Epidermal growth factor receptor is required for estradiol-stimulated bovine satellite cell proliferation

Reiter, Brenda C.; Kamanga-Sollo, E.; Pampusch, Mary S.; White, Michael E.; Dayton, William R

doi:10.1016/j.domaniend.2014.01.001

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…Both proliferation of satellite cells and protein synthesis and degradation in developing muscle fibers play crucial roles in muscle growth and development in beef cattle [24]. Our previous studies [25,26] and results of the current study indicate that active GPER-1, MMP2/9 and hbEGF are required to support E2-mediated increases in both proliferation of mononucleated satellite cells and protein synthesis rate in fused BSC, while E2-mediated decreases in protein degradation rate in fused BSC does not require activity of these factors. We believe it is significant that E2 regulation of two dissimilar processes such as proliferation rate in unfused proliferating satellite cells and protein synthesis rate in fused BSC requires active GPER-1, MMP2/9 and hbEGF while the effect of E2 on protein degradation rate in fused BSC does not require these factors.…”

Section: Discussionmentioning

confidence: 97%

Role of G protein-coupled estrogen receptor-1 in estradiol 17β-induced alterations in protein synthesis and protein degradation rates in fused bovine satellite cell cultures

Kamanga-Sollo

Thornton

White

et al. 2017

Domestic Animal Endocrinology

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In feedlot steers, estradiol-17β (E2) and combined E2 and trenbolone acetate (a testosterone analog) implants enhance rate and efficiency of muscle growth; and, consequently, these compounds are widely used as growth promoters in several countries. Treatment with E2 stimulates protein synthesis rate and suppresses protein degradation rate in fused bovine satellite cell (BSC) cultures; however, the mechanisms involved in these effects are not known with certainty. Although the genomic effects of E2 mediated through the classical estrogen receptors have been characterized, recent studies indicate that binding of E2 to the G protein-coupled estrogen receptor (GPER)-1 mediates nongenomic effects of E2 on cellular function. Our current data show that inhibition of GPER-1, matrix metalloproteinases 2 and 9 (MMP2/9), or heparin binding epidermal growth factor-like growth factor (hbEGF) suppresses E2 stimulate protein synthesis rate in cultured BSCs (P < 0.001) suggesting that all of these are required in order for E2 to stimulate protein synthesis in these cultures. In contrast, inhibition of GPER-1, MMP2/9, or hbEGF has no effect on the ability of E2 to suppress protein degradation rates in fused BSC cultures indicating that these factors are not required in order for E2 to suppress protein degradation rate in these cells. Furthermore, treatment of fused BSC cultures with E2 increased (P < 0.05) pAKT levels indicating that the pAKT pathway may play a role in E2-stimulated effects on cultured BSC. In summary, our current data show that active GPER-1, MMP2/9, and hbEGF are necessary for E2-stimulated protein synthesis but not for E2-simulated suppression of protein degradation in cultured BSC. In addition, E2 treatment increases pAKT levels in cultured BSC.

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Section: Discussionmentioning

confidence: 97%

Role of G protein-coupled estrogen receptor-1 in estradiol 17β-induced alterations in protein synthesis and protein degradation rates in fused bovine satellite cell cultures

Kamanga-Sollo

Thornton

White

et al. 2017

Domestic Animal Endocrinology

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“…Moreover, decrease in the level of sex hormones with age is responsible for the development of sarcopenia in 30% of individuals aged over 60 years ( 35 , 36 ). Furthermore, results obtained from rodent models demonstrated that androgens and estrogens augment skeletal muscle satellite stem cell activation and modulate inflammatory processes during skeletal muscle regeneration ( 9 , 12 ). Despite the fact that gonadal sex hormones affect expansion of skeletal muscle cells, very little is known about the role of gonadal and pituitary sex hormones in RMS pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…First, it is well known that these hormones are involved in both developmental growth and regeneration of skeletal muscles ( 9 – 11 ). For example, skeletal muscle satellite stem cells respond to stimulation by estrogens and androgens ( 9 , 12 ). Second, pediatric sarcomas, including RMS, share several common markers with the germ line-derived cells.…”

Section: Introductionmentioning

confidence: 99%

Human rhabdomyosarcoma cells express functional pituitary and gonadal sex hormone receptors: Therapeutic implications

Poniewierska-Baran

Schneider

Sun

et al. 2016

International Journal of Oncology

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Evidence has accumulated that sex hormones play an important role in several types of cancer. Because they are also involved in skeletal muscle development and regeneration, we were therefore interested in their potential involvement in the pathogenesis of human rhabdomyosarcoma (RMS), a skeletal muscle tumor. In the present study, we employed eight RMS cell lines (three fusion positive and five fusion negative RMS cell lines) and mRNA samples obtained from RMS patients. The expression of sex hormone receptors was evaluated by RT-PCR and their functionality by chemotaxis, adhesion and direct cell proliferation assays. We report here for the first time that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) receptors are expressed in established human RMS cell lines as well as in primary tumor samples isolated from RMS patients. We also report that human RMS cell lines responded both to pituitary and gonadal sex hormone stimulation by enhanced proliferation, chemotaxis, cell adhesion and phosphorylation of MAPKp42/44 and AKT. In summary, our results indicate that sex hormones are involved in the pathogenesis and progression of RMS, and therefore, their therapeutic application should be avoided in patients that have been diagnosed with RMS.

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“…Insulinlike growth factor-1 is known to activate porcine SC through the mTOR pathway . Treatment with IGF-1, FGF, EGF, or TGFβ increase proliferation rates in bovine satellite cells (Greene and Allen, 1991;Kamanga-Sollo et al, 2008Reiter et al, 2014). Furthermore, treatment of fused bovine SC cultures with IGF-1, FGF, EGF, or TGFβ results in increased protein synthesis rates (Kamanga-Sollo et al, 2004;Reiter et al, 2014).…”

Section: Effects Of Nutrition On Circulating Growth Factorsmentioning

confidence: 99%

“…Treatment with IGF-1, FGF, EGF, or TGFβ increase proliferation rates in bovine satellite cells (Greene and Allen, 1991;Kamanga-Sollo et al, 2008Reiter et al, 2014). Furthermore, treatment of fused bovine SC cultures with IGF-1, FGF, EGF, or TGFβ results in increased protein synthesis rates (Kamanga-Sollo et al, 2004;Reiter et al, 2014). However, treatment of bovine SC cultures with IGF-1 stimulates differentiation, whereas treatment with EGF, FGF, or TGFβ inhibits differentiation (Allen and Boxhorn, 1987;Greene and Allen, 1991).…”

Section: Effects Of Nutrition On Circulating Growth Factorsmentioning

confidence: 99%

TRIENNIAL GROWTH SYMPOSIUM: THE NUTRITION OF MUSCLE GROWTH: Impacts of nutrition on the proliferation and differentiation of satellite cells in livestock species1,2

Thornton

2019

Journal of Animal Science

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Epidermal growth factor receptor is required for estradiol-stimulated bovine satellite cell proliferation

Cited by 7 publications

References 38 publications

Role of G protein-coupled estrogen receptor-1 in estradiol 17β-induced alterations in protein synthesis and protein degradation rates in fused bovine satellite cell cultures

Role of G protein-coupled estrogen receptor-1 in estradiol 17β-induced alterations in protein synthesis and protein degradation rates in fused bovine satellite cell cultures

Human rhabdomyosarcoma cells express functional pituitary and gonadal sex hormone receptors: Therapeutic implications

TRIENNIAL GROWTH SYMPOSIUM: THE NUTRITION OF MUSCLE GROWTH: Impacts of nutrition on the proliferation and differentiation of satellite cells in livestock species1,2

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