Enzymatic Urea Sensor Based on Graphene Oxide/Titanium Dioxide Films Modified by Urease-Magnetic Beads

Chou, Jung-Chuan; Liao, Yi-Hung; Lai, Chih-Hsien; Yan, Siao-Jie; Wu, You-Xiang; Lin, Si-Hong

doi:10.1109/tnano.2019.2907225

Cited by 16 publications

(10 citation statements)

References 31 publications

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“…Comparing the results of the MBs-urease/GO/TiO 2 and MBs-urease/GO/NiO, it can be seen that the NiO-based matrix was more stable than the TiO 2 -based sensing matrix under dynamic detection. Under dynamic conditions, NiO promotes electrochemical reactions; this may be due to the high chemical stability of NiO [3,25,26].…”

Section: Resultsmentioning

confidence: 99%

“…The detailed steps for the fabrication of the biosensors can be found in our previous study [25,26], and the structure schematics are shown in Figure 2.…”

Section: Methodsmentioning

confidence: 99%

“…The structure schematics of (a) the MBs-urease/GO/TiO2-based biosensor [25] and (b) the MBs-urease/GO/NiO-based biosensor [26]. …”

Section: Figurementioning

confidence: 99%

“…Apart from this, TiO 2 or NiO are exceptional sensing matrices that can promote the conduction of interfacial electrons and are beneficial for binding the enzyme [24]. Therefore, this paper inherits the previous researches [25,26] and further explores the sensing characteristics, which the urea biosensors are integrated with the wireless measurement system and the microfluidic measurement system. Microfluidic systems are commonly used to analyze the concentration of the specific substance continuously, owing to the fast response time, the small uses of amounts of reagents, and automation [22,23].…”

Section: Introductionmentioning

confidence: 99%

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The Analysis of the Urea Biosensors Using Different Sensing Matrices via Wireless Measurement System & Microfluidic Measurement System

Chou

Lin

Kuo

et al. 2019

Sensors

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Two types of urea biosensors were integrated with a wireless measurement system and microfluidic measurement system. The two biosensors used were (i) a magnetic beads (MBs)-urease/graphene oxide (GO)/titanium dioxide (TiO2)-based biosensor and (ii) an MBs-urease/GO/ nickel oxide (NiO)-based biosensor, respectively. The wireless measurement system work exhibited the feasibility for the remote detection of urea, but it will require refinement and modification to improve stability and precision. The microchannel fluidic system showed the measurement reliability. The sensing properties of urea biosensors at different flow rates were investigated. From the measurement results, the decay of average sensitivity may be attributed to the induced vortex-induced vibrations (VIV) at the high flow rate. In the aspect of wireless monitoring, the average sensitivity of the urea biosensor based on MBs-urease/GO/NiO was 4.780 mV/(mg/dl) and with the linearity of 0.938. In the aspect of measurement under dynamic conditions, the average sensitivity of the urea biosensor based on MBs-urease/GO/NiO were 5.582 mV/(mg/dl) and with the linearity of 0.959. Both measurements performed NiO was better than TiO2 according to the comparisons.

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Section: Resultsmentioning

confidence: 99%

“…The detailed steps for the fabrication of the biosensors can be found in our previous study [25,26], and the structure schematics are shown in Figure 2.…”

Section: Methodsmentioning

confidence: 99%

“…The structure schematics of (a) the MBs-urease/GO/TiO2-based biosensor [25] and (b) the MBs-urease/GO/NiO-based biosensor [26]. …”

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Analysis of the Urea Biosensors Using Different Sensing Matrices via Wireless Measurement System & Microfluidic Measurement System

Chou

Lin

Kuo

et al. 2019

Sensors

Self Cite

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“…During long-term measurement, the response voltage of the sensor is changing with time, which is called the drift phenomenon. The reason that response voltage change is that hydration layer formed on the surface of sensing film [26,27]. The hydroxyl groups were formed on the surface of sensing film in solution, and the hydrated ions, formed by coulombic attraction between water molecules and ions, diffused to the sensing film will result in the formation of hydration layer.…”

Section: Introductionmentioning

confidence: 99%

A New Calibration Circuit Design to Reduce Drift Effect of RuO2 Urea Biosensors

Kuo

Dong

2019

Sensors

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The goal of this study was to reduce the drift effect of RuO2 urea biosensors. A new calibration circuit (NCC) based on the voltage regulation technique with the advantage of having a simple structure was presented. To keep its simplicity, the proposed NCC was composed of a non-inverting amplifier and a voltage calibrating circuit. A ruthenium oxide (RuO2) urea biosensor was fabricated to test the calibrating characteristics of the drift rate of the proposed NCC. The experiment performed in this study was divided into two main stages. For the first stage, a sound RuO2 urea biosensor testing environment was set-up. The RuO2 urea sensing film was immersed in the urea solution for 12 h and the response voltage was measured using the voltage-time (V–T) measurement system and the proposed NCC. The results of the first stage showed that the RuO2 urea biosensor has an average sensitivity of 1.860 mV/(mg/dL) and has a linearity of 0.999 which means that the RuO2 urea biosensor had been well fabricated. The second stage of the experiment verified the proposed NCC’s functions, and the results indicated that the proposed NCC reduced the drift rate of RuO2 urea biosensor to 0.02 mV/hr (98.77% reduction).

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NiCo2O4 Nano-/Microstructures as High-Performance Biosensors: A Review

Kumar¹

2020

Nano-Micro Lett.

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Non-enzymatic biosensors based on mixed transition metal oxides are deemed as the most promising devices due to their high sensitivity, selectivity, wide concentration range, low detection limits, and excellent recyclability. Spinel NiCo2O4 mixed oxides have drawn considerable attention recently due to their outstanding advantages including large specific surface area, high permeability, short electron, and ion diffusion pathways. Because of the rapid development of non-enzyme biosensors, the current state of methods for synthesis of pure and composite/hybrid NiCo2O4 materials and their subsequent electrochemical biosensing applications are systematically and comprehensively reviewed herein. Comparative analysis reveals better electrochemical sensing of bioanalytes by one-dimensional and two-dimensional NiCo2O4 nano-/microstructures than other morphologies. Better biosensing efficiency of NiCo2O4 as compared to corresponding individual metal oxides, viz. NiO and Co3O4, is attributed to the close intrinsic-state redox couples of Ni3+/Ni2+ (0.58 V/0.49 V) and Co3+/Co2+ (0.53 V/0.51 V). Biosensing performance of NiCo2O4 is also significantly improved by making the composites of NiCo2O4 with conducting carbonaceous materials like graphene, reduced graphene oxide, carbon nanotubes (single and multi-walled), carbon nanofibers; conducting polymers like polypyrrole (PPy), polyaniline (PANI); metal oxides NiO, Co3O4, SnO2, MnO2; and metals like Au, Pd, etc. Various factors affecting the morphologies and biosensing parameters of the nano-/micro-structured NiCo2O4 are also highlighted. Finally, some drawbacks and future perspectives related to this promising field are outlined.

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Enzymatic Urea Sensor Based on Graphene Oxide/Titanium Dioxide Films Modified by Urease-Magnetic Beads

Cited by 16 publications

References 31 publications

The Analysis of the Urea Biosensors Using Different Sensing Matrices via Wireless Measurement System & Microfluidic Measurement System

The Analysis of the Urea Biosensors Using Different Sensing Matrices via Wireless Measurement System & Microfluidic Measurement System

A New Calibration Circuit Design to Reduce Drift Effect of RuO2 Urea Biosensors

NiCo2O4 Nano-/Microstructures as High-Performance Biosensors: A Review

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