Enzymes and Isozymes in Cancer

Balinsky, Doris

doi:10.1007/978-1-4612-6117-9_8

Cited by 5 publications

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“…It could also explain conflicting results between laboratories (Kikuchi etal., 1972, Marshall etal., 1979. This may be relevant to studies where HK I1 did not consistently appear (see Balinsky, 1980). The binding of HK I to mitochondria of the human breast cancer is consistent with the observations in murine mammary glands by Bartley et al (1966Bartley et al ( ,1975.…”

Section: Discussionsupporting

confidence: 82%

“…It has been suggested that the duplication of HK I in fetal tissue is a secondary modification of the primary enzyme, similar to that observed for some enzymes in rapidly proliferating cells (Rogers et al, 1975). Rapid cell proliferation is common to both cancer and fetal tissue, and it has been suggested that the fetal stage tends to be reproduced in cancer (Balinsky, 1980). Bennett et al (1978), working on gliomas, observed HK I1 in neoplasia of high malignancy and a low grade of differentiation, and suggested that it was a consequence rather than the cause of neoplasia.…”

Section: Discussionmentioning

confidence: 94%

“…Samples were washed as free of blood as POShuman malignancies (Kame1 and Schwarzfisher, 1975) sible with ice-cold saline before and after mincing. and though it is not consistently found in all human 'hmors were either quick-frozen in co2 and kept at malignancies of the same tissue origin, there is a defi--70" c Until use, or, if used fresh, immediately nite trend in that direction (for a review, see Balinsky, homogenized and centrifuged to Yield isolated frat-1980 , , Balinsky, 1980. same way as tumor samples.…”

mentioning

confidence: 99%

“…More recent studies support this view (Kraai-tions. jenhagen et al, 1980;Beemer et al, 1982;Balinsky et Seven non-pathological samples were obtained from al., 1983); only the last study deals with human breast breasts removed by radical mastectomy. Fat was excancer.…”

mentioning

confidence: 99%

“…Samples used contained up to approximately tissue of human origin has also been observed (Rogers 30 % fat. Non-pathological samples were treated in the et al, , Balinsky, 1980. same way as tumor samples.…”

mentioning

confidence: 99%

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Isoenzyme pattern and subcellular localization of hexokinases in human breast cancer and nonpathological breast tissue

Gudnason¹,

Ingvarsson²,

Jónasdóttir³

et al. 1984

Intl Journal of Cancer

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Subcellular distribution of hexokinase (HK) isoenzymes in 22 human breast cancers (21 primary cancers and 1 axillary metastatic growth) and 7 non-pathological human mammary gland tissue samples was studied with starch gel electrophoresis on isolated cell fractions obtained by differential centrifugation. Fractions used were cytosol, mitochondria and microsomes. A comparison of two methods for detecting HK activity was made, yielding different results regarding HK II and HK III. A method based on the ability of NADPH to fluoresce in UV gave a constant pattern of HK isoenzymes. In non-pathological breast tissue, only HK I was seen, i.e. in the cytosol and the microsomal fractions. HK I was also seen in all fractions of the cancers, but another more anodal band of HK I, as well as HK II and HK III, consistently appeared in the cytosol fractions. The more commonly used staining technique with tetrazolium dye revealed HK II in 45% and HK III in 50% of the samples. The pattern of HK isoenzymes in the cancers was the same irrespective of estrogen and progesterone cytosolic receptor contents and the histology of the tumors. The fluorescence method is, therefore, much more sensitive than the tetrazolium technique for detecting HK activity after electrophoresis and could explain difference in results obtained by various laboratories.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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