Epidemiological analysis of <i>Mycobacterium tuberculosis</i> strains isolated in Lodz, Poland

Krawczyk, Michał; Brzostek, Anna; Górna, Alina; Knapska, Katarzyna; Ziolkiewicz, M.; Wojtasik, Arkadiusz; Dziadek, Jarosław

doi:10.5588/ijtld.10.0718

Cited by 14 publications

(19 citation statements)

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“…In this context, we extend our earlier, preliminary observations [19, 20] and present here the results on genotyping of 155 clinical M. tuberculosis isolates using FLiP in comparison with previously performed 15-locus MIRU-VNTR typing [21]. Clusters identified in both PCR-based methods were further analyzed by the reference IS 6110 RFLP.…”

Section: Introductionsupporting

confidence: 66%

Genotyping of ClinicalMycobacterium tuberculosisIsolates Based on IS6110and MIRU-VNTR Polymorphisms

Żaczek

Brzostek

Wojtasik

et al. 2013

BioMed Research International

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In this study, 155 clinical Mycobacterium tuberculosis isolates were subject to genotyping with fast ligation-mediated PCR (FLiP). This typing method is a modified mixed-linker PCR, a rapid approach based on the PCR amplification of HhaI restriction fragments of genomic DNA containing the 3′ end of IS6110 and resolving the amplicons by polyacrylamide gel electrophoresis. The results were compared with previous data of the more commonly used methods, 15-locus mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing and, to verify combined FLiP/MIRU-VNTR clusters, the reference IS6110 restriction fragment length polymorphism (RFLP). FLiP banding patterns were highly reproducible and polymorphic. This method differentiated 119 types among the study set compared to 108 distinct MIRU-VNTR profiles. The discriminatory power of FLiP was slightly higher than that of MIRU-VNTR analysis (Hunter-Gaston Discriminatory Index = 0.991 and 0.990, resp.). Detailed comparison of the clusters defined by each of the methods revealed, however, a more apparent difference in the discriminatory abilities that favored FLiP. Clustering of strains by using combined results of these two PCR-based methods correlated well with IS6110 RFLP-defined clusters, further confirming high discriminatory potential of FLiP typing. These results indicate that FLiP could be an attractive and valuable secondary typing technique for verification of MIRU-VNTR clusters of M. tuberculosis strains.

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Section: Introductionsupporting

confidence: 66%

Genotyping of ClinicalMycobacterium tuberculosisIsolates Based on IS6110and MIRU-VNTR Polymorphisms

Żaczek

Brzostek

Wojtasik

et al. 2013

BioMed Research International

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“…Additionally, convergent deletions in the CRISPR locus occur frequently (340). Spoligotyping, when used alone, is not sufficient for epidemiological studies, but it is sometimes recommended as a first-line screening test, especially when a large collection of isolates is being tested (331,341,342).…”

Section: Methods Based On Repetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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“…Previously performed analysis by reference methods, IS 6110 -RFLP and MIRU-VNTR typing, grouped this set of strains into 36, for IS 6110 -RFLP, and 27 patterns, for MIRU-VNTR, and gave the resolving power, 0.9743 and 0.9697, respectively [16]. …”

Section: Resultsmentioning

confidence: 99%

“…All strains were tested for susceptibility to isoniazid, rifampicin, pyrazinamide, streptomycin, and ethambutol using the Bactec 460 TB system (BD Diagnostic Systems, Sparks, MD, USA), as described previously [16]. This set of strains was previously characterized by IS 6110 -RFLP analysis, 15 locus MIRU-VNTR typing, and spoligotyping [16]. …”

Section: Methodsmentioning

confidence: 99%

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Comparison of Ligation-Mediated PCR Methods in Differentiation ofMycobacterium tuberculosisStrains

Żaczek

Brzostek

Wojtasik

et al. 2014

BioMed Research International

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Fast and inexpensive identification of epidemiological links between limited number of Mycobacterium tuberculosis strains is required to initially evaluate hospital outbreaks, laboratory crosscontaminations, and family or small community transmissions. The ligation-mediated PCR methods (LM-PCR) appear sufficiently discriminative and reproducible to be considered as a good candidate for such initial, epidemiological analysis. Here, we compared the discriminative power of the recently developed in our laboratory fast ligation amplification polymorphism (FLAP) method with fast ligation-mediated PCR (FLiP). Verification of the results was based on analyzing a set of reference strains and RFLP-IS6110 typing. The HGDI value was very similar for both LM-PCR methods and RFLP-IS6110 typing. However, only 52% of strains were correspondingly grouped by both FLiP and FLAP methods. Differentiation by FLAP method demonstrated a limited similarity to IS6110-RFLP (37,7%). As much as 78,7% of strains were grouped identically when differentiated by FLiP and IS6110-RFLP methods. The analysis differentiated 31, 35, and 36 groups when using FLAP, FLiP, and RFLP-IS6110 methods, respectively.

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Epidemiological analysis of Mycobacterium tuberculosis strains isolated in Lodz, Poland

Abstract: The combination of 15-loci MIRU-VNTR typing with spoligotyping is useful for primary analysis of M. tuberculosis strains; however, additional use of MIRU 23 should be considered. Strains clustered by PCR-based methods should be further analysed by IS6110-RFLP typing.

Cited by 14 publications

References 0 publications

Genotyping of ClinicalMycobacterium tuberculosisIsolates Based on IS6110and MIRU-VNTR Polymorphisms

Genotyping of ClinicalMycobacterium tuberculosisIsolates Based on IS6110and MIRU-VNTR Polymorphisms

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Comparison of Ligation-Mediated PCR Methods in Differentiation ofMycobacterium tuberculosisStrains

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