Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia

Sabeti, Parvin; Amidi, Fardin; Kalantar, S M; Gilani, Mohammad Ali Sedighi; Pourmasumi, Soheila; Najafi, Ali; Talebi, Ali Reza

doi:10.29252/ijrm.15.5.279

Cited by 13 publications

(9 citation statements)

References 35 publications

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“…We also found that in the two cohorts studied (N = 375 and N = 100), HDS was weakly correlated with CMA3. CMA3 directly competes with protamines in the sperm nucleus to bind GC-rich DNA domains, giving an indirect indication of protamine deficiency [50]. In addition, we show that HDS does not correlate at all with toluidine blue (TB) staining although some authors have reported the opposite [51].…”

Section: Discussionmentioning

confidence: 62%

Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?

Mohammadi

Tavalaee

Gharagozloo

et al. 2020

Basic Clin. Androl.

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Background: The Sperm Chromatin Structure Assay (SCSA®), in addition to identifying the DNA Fragmentation Index (DFI) also identifies High DNA satiability (HDS), supposed to reflect the nuclear compaction of spermatozoa. However, data on what exactly this parameter reveals, its relevance and usefulness are contradictory. In order to shed light on this situation, spermatozoa of a cohort (N = 397) of infertile men were subjected to the SCSA®, TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling) and CMA3 (Chromomycin A3) tests. In a smaller subcohort (N = 100), aniline blue (AB) and toluidine blue (TB) staining were performed in addition. The objective of this study was thus to answer the question of whether HDS is a relevant and reliable parameter to be taken into account? Results: HDS does not appear to be a reliable indicator of nuclear immaturity because it shows a weak correlation with the CMA3, AB and TB stains. The low correlation of HDS with sperm DNA fragmentation (TUNEL and SCSA®) and DNA condensation (CMA3, AB and TB) tests suggests that these two parameters could be decoupled. Unlike DFI and TUNEL, HDS has not been shown to correlate with classic clinical situations of male infertility (asthenozoospermia, teratozoospermia or astheno-teratozoospermia). Conclusion: HDS correlates poorly with most tests that focus specifically on the level of maturity of the sperm nucleus. To our knowledge, this study is the first to compare SCSA®, TUNEL, AB, TB and CMA3 assays on identical samples. It shows the potency, consistency and limitations of each test and the care that must be taken in their interpretation.

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Section: Discussionmentioning

confidence: 62%

Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?

Mohammadi

Tavalaee

Gharagozloo

et al. 2020

Basic Clin. Androl.

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“…According to SCSA data, both patient groups showed a significantly higher degree of DNA strand breaks in the sperm compared with the fertile individuals. Because increased reactive oxygen species and protamine deficiency are reported in UI and AT individuals (Mayorga‐Torres, Camargo, Cadavid, du Plessis, & Cardona Maya, ; Sabeti et al, ), we suggest that these factors may be responsible for DNA damages in our patients. In addition to DNA integrity, hyaluronic acid‐binding ability as a biomarker of cellular maturity could also influence the fertilizing potential of sperm.…”

Section: Discussionmentioning

confidence: 70%

“…Asthenoteratozoospermia is another common cause of male infertility in which, the failure of fertilization post‐ICSI could happen in a number of AT patients (Huang et al, ; Mangoli, Dandekar, Desai, & Mangoli, ). Although factors involved in the etiology of infertile men with asthenoteratozoospermia, such as genetic defects, DNA fragmentation, protamine deficiency, and oxidative stress, have been studied in the past (Avidan et al, ; Huang et al, ; Sabeti et al, ), the role of decreased expression and abnormal distribution of PLCζ, as a potential reason of male infertility, has been overlooked.…”

Section: Introductionmentioning

confidence: 99%

The alteration of PLCζ protein expression in unexplained infertile and asthenoteratozoospermic patients: A potential effect on sperm fertilization ability

Rahimizadeh

Topraggaleh

Nasr‐Esfahani

et al. 2019

Molecular Reproduction Devel

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Failed oocyte activation has been observed in unexplained infertile (UI) and asthenoteratozoospermic (AT) men. The deficiency of phospholipase C-zeta (PLCζ) could be a possible reason for such failures and has not been studied yet. We investigated the expression and localization of PLCζ protein in the sperms of patients with UI and AT conditions. The relationships between PLCζ-related parameters with male age, sperm characteristics, DNA integrity, and cellular maturity were assessed.Semen samples were collected from fertile (n = 40), UI (n = 40), and AT (n = 40) men.Subsequently, semen analysis, DNA fragmentation, hyaluronic acid-binding ability, and PLCζ level along with its distribution were evaluated using computer-assisted sperm analyzer, sperm chromatin structure assay (SCSA), hyaluronic acid-binding assay (HBA), western blot analysis and immunofluorescence microscopy, respectively.Unlike SCSA, the values of HBA, and PLCζ expression were significantly reduced in UI and AT patients compared to fertile men, whereas no significant differences were observed among the experimental groups in terms of PLCζ localization patterns. The regression analysis also showed that HBA is the only variable associated with PLCζ levels. Furthermore, the correlation of male age with PLCζ localization in postacrosomal, equatorial, and acrosomal+postacrosomal+equatorial (A+PA+E) patterns, as well as the relation of normal morphology, with the (A+PA+E) pattern, remained in the regression model. Our findings indicated that reduced PLCζ level along with the increased DNA fragmentation and impaired maturation may be possible etiologies of decreased fertilization in the studied subjects. K E Y W O R D Sasthenoteratozoospermia, oocyte activation, PLCζ, unexplained male infertility

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“…Levels of O 2 ˉ generation were measured using dihydroethidium florescent dye 42,43 . With the exception of the third day, the superoxide ion production was incremented in both types of PCs during storage.…”

Section: Resultsmentioning

confidence: 99%

The effect of gamma irradiation on platelet redox state during storage

2020

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Background As a method with insignificant adverse effects on in vitro quality of platelet concentrates (PCs), gamma irradiation is applied to abrogate the risk of transfusion‐associated graft‐vs‐host disease in vulnerable recipients. However, there is some evidence of lower posttransfusion responses and proteomic alterations in gamma‐irradiated platelets (PLTs), which raises some questions about their quality, safety, and efficacy. Since reactive oxygen species (ROS) are considered as markers of PLT storage lesion (PSL), the study presented here investigated oxidant state in gamma‐irradiated PCs. Study Design and Methods PLT‐rich plasma PC was split into two bags, one kept as control while other was subjected to gamma irradiation. Within 7 days of storage, the levels of intra‐PLT superoxide, H2O2, mitochondrial ROS, P‐selectin expression, and phosphatidylserine (PS) exposure were detected by flow cytometry while intracellular reduced glutathione (GSH), glucose concentration, and lactate dehydrogenase (LDH) activity were measured by enzymocolorimetric method. Results GSH decreased, while ROS generation and LDH activity increased, during storage. Gamma irradiation significantly attenuated GSH whereas increased ROS generation in earlier and later stages of storage associated with either P‐selectin or PS exposure increments. Conclusion Gamma irradiation can significantly increase cytosolic ROS generation in two distinct phases, one upon irradiation and another later in longer‐stored PCs. While earlier ROS influx seems to be governed by direct effect of irradiation, the second phase of oxidant stress is presumably due to the storage‐dependent PLT activation. Intriguingly, these observations were also in line with early P‐selectin increments and increased PS exposure in longer‐stored PLTs. Given the mutual link between ROS generation and PLT activation, further investigation is required to explore the effect of gamma irradiation on the induction of PSL.

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Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia

Cited by 13 publications

References 35 publications

Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?

Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?

The alteration of PLCζ protein expression in unexplained infertile and asthenoteratozoospermic patients: A potential effect on sperm fertilization ability

The effect of gamma irradiation on platelet redox state during storage

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