Enzyme-Assisted Oil Extraction of Lutein from Marigold (Tagetes erecta) Flowers and Stability of Lutein during Storage

Abstract

Marigold (Tagetes erecta) flowers are a rich source of organic solvents. In this study, lutein was extracted from marigold flowers with enzyme extraction without using organic solvents. Extraction produced oil that contained 0.36 mg/ml lutein (present as lutein esters but calculated as free lutein), and the yield was comparable to solvent extraction. The oil containing lutein was used to produce oil mixtures as stabilizers and some emulsions were also s containing lutein were stored in the dark at 20 monitored during storage. Stability of lutein was good in oil (85 emulsions (77-91%) but slightly worse in spray extraction of lutein from marigold flowers is a potential alternative to solvent extraction with comparable efficiency. In addition, there are no solvent residues in showed good stability of lutein in oil or in emulsions stored in the dark at room temperature, and these preparations could be used in different food products.

Analysis of Lutein Content in Microencapsulated Marigold Flower Extract (Tagetes erecta L.) Using UHPLC-Q-Orbitrap-HRMS and Its Cytotoxicity in ARPE-19 Cells

“…Lutein is normally extracted from marigold flowers via different kinds of organic solvents such as hexane, acetone, and ethanol, but the most popular and effective solvents are toxic. The organic solvent has to be removed completely via evaporation to ensure safety in food applications [ 11 ]. Therefore, an alternative extraction method—especially green extraction, which is environmental-friendly—needs to be discovered.…”

Stabilisation of Lutein and Lutein Esters with Polyoxyethylene Sorbitan Monooleate, Medium-Chain Triglyceride Oil and Lecithin

“…Marigold (Tagetes erecta) flowers contain high amounts of carotenoids, which are predominantly fatty esters and diesters of lutein (0.1-0.2% [w/w] dry matter as carotenoids, of which 80% are lutein diesters) (Figure 1B) [9]. In the food industry, lutein is usually extracted from marigold flowers either as its fatty acid esters and diesters (e.g., palmitate, myristate linoleate, laureate) or as free lutein when the extraction is combined with saponification ( [4,[10][11][12][13]. Furthermore, lutein esters are less sensitive to degradation than lutein, with its diesters being more stable than its monoesters [4].…”