Enzyme histochemical analysis of cell viability after argon laser-induced coagulation necrosis of the skin

Neumann, R.; Knobler, Robert; Pieczkowski, Friederike; Gebhart, W.

doi:10.1016/0190-9622(91)70296-e

Cited by 106 publications

(64 citation statements)

References 38 publications

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“…The tissue response characterized by thermal coagulation change, achieved by IUS exposure is similar to that from other energy-based devices used in the cosmetic arena such as lasers, radiofrequency (RF) and combination laser-RF devices [9,10,19]. However, in contrast to the other known energy based devices used for cosmetic applications, the IUS field is sharply focused, thereby depositing most of the energy in the form of heat around the focal zone of the beam, leaving the surrounding regions unaffected [3,4].…”

Section: Discussionmentioning

confidence: 66%

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Selective transcutaneous delivery of energy to porcine soft tissues using intense ultrasound (IUS)

White

Makin²,

Slayton³

et al. 2008

Lasers Surg Med

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Objective: Various energy delivery systems have been utilized to treat superficial rhytids in the aging face. The Intense Ultrasound System (IUS) is a novel modality capable of transcutaneously delivering controlled thermal energy at various depths while sparing the overlying tissues. The purpose of this feasibility study was to evaluate the response of porcine tissues to various IUS energy source conditions. Further evaluation was performed of the built-in imaging capabilities of the device. Materials and Methods: Simulations were performed on ex vivo porcine tissues to estimate the thermal dose distribution in tissues after IUS exposures to determine the unique source settings that would produce thermal injury zones (TIZs) at given depths. Exposures were performed at escalating power settings and different exposure times (in the range of 1-7.6 J) using three IUS handpieces with unique frequencies and focal depths. Ultrasound imaging was performed before and after IUS exposures to detect changes in tissue consistency. Porcine tissues were examined using nitro-blue tetrazolium chloride (NBTC) staining sensitive for thermal lesions, both grossly and histologically. The dimensions and depth of the TIZs were measured from digital photographs and compared. Results: IUS can reliably achieve discrete, TIZ at various depths within tissue without surface disruption. Changes in the TIZ dimensions and shape were observed as source settings were varied. As the source energy was increased, the thermal lesions became larger by growing proximally towards the tissue surface. Maximum lesion depth closely approximated the pre-set focal depth of a given handpiece. Ultrasound imaging detected well-demarcated TIZ at depths within the porcine muscle tissue. Conclusion: This study demonstrates the response of porcine tissue to various energy dose levels of Intense Ultrasound. Further study, especially on human facial tissue, is necessary in order to understand the utility of this modality in treating the aging face and potentially, other cosmetic applications.

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Section: Discussionmentioning

confidence: 66%

“…This method has been described in the literature for identifying thermally affected tissue both in vivo as well as for ex vivo studies [19][20][21]. Viable tissue stains blue and TIZs are demarcated by a pale color or lack of blue staining.…”

Section: Ius Exposure Proceduresmentioning

confidence: 99%

Selective transcutaneous delivery of energy to porcine soft tissues using intense ultrasound (IUS)

White

Makin²,

Slayton³

et al. 2008

Lasers Surg Med

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“…The plaque segments (n=5) were frozen before or 6 h after incubation, and then 5-μm sections were incubated at 37°C for 1 h in 10 ml of Gomeri Tris-HCl buffer (pH 7.4) containing 24 mg of nitroblue tetrazolium (Nacalai Tesque, Kyoto, Japan) and 10 mg of NADH (CalBiochem, San Diego, CA, USA). 19 The amount of TF protein in plaque segments was measured using a rabbit TF ELISA kit (Uscn Life Science Inc, Wuhan, China) before and at 6 h after incubation with Bay 11-7085 (an inhibitor of inhibitor of κB kinase, Merck, Darmstadt, Germany) or dimethyl sulfoxide.…”

Section: Atherosclerosis and Thrombosis Modelmentioning

confidence: 99%

Arterial <sup>18</sup>F-Fluorodeoxyglucose Uptake Reflects Balloon Catheter-Induced Thrombus Formation and Tissue Factor Expression via Nuclear Factor-κB in Rabbit Atherosclerotic Lesions

Yamashita

Zhao

Matsuura

et al. 2013

Circ J

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“…We and other authors [1][2][3][4][5][6][7][8] have used enzyme histochemistry to evaluate thermal damage immediately following irradiation. Thermal damage to cells results from the denaturation of biomolecules.…”

Section: Discussionmentioning

confidence: 99%

“…In order to limit the number of animals used in experiments and to increase the speed at which experiments can be completed, there is a need for methods that do not require the survival of the animals after the treatment. For laser-induced thermal damage, we and others [1][2][3][4][5][6][7][8] have used enzyme histochemical stains of tissue as an immediate method to assay for cell viability in tissue. This method shows decreased to absent staining when there has been thermal denaturation of many globular proteins.…”

Section: Introductionmentioning

confidence: 99%

Improved staining method for determining the extent of thermal damage to cells

Sherwood

Flotte

2006

Lasers Surg Med

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Background and Objective: Enzyme histochemical stains of frozen sections have been used by investigators to assess thermal damage. The assessment of thermal damage to cells in lipid-rich tissues such as subcutaneous tissue and sebaceous glands can be difficult due to the quality of frozen sections of such tissues. The purpose of this study is to develop an improved method for this type of evaluation. Study Design/Materials and Methods: Thick frozen sections of thermally damaged pig and human skin were stained for lactate dehydrogenase. The sections were fixed in formalin and processed for paraffin-embedded sections. Results: The sections showed well-defined localization of the enzymatic deposits as well as preservation of the tissue architecture. Conclusion: The paraffin-embedded lactate dehydrogenase stained sections provide improved evaluation of thermally damaged tissues, particularly the lipid rich tissues.

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Enzyme histochemical analysis of cell viability after argon laser-induced coagulation necrosis of the skin

Cited by 106 publications

References 38 publications

Selective transcutaneous delivery of energy to porcine soft tissues using intense ultrasound (IUS)

Selective transcutaneous delivery of energy to porcine soft tissues using intense ultrasound (IUS)

Arterial <sup>18</sup>F-Fluorodeoxyglucose Uptake Reflects Balloon Catheter-Induced Thrombus Formation and Tissue Factor Expression via Nuclear Factor-κB in Rabbit Atherosclerotic Lesions

Improved staining method for determining the extent of thermal damage to cells

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