Pomegranate (Punica granatum L.) Juice Supplementation Attenuates Isoproterenol-Induced Cardiac Necrosis in Rats

Abstract

The aim of the present study was to evaluate the efficacy of pre-supplementation with pomegranate (Punica granatum L.) juice (PJ) on heart weight, infarct size, plasma marker enzymes of cardiac damage, lipid peroxidation, endogenous enzymatic and non-enzymatic antioxidants, cardiac ATPases and histopathology of isoproterenol (IP)-induced cardiac necrosis (CN) in rats. Rats treated with IP (85 mg/kg, s.c.) for 2 days at an interval of 24 h caused significant (P < 0.05) infarct in myocardium and increase in heart weight, lipid peroxidation (LPO), activity levels of Ca(+2) ATPase and plasma marker enzymes, while there was significant (P < 0.05) decrease in endogenous enzymatic and non-enzymatic antioxidants and Na(+)-K(+) and Mg(+2)ATPases. Pre-supplementation with PJ for 30 consecutive days and treated with IP on days 29th and 30th showed significantly (P < 0.05) lesser increase in heart weight, infarct size, plasma marker enzymes, lipid peroxidation, Ca(+2) ATPase and a significant protective effect in endogenous enzymatic and non-enzymatic antioxidants, Na(+)-K(+) and Mg(+2) ATPases compared to IP alone treated group. Present study provides first scientific report on protective effect of supplementation of Pomegranate juice against IP-induced CN in rats.

A Combination of Melatonin and Alpha Lipoic Acid has Greater Cardioprotective Effect than Either of them Singly Against Cadmium-Induced Oxidative Damage

“…Level of MDA, a major oxidation product of peroxidized poly unsaturated fatty acids, has been considered to be an important indicator of lipid peroxidation [42]. In the current study, high levels of cardiac MDA in cadmiumexposed rats indicate heightened lipid peroxidation and prevalent oxidative stress as has been reported for cadmium and other heavy metal intoxication [43,44].…”

Dietary chitosan supplementation attenuates isoprenaline-induced oxidative stress in rat myocardium

“…There was a significant (p < 0.05) increase in the level of lipid peroxidation in the heart tissue of Group III isoprenaline-administered rats as compared to controls (Table 3). This concurs with the findings of Jadeja et al [24], which indicated that the higher vulnerability of myocardium to peroxidative damage was mainly due to deterioration in the level of free radical scavengers. Lipid peroxidation of membranes is delimited by the availability of substrate in the form of PUFA, the availability of inducers such as free radicals and excited state molecules to promote propagation, the antioxidant defense status of environment and the physical status of the membrane lipid bilayer [9].…”

Pomegranate phytoconstituents blunt the inflammatory cascade in a chemically induced rodent model of hepatocellular carcinogenesis

“…An absence of cardiotoxicity following long-term PE consumption as opposed to various synthetic inhibitors of COX-2, evidenced from our noninvasive echocardiographic assessment, represents an important finding of our current study. Several laboratories have reported the protective effects of pomegranate juice or extract against experimentally induced cardiotoxicity in rats [52,53].…”