Enteral feeding with low-methoxyl pectin accelerates colonic anastomosis healing in rats

Yamada, Fumiyo; Endo, Naoyuki; Miyatake, Sho; Ebisu, Goro; Hino, Kazuo

doi:10.1016/j.nut.2017.07.013

Cited by 10 publications

(6 citation statements)

References 21 publications

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“…Total short chain fatty acids (SCFAs) in cecal contents were higher in rats given EN with LM pectin than in rats given EN without pectin or HM pectin‐supplemented EN (Yamada et al. ). As SCFAs are known to enhance absorption of electrolytes and fluids from the colon, LM pectin might promote fecal bulking caused by colonic fermentation.…”

Section: Discussionmentioning

confidence: 99%

Fecal imaging demonstrates that low-methoxyl pectin supplementation normalizes gastro-intestinal transit in mice given a liquid diet

et al. 2018

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This study has the following aims: (1) to confirm a methodology for a fecal indocyanine green (ICG) imaging test for measuring gastro‐intestinal transit time (GITT); and (2) to compare GITT in mice given a liquid diet in which viscosity increases under acidic conditions to that in mice given stable liquid diets with comparable viscosity or regular chow. To address Aim 1, mice received ICG orally along with intraperitoneal injection of atropine in Study 1, and mice were given ICG orally with concurrent carmine red for Study 2. Fluorescence imaging of feces collected for 8 h thereafter was used to detect the first feces with fluorescence and thereby determine GITT. To address Aim 2, mice were fed ad libitum for 1 week with either liquid diet or regular chow for Study 3, or with liquid diet containing low‐methoxyl (LM) pectin or high‐methoxyl (HM) pectin, or regular chow for Study 4. GITT was then determined by fecal ICG imaging. Atropine delayed GITT in a dose‐dependent manner. The GITT of ICG completely corresponded to that of carmine red (correlation coefficient, 1.00). The first ICG excretion in the loose/some diarrheal feces of mice given a liquid diet was seen at 170 min. Feces of mice given liquid diet were loose with LM pectin and loose/some diarrhea with HM pectin. GITT of mice given liquid diet with HM pectin was significantly delayed (280 min) compared to that of mice given liquid diet with LM pectin (111 min) or regular chow (130 min). Fecal imaging of ICG enables measurements of GITT. LM pectin supplementation in a liquid diet may normalize GITT in mice to that of a normal meal and may be associated with changes in fecal properties.

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Section: Discussionmentioning

confidence: 99%

Fecal imaging demonstrates that low-methoxyl pectin supplementation normalizes gastro-intestinal transit in mice given a liquid diet

et al. 2018

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“…Pectins have α(1-4)-linked galacturonic acid backbone and can differ in degree of methyl esterification (DM). Dietary effects of pectin have been shown to be effective against endotoxin-induced inflammations ( 18 – 21 ) allergic airway inflammations ( 22 ), colitis ( 23 ), pancreatitis ( 24 ), and in supporting colonic anastomosis healing ( 25 ) in various animal models. Although anti-inflammatory effects of pectin are recognized, only a few studies have addressed mechanistic differences of pectins with different DM values ( 18 , 24 , 25 ) and none of the studies addressed true binding or identification of specific immune receptor responsible for the anti-inflammatory effects.…”

Section: Introductionmentioning

confidence: 99%

Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2–1 and Prevents Doxorubicin-Induced Ileitis

et al. 2018

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Dietary carbohydrate fibers are known to prevent immunological diseases common in Western countries such as allergy and asthma but the underlying mechanisms are largely unknown. Until now beneficial effects of dietary fibers are mainly attributed to fermentation products of the fibers such as anti-inflammatory short-chain fatty acids (SCFAs). Here, we found and present a new mechanism by which dietary fibers can be anti-inflammatory: a commonly consumed fiber, pectin, blocks innate immune receptors. We show that pectin binds and inhibits, toll-like receptor 2 (TLR2) and specifically inhibits the proinflammatory TLR2–TLR1 pathway while the tolerogenic TLR2–TLR6 pathway remains unaltered. This effect is most pronounced with pectins having a low degree of methyl esterification (DM). Low-DM pectin interacts with TLR2 through electrostatic forces between non-esterified galacturonic acids on the pectin and positive charges on the TLR2 ectodomain, as confirmed by testing pectin binding on mutated TLR2. The anti-inflammatory effect of low-DM pectins was first studied in human dendritic cells and mouse macrophages in vitro and was subsequently tested in vivo in TLR2-dependent ileitis in a mouse model. In these mice, ileitis was prevented by pectin administration. Protective effects were shown to be TLR2–TLR1 dependent and independent of the SCFAs produced by the gut microbiota. These data suggest that low-DM pectins as a source of dietary fiber can reduce inflammation through direct interaction with TLR2–TLR1 receptors.

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“…Many agents have been used to forestall adhesion formation by delimitating inflammatory reactions during peritoneal healing, thereby reducing the creation of a fibrinous network between serosal surfaces. [12][13][14]17,19,20] Although detecting the properties of KO that depress the formation of peritoneal adhesions may be an issue of another study, we think the administration of omega-3 sources (either KO or FO) decreases the inflammation on peritoneal surfaces in the critical stage of peritoneal wound healing.…”

Section: Discussionmentioning

confidence: 98%

“…Many investigators have researched on anastomotic wound healing process. [12,14,[17][18][19][20] And these studies also investigated the effectiveness of diverse systemic or local applied agents on the anastomotic healing process. [17,21,22] Inflammation during wound healing process stimulates the formation of fibrous bands between serosal surfaces ultimately.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of two different marine-derived omega- 3 fatty acid sources, krill oil and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model

Ferhatoğlu

Kıvılcım

Vural

et al. 2019

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: Oils from marine organisms have a different fatty acid composition. Fish oil (FO) has a high content of eicosapentaenoic and docosahexaenoic acids esterified to triacylglycerols; while in krill oil (KO), fatty acids are primarily esterified to phospholipids. This study aimed to compare the efficacy of two different, marine-derived omega-3 fatty acid sources in the wound healing of colon anastomoses rat model. METHODS: For the study, we used 42 male Wistar albino rats. The rats were divided into six groups with seven rats in each group-CO3: left colonic anastomosis (control group), sacrificed on the third day; KO3: left colonic anastomosis + oral KO, sacrificed on the third day; FO3: left colonic anastomosis + oral FO, sacrificed on the third day; CO7: left colonic anastomosis (control group), sacrificed on the seventh day; KO7: left colonic anastomosis + oral KO, sacrificed on the seventh day; FO7: left colonic anastomosis + oral FO, sacrificed on the seventh day. Peritoneal adhesions, anastomotic bursting pressures, hydroxyproline levels, and histological examination of the anastomotic tissue were evaluated. RESULTS: On day 7, bursting pressure and hydroxyproline measurements of the KO group was significantly higher than the FO group (p=0.012; p=0.002, respectively). Also, on day 7, a statistically significant difference was observed between the groups according to inflammatory cell infiltration, fibroblast activity, neoangiogenesis, and collagen deposition in favor of the KO group (p=0.023; p=0.028; p=0.016; p=0.012, respectively). CONCLUSION: Both KO and FO supplementation in patients before colorectal surgery may reduce some risk of anastomotic leakage; and KO might be a better alternative and excellent omega-3 source.

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Enteral feeding with low-methoxyl pectin accelerates colonic anastomosis healing in rats

Abstract: These results suggest that LMP promotes healing of colonic anastomosis more effectively than HMP, which may be explained by the mechanical stresses generated by the movement of normally formed stool though the colon.

Cited by 10 publications

References 21 publications

Fecal imaging demonstrates that low-methoxyl pectin supplementation normalizes gastro-intestinal transit in mice given a liquid diet

Fecal imaging demonstrates that low-methoxyl pectin supplementation normalizes gastro-intestinal transit in mice given a liquid diet

Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2–1 and Prevents Doxorubicin-Induced Ileitis

Comparison of the effects of two different marine-derived omega- 3 fatty acid sources, krill oil and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model

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