Enzymatic, antimicrobial, and leishmanicidal bioactivity of gram-negative bacteria strains from the midgut of Lutzomyia evansi, an insect vector of leishmaniasis in Colombia

Vivero, Rafael José; Mesa, Gustavo Bedoya; Robledo, Sara M.; Herrera, Claudia Ximena Moreno; Cadavid-Restrepo, Gloria

doi:10.1016/j.btre.2019.e00379

Cited by 13 publications

(8 citation statements)

References 67 publications

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“…b Differences in phyla composition (phylum level) in the foregut, midgut, and hindgut compartments (see Online Resource 4) pathogenicity cannot be inferred from taxonomy. The biotechnological applications of insect-derived Enterobacteriaceae include their ability to deliver toxic genes to pest insects (Zhang et al 2010), their use as a source of plant-stimulating (Pan et al 2019) and antimicrobial (Vivero et al 2019) metabolites, and their use as in insect-rearing facilities (Augustinos et al 2015;Azis et al 2019). Shimwellia blattae (synonym: Escherichia blattae) was originally isolated from Blatta orientalis (Burgess et al 1973) and later from other cockroaches (strains DSM 4481, ATCC 33429 and ATCC 33430), and interestingly, this species can synthesize cobalamin de novo and has been developed as a biotechnological source of vitamin B 12 (Andres et al 2004;Brzuszkiewicz et al 2012).…”

Section: Proteobacteriamentioning

confidence: 99%

Bacteria associated with cockroaches: health risk or biotechnological opportunity?

Guzman

Vilcinskas

2020

Appl Microbiol Biotechnol

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Cockroaches have existed for 300 million years and more than 4600 extant species have been described. Throughout their evolution, cockroaches have been associated with bacteria, and today Blattabacterium species flourish within specialized bacteriocytes, recycling nitrogen from host waste products. Cockroaches can disseminate potentially pathogenic bacteria via feces and other deposits, particularly members of the family Enterobacteriaceae, but also Staphylococcus and Mycobacterium species, and thus, they should be cleared from sites where hygiene is essential, such as hospitals and kitchens. On the other hand, cockroaches also carry bacteria that may produce metabolites or proteins with potential industrial applications. For example, an antibiotic-producing Streptomyces strain was isolated from the gut of the American cockroach Periplaneta americana. Other cockroach-associated bacteria, including but not limited to Bacillus, Enterococcus, and Pseudomonas species, can also produce bioactive metabolites that may be suitable for development as pharmaceuticals or plant protection products. Enzymes that degrade industrially relevant substrates, or that convert biomasses into useful chemical precursors, are also expressed in cockroach-derived bacteria and could be deployed for use in the food/feed, paper, oil, or cosmetics industries. The analysis of cockroach gut microbiomes has revealed a number of lesser-studied bacteria that may form the basis of novel taxonomic groups. Bacteria associated with cockroaches can therefore be dangerous or useful, and this review explores the bacterial clades that may provide opportunities for biotechnological exploitation. Key points • Members of the Enterobacteriaceae are the most frequently cultivated bacteria from cockroaches. • Cultivation-independent studies have revealed a diverse community, led by the phyla Bacteroidetes and Firmicutes. • Although cockroaches may carry pathogenic bacteria, most strains are innocuous and may be useful for biotechnological applications.

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Section: Proteobacteriamentioning

confidence: 99%

Bacteria associated with cockroaches: health risk or biotechnological opportunity?

Guzman

Vilcinskas

2020

Appl Microbiol Biotechnol

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“…Furthermore, our methodology does not allow to identify the exact section of the gut where T. cruzi is present, therefore, it remains a possibility that the parasite transiently affects the bacterial communities when it is located in certain regions of the gut. In the future, all this could be addressed by performing more precise dissections of the insects and mechanistic experiments in which the functionality of the bacteria is considered as the focus of the study, as has been proposed for Leishmania and Lutzomyia [42]. Additionally, geographic location was ultimately not included in the main analyses of our study given the differences in sample size, and this aspect should be improved in upcoming studies.…”

Section: Discussionmentioning

confidence: 99%

Species-dependent variation of the gut bacterial communities across Trypanosoma cruzi insect vectors

et al. 2020

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Triatomines (Hemiptera: Reduviidae) are the insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. The gut bacterial communities affect the development of T. cruzi inside the vector, making the characterization of its composition important in the understanding of infection development. We collected 54 triatomine bugs corresponding to four genera in different departments of Colombia. DNA extraction and PCR were performed to evaluate T. cruzi presence and to determine the discrete typing unit (DTU) of the parasite. PCR products of the bacterial 16S rRNA gene were pooled and sequenced. Resulting reads were denoised and QIIME 2 was used for the identification of amplicon sequence variants (ASVs). Diversity (alpha and beta diversity) and richness analyses, Circos plots, and principal component analysis (PCA) were also performed. The overall T. cruzi infection frequency was 75.9%, with TcI being the predominant DTU. Approximately 500,000 sequences were analyzed and 27 bacterial phyla were identified. The most abundant phyla were Proteobacteria (33.9%), Actinobacteria (32.4%), Firmicutes (19.6%), and Bacteroidetes (7.6%), which together accounted for over 90% of the gut communities identified in this study. Genera were identified for these main bacterial phyla, revealing the presence of important bacteria such as Rhodococcus, Serratia, and Wolbachia. The composition of bacterial phyla in the gut of the insects was significantly different between triatomine species, whereas no significant difference was seen between the state of T. cruzi infection. We suggest further investigation with the evaluation of additional variables and a larger sample size. To our knowledge, this study is the first characterization of the gut bacterial structure of the main triatomine genera in Colombia.

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“…Escherichia, Citrobacter, and Enterobacter are other genera within this order that are known to produce active lipopeptides, and they all have been identified as an active symbiont twice (Mandal et al 2013). Enterobacter, for example, has been reported as an active symbiont in the sand fly Lutzomya evansi and stag beetles of the Dorcus genus (Miyashita et al 2015;Vivero et al 2019).…”

Section: Antimicrobially Active Symbiont Strains Studied So Farmentioning

confidence: 99%

Microbial symbionts of insects as a source of new antimicrobials: a review

Moll

Smet

Cos

et al. 2021

Critical Reviews in Microbiology

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To stop the antimicrobial resistance crisis, there is an urgent need for increased investment in antimicrobial research and development. Currently, many researchers are focussing on insects and their microbiota in the search for new antimicrobials. This review summarizes recent literature dedicated to the antimicrobial screening of insect symbionts and/or their metabolites to uncover their value in early drug discovery. We summarize the main steps in the methodology used to isolate and identify active insect symbionts and have noted substantial variation among these studies. There is a clear trend in isolating insect Streptomyces bacteria, but a broad range of other symbionts has been found to be active as well. The microbiota of many insect genera and orders remains untargeted so far, which leaves much room for future research. The antimicrobial screening of insect symbionts has led to the discovery of a diverse array of new active biomolecules, mainly peptides, and polyketides. Here, we discuss 15 of these symbiont-produced compounds and their antimicrobial profile. Cyphomycin, isolated from a Streptomyces symbiont of a Cyphomyrmex fungus-growing ant, seems to be the most promising insect symbiont-derived antimicrobial so far. Overall, insect microbiota appears to be a promising search area to discover new antimicrobial drug candidates.

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Enzymatic, antimicrobial, and leishmanicidal bioactivity of gram-negative bacteria strains from the midgut of Lutzomyia evansi, an insect vector of leishmaniasis in Colombia

Cited by 13 publications

References 67 publications

Bacteria associated with cockroaches: health risk or biotechnological opportunity?

Bacteria associated with cockroaches: health risk or biotechnological opportunity?

Species-dependent variation of the gut bacterial communities across Trypanosoma cruzi insect vectors

Microbial symbionts of insects as a source of new antimicrobials: a review

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