(—)-Epigallocatechin gallate (EGCG) prevents isoprenaline-induced cardiac toxicity by stabilizing cardiac marker enzymes and membrane-bound ATPases

Abstract

Intake of tea flavonoids has been reported to reduce the incidence of cardiovascular disease. The present study was undertaken to investigate the preventive effect of (-)epigallocatechin gallate (EGCG) on heart weight, cardiac marker enzymes, membrane-bound ATPases and electrolytes in isoprenaline (ISO)-induced myocardial infarcted (MI) Wistar rats. Rats subcutaneously administered ISO 100 mgkg(-1) at intervals of 24 h for 2 days resulted in significant increases in heart weight and the activities of cardiac marker enzymes such as creatine kinase, creatine kinase-MB, lactate dehydrogenase (LDH), aspartate transaminase and alanine transaminase in serum, and significant decreases in the activities of these enzymes in the myocardium. ISO injection also increased levels of LDH isoenzymes (LDH 1 and LDH 2). The activity of Na+/K+ ATPase was decreased significantly and the activities of Ca2+ and Mg2+ ATPases were increased significantly in ISO-induced MI rats. Furthermore, the levels of potassium were lowered and the levels of sodium and calcium were increased in ISO-induced MI rats. Prior treatment with EGCG (10, 20 and 30 mgkg(-1)) daily for a period of 21 days reduced the effects of ISO on heart weight, activities of cardiac marker enzymes and membrane bound-ATPases and levels of LDH 1 and LDH 2 and electrolytes. Thus, EGCG exhibits beneficial effects on these enzymes and electrolytes. The observed effects may be due to the antioxidant and membrane-stabilizing effects of EGCG in ISO-induced MI rats.

Tomato lycopene attenuates myocardial infarction induced by isoproterenol: Electrocardiographic, biochemical and anti–apoptotic study

“…A significant increase in serum uric acid and decrease in total protein and heart vitamin E levels was observed in ISO injected rats, which is in line with the previous reports [18,21] . Large cohort studies have shown that uric acid is an important independent risk factor for cardiovascular mortality and in the development of MI.…”

Pomegranate (Punica granatum L.) Juice Supplementation Attenuates Isoproterenol-Induced Cardiac Necrosis in Rats

“…channel protein is known to cause increased influx of Ca 2? into myocardium following IP treatment [34]. In our study, pre-supplementation with PJ could resist to a greater extent the perturbations in Na ?…”

“…Lipid peroxidation, a type of oxidative deterioration of polyunsaturated fatty acids has been linked with altered membrane structure, and inactivation of SOD and CAT resulting in accumulation of superoxide anion, which further damages the myocardium [34]. In this context, the observed depletion in GSH and Vit C and decreased activity levels of SOD and CAT can be correlated with significant increment in cardiac LPO in IP-treated rats and indicate higher susceptibility of myocardium to oxidative damage.…”

Protective Effect of Betaine on Changes in the Levels of Membrane-bound ATPase activity and Mineral Status in Experimentally Induced Myocardial Infarction in Wistar Rats

“…Cellular injury is associated with alterations in mineral homeostasis. Thus, these enzymes and ions play a vital role in the pathology of myocardial infarction [16]. Hence, we undertook the present study with new cohorts of animals to evaluate the protective effect of betaine on these membrane-bound ATPases, lipid peroxides, myocardial sulfhydryl levels, and mineral status in isoprenaline-induced myocardial infarction in rats.…”