Epidermal Water-Barrier Formation After Stripping of Normal Skin*

Spruit, D.; Malten, K. E.

doi:10.1038/jid.1965.83

Cited by 42 publications

(19 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Possible systematic errors during the regeneration of the skin are therefore repeated each time and do not influence the results of the curves. The relative accuracy of the measurement of water vapour loss does not exceed the physiological changes of the permeability of the skin [5].…”

Section: Methods Of Measuring the Water Vapour Lossmentioning

confidence: 99%

“…The m easurements taken on successive days yield a curve, representing the " regeneration of the w ater vapour loss" . The slope of the semi-logarithmically decreas ing curve, indicated by the " half regeneration tim e" t;/2, represents the regeneration rate of the damaged skin [5]. By stripping the horny layer is removed completely.…”

Section: Methodsmentioning

confidence: 99%

“…Three days after the injury, however, the water vapour loss of the skin has been restored to such an extent that the difference between the water vapour loss of the damaged and the normal skin has become very small. The measure ment of the regeneration rate in the second phase therefore calls for m ethods of greater accuracy [5], For the principles of such measure m ent we refer to an earlier issue of this journal [1]. This m ethod has been further developed.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Regeneration Rate of the Water Vapour Loss of Heavily Damaged Skin

Spruit¹,

Malten²

1966

Dermatology

Self Cite

View full text Add to dashboard Cite

Section: Methods Of Measuring the Water Vapour Lossmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Regeneration Rate of the Water Vapour Loss of Heavily Damaged Skin

Spruit¹,

Malten²

1966

Dermatology

Self Cite

View full text Add to dashboard Cite

“…S. aureus from the patients whose sebum lipids were collected was sampled by the ‘cup-scrub’ method [11] in the area adjacent to that used for collecting sebum on the upper arm of each subject. The suspension containing bacteria from the skin of AD patients was spread out on egg yolk-mannitol salt agar (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) and strains were classified as S. aureus based upon a reaction to lecithinase on the agar.…”

Section: Methodsmentioning

confidence: 99%

Deficient Production of Hexadecenoic Acid in the Skin Is Associated in Part with the Vulnerability of Atopic Dermatitis Patients to Colonization by <i>Staphylococcus aureus</i>

Takigawa

Nakagawa²,

Kuzukawa³

et al. 2005

Dermatology

117

107

View full text Add to dashboard Cite

Background and Objectives: As one of the major skin fatty acids, cis-6-hexadecenoic acid (C16:1Δ6) exhibits a specific antibacterial activity and might play a specific role in the defense mechanism against Staphylococcus aureus, in healthy subjects whereas S. aureus frequently colonizes the skin of patients with atopic dermatitis (AD). Methods: Fatty acid composition of sebum at the recovery level was analyzed by gas chromatography and S. aureus colonizing the skin was assessed by the ‘cup-scrub’ method (9 patients and 10 healthy controls). To evaluate in vivo effect of C16:1Δ6 on colonization, C16:1Δ6 was applied for 2 weeks on the upper arm skin of another group of AD patients (11 patients). Results: Analysis of sebum lipids revealed that there is a significant lower free C16:1Δ6 content in nonlesional skin from AD patients compared with healthy controls. This lower content is also accompanied by a significantly lower level of C16:1Δ6 in the total fatty acid composition of sebum (analyzed following hydrolysis). The lower level of free C16:1Δ6 correlated significantly (R² = 0.41, p < 0.01) with the numbers of S. aureus colonizing nonlesional skin. Topical application of free C16:1Δ6 on the skin of AD patients for 2 weeks abolished the markedly increased bacterial count in 6 out of the 8 AD patients tested. Conclusions: Free C16:1Δ6 may be involved in the defense mechanism against S. aureus in healthy skin and this deficit triggers the susceptibility of the skin to colonization by S. aureus in AD.

show abstract

“…It may be achieved by determining the coefficient of variation of the measurements. The variation coefficient of normal measurements of the water vapour loss is high [7]. In Table I it am ounts to 7.1 % at site A and 7.5 % at site B.…”

Section: Resultsmentioning

confidence: 87%