Environmental cue affects the hearing-related behaviors of Drosophila melanogaster by targeting the redox pathways

Dhar, Gyanaseni; Bag, Janmejaya; Mishra, Monalisa

doi:10.1007/s11356-020-09141-0

Cited by 4 publications

(7 citation statements)

References 104 publications

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“…The de‐esterified compound is altered to an exceedingly fluorescent DCF on oxidation of ROS. Upon excitation, at 488 nm, the fluorescence intensity is in proportion with the production of intracellular ROS (Dhar, Bag, et al, 2020). The DCF fluorescence signifies the accumulation of ROS within the cell which were quantified by flow cytometry (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

“…The production of ROS was determined by using a cell‐permeable fluorogenic dye 2′,7′‐dichlorofluorescein diacetate (DCF‐DA) using fluorescence‐activated cell sorting (FACS) by following Dhar, Bag, et al (2020) and Ng et al (2017). Briefly, antennae homogenate (100 flies, 3–4 days old) was prepared for each treatment group by adding 100 µl PBS.…”

Section: Methodsmentioning

confidence: 99%

“…Inter male aggression (3–8 days old) behavior was checked by following Dhar, Bag, et al (2020) and Versteven et al (2017). In the control, the total aggressive encounter was converted to a percentage and the aggressive encounter of each concentration of the three different types of antibiotics treated flies were normalized according to control (wild‐type).…”

Section: Methodsmentioning

confidence: 99%

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Aminoglycoside treatment alters hearing‐related genes and depicts behavioral defects in Drosophila

Dhar

Paikra

Mishra

2022

Arch Insect Biochem Physiol

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The hearing organ of Drosophila is present within the second segment of antennae. The hearing organ of Drosophila (Johnston's organ [JO]) shares much structural, developmental, and functional similarity with the vertebrate hearing organ (Organ of Corti). JO is evolving as a potential model system to examine the hearing‐associated defects in vertebrates. In the vertebrates, aminoglycosides like gentamicin, kanamycin, and neomycin have been known to cause defects in the hearing organ. However, a complete mechanism of toxicity is not known. Taking the evolutionary conservation into account the current study aims to test various concentrations of aminoglycoside on the model organism, Drosophila melanogaster. The current study uses the oral route to check the toxicity of various aminoglycosides at different concentrations (50, 100, 150, 200, and 250 μg ml−1). In Drosophila, many foreign particles enter the body through the gut via food. The aminoglycoside treated third instar larvae show defective crawling and sound avoidance behavior. The adult flies release lower amounts of acetylcholine esterase and higher amounts of reactive oxygen species than control untreated animals, accompanied by defective climbing and aggressive behavior. All these behavioral defects are further confirmed by the altered expression level of hearing genes such as nompC, inactive, nanchung, pyrexia. All the behavioral and genetic defects are reported as a readout of aminoglycoside toxicity.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Aminoglycoside treatment alters hearing‐related genes and depicts behavioral defects in Drosophila

Dhar

Paikra

Mishra

2022

Arch Insect Biochem Physiol

Self Cite

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“…Similarly, the larval body has numerous channels to detect the sound. To detect the sound sensing ability of the larva, sound avoidance behavioral assay is used in the third instar larvae (Dhar et al, 2020a(Dhar et al, , 2020b. If there is any defect in the channel, the larvae do not respond to the sound properly.…”

Section: Behavioral Assays Associated With Developmental Stagesmentioning

confidence: 99%

“…A defective mechanosensory organ resulted in faulty grooming behavior keeping the fly dirty (Sahu and Mishra, 2020a). Aggressive behavior helps the animal to find its food and partner, and protect the territory (Dhar et al, 2020a;Sahu et al, 2020). Aggressive behavior is associated with the environment, and (Dhar et al, 2020a) the level of serotonin and dopamine also regulates aggression (Alekseyenko et al, 2013).…”

Section: Complex Behaviorsmentioning

confidence: 99%

Drosophila melanogaster as an indispensable model to decipher the mode of action of neurotoxic compounds

Mishra¹,

PANDA²,

Barik³

et al. 2023

Biocell

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Exposure to some toxic compounds causes structural and behavioral anomalies associated with the neurons in the later stage of life. Those toxic compounds are termed as a neurotoxicant, which can be a physical factor, a toxin, an infection, radiation, or maybe a drug. The incongruities caused due to a neurotoxicant further depend on the toxicity of the compound. More importantly, the neurotoxicity of the compound is associated with the concentration and the time point of exposure. The neurodevelopmental defect appears depending on the toxicity of the compound. A neurodevelopmental defect may be associated with a delay in developmental time, defective growth, structural abnormality of many organs, including sensory organs, behavioral abnormalities, or death in the fetus stage. Numerous model organisms are employed to assess the effect of neurotoxicants. The current review summarizes several methods used to check the effect of neurotoxicant and their effect using the model organism Drosophila melanogaster.

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Platinum as a Novel Nanoparticle for Wound Healing Model in Drosophila melanogaster

et al. 2022

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Environmental cue affects the hearing-related behaviors of Drosophila melanogaster by targeting the redox pathways

Cited by 4 publications

References 104 publications

Aminoglycoside treatment alters hearing‐related genes and depicts behavioral defects in Drosophila

Aminoglycoside treatment alters hearing‐related genes and depicts behavioral defects in Drosophila

Drosophila melanogaster as an indispensable model to decipher the mode of action of neurotoxic compounds

Platinum as a Novel Nanoparticle for Wound Healing Model in Drosophila melanogaster

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