Enzymatic methods of analysis: Novel approaches and applications

Abstract

The results of a series of investigations dealing with the development of enzymatic methods for determination of biologically active compounds, viz., inhibitors, activators, and substrates of native and immobilized enzymes of the oxidoreductase (peroxidases, alcohol dehydro genases) and hydrolase (alkaline and acid phosphatases) classes isolated from diverse sources are summarized. Novel original approaches, proposed by the authors, for improving the sensi tivity, selectivity, and rapidity of the methods are discussed. Numerous examples of application of the developed enzymatic procedures for the analysis of a wide range of samples are given.