Epididymal Secretion and Resorption of Proteins

Cooper, Trevor G.

doi:10.1007/978-3-642-71471-9_15

Cited by 5 publications

(2 citation statements)

References 260 publications

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“…Also, in a similar study, Moteiti et al have shown a decrease in the percentage of HSPA2 positive spermatozoa in infertile men by flow cytometry ( 16 ). Normally, residual cytoplasm is removed from spermatozoa during spermiation and failure in the release of excess residual cytoplasm (ERC) could cause cytoplasm retention ( 27 ). HSPA2 is involved in cytoplasmic extrusion during sperm maturation, and a decrease in the level of HSPA2 leads to cytoplasmic retention and abnormal spermatozoa morphology ( 15 , 28 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia

Sabeti

Amidi

Kalantar

et al. 2017

IJRM

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Background:Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology.Objective:To compare the level of intracellular anion superoxide (O2–), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men.Materials and Methods:In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4%_ and total motility lower than 40% ) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O2– and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test.Results:The rate of CMA3+ spermatozoa in the case group was higher than controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were significantly lower than controls (p=0.001). Also, intracellular O2– levels in the case group were significantly higher than controls (p=0.001) and had positive correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm (r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81, p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between intracellular O2– and HSPA2 in the case group (r=0.041, p=0.79).Conclusion:We suggest that the increase in intracellular O2–, decrease in spermatozoa HSPA2+, and high percentages of spermatozoa with immature chromatin might be considered as etiologies of infertility in TA patients.

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Section: Discussionmentioning

confidence: 99%

Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia

Sabeti

Amidi

Kalantar

et al. 2017

IJRM

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“…The body of epididymis is attached with the lateral surface of testicle [10]. Whereas, head and body are involved in sperm maturation and tail is place for sperm storage [11]. Various studies [12,13] have exposed that spermatogenesis in rams is sensitive to increments in protein ingestion.…”

Section: Introductionmentioning

confidence: 99%

Puberty associated micro anatomical modification in testes and epididymis of Teddy goat (Capra hircus)

Khan¹

2019

PAB

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Despite some studies being conducted over past few decades, puberty associated histological changes in testes and epididymis of caprine is still largely unknown. The present study was conducted to evaluate the microanatomy of the testicles and epididymis through light microscope. Histometric parameters of testes and epididymis were measured through software Image J. Majority of seminiferous tubules (ST) become rounded in shape, increased parenchymal cells and space between them was increased in pubertal stage. In ST, peritubular cells, Sertoli cells and developing undifferentiated germinal cells were present at pubertal stage. In pre-pubertal stage the epididymal duct was lined by non-ciliated epithelium and become ciliated in pubertal stage. Luminal diameter of the tubules increased from pre-pubertal to pubertal stage in the tail region. The abundant loose connective tissue was observed in prepubertal as compared with pubertal stage. The values of histometerical parameters; diameter of seminiferous tubules, thickness of tunica albugenia, diameter of caput of epididymis tubule, corpus of epididymis tubule and cauda of epididymal tubules were significantly higher in pubertal stage than pre-pubertal stage of Teddy goat. Based on above findings it is concluded that puberty and age significantly affects micro anatomical aspects of reproductive organs of Teddy goats.

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Clusterin (SGP‐2) in epididymal luminal fluid and its association with epididymal spermatozoa in androgen‐deprived rats

Mattmueller

Hinton

1992

Molecular Reproduction Devel

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Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absence of androgen. We have previously demonstrated by two-dimensional (2-D) Western blot probing for clusterin that in epididymal fluid the amounts of clusterin were: caput greater than corpus greater than cauda. Luminal fluid from cauda epididymides was collected from control and orchiectomized rats (6 and 12 days) and orchiectomized animals that received testosterone implants. Equal volumes of fluid were analyzed by 2-D Western blot probing for clusterin. Following orchiectomy, there was an increase in clusterin in the luminal fluid after 6 days and maximal amount after 12 days compared with control cauda fluid. Orchiectomized animals which received testosterone treatment showed levels of clusterin comparable to that of controls. Serum clusterin was detected in fluid of orchiectomized animals with and without testosterone. Western blots of cauda sperm membrane extracts of control animals and orchiectomized animals treated with testosterone had a very low level of epididymal clusterin, whereas extracts collected from orchiectomized animals revealed high levels of clusterin. We suggest that, in the normal animal, clusterin is secreted into the lumen of the proximal epididymis where it binds to the sperm membrane. In the distal epididymis, clusterin dissociates from sperm and is processed (proteolysis/endocytosis). We hypothesize that, in the absence of androgen, the processing and regulation of clusterin is disrupted.

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Epididymal Secretion and Resorption of Proteins

Cited by 5 publications

References 260 publications

Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia

Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia

Puberty associated micro anatomical modification in testes and epididymis of Teddy goat (Capra hircus)

Clusterin (SGP‐2) in epididymal luminal fluid and its association with epididymal spermatozoa in androgen‐deprived rats

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