Enzyme profiling of lignocellulolytic fungi

Acharya, Dhaval; Chabhadiya, S; Shah, Amita; Shilpkar, Prateek; Acharya, P. B.; Modi, H. A.

doi:10.4314/ijbcs.v4i2.58148

Cited by 4 publications

(3 citation statements)

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“…A. oryzae is responsible for the secretion of the enzymes (primarily α -amylases and endo-1,4-α -D-glucan glucohydrolase EC 3.2.1.1) that hydrolyse the starch into glucose, which S. cerevisiae then utilises for ethanol production [10]. However A. oryzae has also been shown to secrete lignocellulolytic enzymes (endocellulases and various xylanases) in significant quantities when cultured in media containing lignocellulosic biomass [11]. The sake fermentation system was thus of interest to us as a potential CBP approach for lignocellulosic bioethanol production, particularly since A. oryzae and S. cerevisiae are known to exhibit suitably high ethanol tolerance phenotypes [12].…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

et al. 2016

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Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzymes required to deconstruct biomass, paired with a secondary yeast species to ferment liberated sugars to ethanol. Interestingly, although several pairings of fungi were investigated, the sake fermentation system (A. oryzae and S. cerevisiae NCYC479) was found to yield the highest concentrations of ethanol (37 g/L of ethanol within 10 days). On this basis, 1 t of BSG (dry weight) would yield 94 kg of ethanol using 36 hL of water in the process. QRT-PCR analysis of selected carbohydrate degrading (CAZy) genes expressed by A. oryzae in the BSG sake system showed that hemicellulose was deconstructed first, followed by cellulose. One drawback of the CBP approach is lower ethanol productivity rates; however, it requires low energy and water inputs, and hence is worthy of further investigation and optimisation.

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Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

et al. 2016

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“…Some known fungi are Trichoderma reesei, Pleurotus ostreatus, Tremetes versicolor, Neurospora crassa, Aspergillus oryzae, etc. [10][11][12]. These filamentous fungi secrete different enzymes depending on the morphological features of the microorganism and environmental factors.…”

Section: Introductionmentioning

confidence: 99%

Screening of Lignocellulolytic Enzyme Activities in Fungal Species and Sequential Solid-State and Submerged Cultivation for the Production of Enzyme Cocktails

et al. 2021

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Various fungal species can degrade lignocellulolytic materials with their enzyme cocktails composed of cellulolytic and lignolytic enzymes. In this work, seven fungal species (Mucor indicus DSM 2185, Paecilomyces variotii CBS 372.70, Myceliophthora thermophila CBS 663.74, Thielavia terrestris CBS 456.75, Botryosphaeria dothidea JCM 2738, Fusarium oxysporum f.sp. langenariae JCM 9293, and Fusarium verticillioides JCM 23107) and four nutrient media were used in the screening for effective lignocellulose degrading enzymes. From the seven tested fungi, F. oxysporum and F. verticilliodes, along with nutrient medium 4, were selected as the best medium and producers of lignocellulolytic enzymes based on the determined xylanase (>4 U mg−1) and glucanase activity (≈2 U mg−1). Nutrient medium 4 supplemented with pretreated corn cobs was used in the production of lignocellulolytic enzymes by sequential solid-state and submerged cultivation of F. oxysporum, F. verticilliodes, and a mixed culture of both strains. F. oxysporum showed 6 times higher exoglucanase activity (3.33 U mg−1) after 5 days of cultivation in comparison with F. verticillioides (0.55 U mg−1). F. oxysporum also showed 2 times more endoglucanase activity (0.33 U mg−1). The mixed culture cultivation showed similar endo- and exoglucanase activities compared to F. oxysporum (0.35 U mg−1; 7.84 U mg−1). Maximum xylanase activity was achieved after 7 days of cultivation of F. verticilliodes (≈16 U mg−1), while F. oxysporum showed maximum activity after 9 days that was around 2 times lower compared to that of F. verticilliodes. The mixed culture achieved maximum xylanase activity after only 4 days, but the specific activity was similar to activities observed for F. oxysporum. It can be concluded that both fungal strains can be used as producers of enzyme cocktails for the degradation of lignocellulose containing raw materials, and that corn cobs can be used as an inducer for enzyme production.

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“…Lignocellulosic biomass is a promising renewable resource for attaining value added products. (Acharya et al, 2010). Many microorganisms are capable of producing a variety of lignocellulolytic enzymes i.e.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Process Parameters for Enzymes Production From Trichoderma Sp. Sh2 Using Pine Needles Biomass Under Solid State Fermentation

Tanwar¹,

Sharma²,

Sharma³

2020

JBINO

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Enzyme profiling of lignocellulolytic fungi

Cited by 4 publications

References 30 publications

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

Screening of Lignocellulolytic Enzyme Activities in Fungal Species and Sequential Solid-State and Submerged Cultivation for the Production of Enzyme Cocktails

Evaluation of Process Parameters for Enzymes Production From Trichoderma Sp. Sh2 Using Pine Needles Biomass Under Solid State Fermentation

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