Enzyme immobilisation on planar and porous silicon substrates for biosensor applications

Thust, M.; Schöning, Michael J.; Schroth, P.; Malkoc, Ü.; Dicker, C.I.; Steffen, Alfred; Kordoš, P.; Lüth, H.

doi:10.1016/s1381-1177(99)00023-5

Cited by 49 publications

(26 citation statements)

References 12 publications

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“…In 1996, a PSi layer was added at the surface of www.intechopen.com New Concepts of Integrated Photonic Biosensors Based on Porous Silicon 267 the bulk Si potentiometric device to measure penicillin concentration. An enlarged linear range of the calibration curve and higher signals were obtained, which were attributed to the larger amount of enzyme immobilised on the PSi substrate compared to planar structures (Thust et al, 1996(Thust et al, , 1999. To conclude, in the case of electrical transducers, the improvement of biosensor performances achieved with PSi layers is usually only due to an increase of the specific working area and not to improvement of the electrical properties.…”

Section: Biosensors Based On Porous Siliconmentioning

confidence: 92%

New concepts of integrated photonic biosensors based on porous silicon

Li¹,

Gerelli²,

Orobtchouk³

et al. 2011

Biosensors - Emerging Materials and Applications

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Section: Biosensors Based On Porous Siliconmentioning

confidence: 92%

New concepts of integrated photonic biosensors based on porous silicon

Li¹,

Gerelli²,

Orobtchouk³

et al. 2011

Biosensors - Emerging Materials and Applications

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“…This may involve nonspecific adsorption or biospecific adsorption of a ligand to a surface. Immobilization by nonspecific adsorption has been used for a long time [33][34][35][36] and can be performed with biopolymers like polysaccharides or proteins (e. g., collagen) as well as with inorganic materials like alumina and silica [37][38][39][40]. Although easy to perform, this approach can have problems with ligand stability and nonspecific binding of other compounds to the affinity support.…”

Section: Adsorption-based Immobilization Methodsmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

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The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.

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“…Sigma) as an enzyme sensitive to penicillin with a specific activity of 2660 units per mg protein was adsorptively immobilised into the pores. A detailed description of the procedure and the immobilisation conditions are given in [10].…”

Section: Methodsmentioning

confidence: 99%

A (Bio-)Chemical Field-Effect Sensor with Macroporous Si as Substrate Material and a SiO2 / LPCVD-Si3N4 Double Layer as pH Transducer

Schöning

Simonis

Ruge

et al. 2002

Sensors

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Macroporous silicon has been etched from n-type Si, using a vertical etching cell where no rear side contact on the silicon wafer is necessary. The resulting macropores have been characterised by means of Scanning Electron Microscopy (SEM). After etching, SiO 2 was thermally grown on the top of the porous silicon as an insulating layer and Si 3 N 4 was deposited by means of Low Pressure Chemical Vapour Deposition (LPCVD) as transducer material to fabricate a capacitive pH sensor. In order to prepare porous biosensors, the enzyme penicillinase has been additionally immobilised inside the porous structure. Electrochemical measurements of the pH sensor and the biosensor with an Electrolyte/Insulator/Semiconductor (EIS) structure have been performed in the Capacitance/Voltage (C/V) and Constant capacitance (ConCap) mode.

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Enzyme immobilisation on planar and porous silicon substrates for biosensor applications

Cited by 49 publications

References 12 publications

New concepts of integrated photonic biosensors based on porous silicon

New concepts of integrated photonic biosensors based on porous silicon

Applications of silica supports in affinity chromatography

A (Bio-)Chemical Field-Effect Sensor with Macroporous Si as Substrate Material and a SiO2 / LPCVD-Si3N4 Double Layer as pH Transducer

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