful spread of E. coli ST1193 remain unclear. The aim of this study was to compare the pathogenicity and survivability features of E. coli ST1193 with global epidemic lineage, ST131. Methods: A total of 30 E. coli were used in this study. Isolates were divided into two groups, ST1193 (n=15) and ST131 (n=15). Adhesion and invasion to T24 cells and resistance to serum were quantified and compared among two groups. Biofilm formation capacity was assessed by crystal violet assay. Macrocolony formation was assessed on macrocolony formation plates. Resistance to hydrogen peroxide was performed by broth microdilution. RAW264.7 cells were used to assess the anti-phagocytic function of different isolates. Results: Adhesion and invasion assays revealed that E. coli ST1193 could adhere and invade T24 cells (p <0.05). 93.3% of E. coli ST1193 could form biofilms. The majority of E. coli ST1193 (66.7%) possessed no curli/no cellulose on macrocolony formation plates. E. coli ST1193 showed significant growth in serum and hydrogen peroxide and illustrated higher anti-phagocytic function to RAW264.7 cells (p <0.05). Group analysis showed that E. coli ST1193 was similar to ST131 in pathogenicity-and survivability-associated phenotypic characteristics (p >0.05). Conclusion: Our study provided more insights into pathogenicity and survivability features of E. coli ST1193, which was similar to ST131. Our study could be of great importance in understanding the emergence of global spread E. coli ST1193. Strategic and continued surveillance should be carried out to prevent the infections caused by E. coli ST1193.

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EntE, EntS and TolC synergistically contributed to the pathogenesis of APEC strain E058

Cited by 10 publications

References 56 publications

Genomic insights into evolution of pathogenicity and resistance of multidrug‐resistant Raoultella ornithinolytica WM1

Genomic insights into evolution of pathogenicity and resistance of multidrug‐resistant Raoultella ornithinolytica WM1

Effects of TolC on the pathogenicity of porcine extraintestinal pathogenic Escherichia coli

<p>A Comparative Study of Fluoroquinolone-Resistant Escherichia coli Lineages Portrays Indistinguishable Pathogenicity- and Survivability-Associated Phenotypic Characteristics Between ST1193 and ST131</p>

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