Epigallocatechin gallate, ellagic acid, and rosmarinic acid perturb dNTP pools and inhibit de novo DNA synthesis and proliferation of human HL-60 promyelocytic leukemia cells: Synergism with arabinofuranosylcytosine

Saiko, Philipp; Steinmann, Marie-Thérèse; Schuster, Heike; Graser, Geraldine; Bressler, Sabine; Giessrigl, Benedikt; Lackner, Andreas; Grusch, Michael; Krupitza, Georg; Bagó-Horváth, Zsuzsanna; Jaeger, Walter; Fritzer‐Szekeres, Monika; Szekeres, Thomas

doi:10.1016/j.phymed.2014.11.017

Cited by 33 publications

(21 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rosmarinic acid (40 μM) increased macrophage differentiation induced by ATRA which was mediated by an increase in CD11b expression on the cell surface [103]. In HL-60 leukemia cells, RA (50–150 μM) inhibited cell growth and induced apoptosis, which was associated with decreased dNTP levels [104]. CCRF-CEM, CEM/ADR5000 leukemia cells treated with RA (3–100 μM) developed increased cytotoxicity, apoptosis, necrosis, cell cycle arrest and caspase-independent apoptosis which was mediated by increased PARP cleavage and blockage of p65 nuclear translocation [105].…”

Section: Anticancer Effects Of Rosmarinic Acid (Ra): In Vitro Studiesmentioning

confidence: 99%

Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols

2016

View full text Add to dashboard Cite

Cancer cells display enhanced growth rates and a resistance to apoptosis. The ability of cancer cells to evade homeostasis and proliferate uncontrollably while avoiding programmed cell death/apoptosis is acquired through mutations to key signaling molecules, which regulate pathways involved in cell proliferation and survival. Compounds of plant origin, including food components, have attracted scientific attention for use as agents for cancer prevention and treatment. The exploration into natural products offers great opportunity to evaluate new anticancer agents as well as understand novel and potentially relevant mechanisms of action. Rosemary extract has been reported to have antioxidant, anti-inflammatory, antidiabetic and anticancer properties. Rosemary extract contains many polyphenols with carnosic acid and rosmarinic acid found in highest concentrations. The present review summarizes the existing in vitro and in vivo studies focusing on the anticancer effects of rosemary extract and the rosemary extract polyphenols carnosic acid and rosmarinic acid, and their effects on key signaling molecules.

show abstract

Section: Anticancer Effects Of Rosmarinic Acid (Ra): In Vitro Studiesmentioning

confidence: 99%

Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols

2016

View full text Add to dashboard Cite

show abstract

“…Once cell proliferation or inappropriate cell death occurs, it typically results in a tumor, and it has been demonstrated that the regulation of the cell cycle is an important mechanism in tumorigenesis. Arrest of the various phases of the cell cycle may be used to inhibit cell proliferation and induce differentiation or apoptosis (30,31). Cancer is a disease that causes disorders in the cell cycle and uncontrolled proliferation due to the combined effects of hereditary and environmental factors (32).…”

Section: Discussionmentioning

confidence: 99%

pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC-4 gastric tumor cells

et al. 2017

View full text Add to dashboard Cite

The aim of the present study was to investigate the effect of purified Omphalia lapidescens protein (pPeOp) extracted by polyvinylpyrrolidone from the fungus Omphalia lapidescens Schroet on the proliferation and cell cycle progression of MC-4 human gastric tumor cells. Using polyvinylpyrrolidone, pPeOp was extracted from O. lapidescens Schroet. MC-4 cells were cultured with 30, 60 or 90 µg/ml pPeOp, with 5-fluorouracil used as a positive control. Survival rates of treated cells were significantly decreased compared with those of the untreated control group in a dose-dependent manner. Using flow cytometric analysis, cells treated with pPeOp were demonstrated to arrest in S phase and exhibit abnormal G0/G1 and G2/M phase cell cycle distribution. In addition, a wound healing assay demonstrated that pPeOp significantly inhibited the migration of MC-4 cells. The mRNA and protein expression levels of cyclin D1/cyclin-dependent kinase (CDK) 4, cyclin B/CDK1, cyclin A/CDK2, matrix metalloproteinase (MMP)-2 and MMP-9 were determined using reverse transcription-quantitative polymerase chain reaction analysis and western blotting. The mRNA expression level of CDK4 and cyclin A was significantly increased compared with the untreated control; however, cyclin D1, CDK1, CDK2, cyclin B, MMP-2, and MMP-9 exhibited a significantly decreased mRNA expression level, indicating that there is a negative association between concentration and cyclin D1 expression levels. The expression of the cycle arrest-associated proteins and migration-associated proteins examined were similar to the observed mRNA expression levels. In conclusion, pPeOp was identified to inhibit migration of and cause S phase cell cycle arrest in MC-4 cells.

show abstract

“…These molecules improve chemotherapy as sensitizers including the flavonol EGCG present in green tea, which inhibits the chymotrypsin-like activity of proteasome b5 subunit [101][102][103][104]. Other flavonoids also inhibit the proteasome including genistein [105], luteolin [106], apigenin [107], chrysin [106], quercetin [108], the non-flavonoid polyphenol curcumin [109] and the hydrolyzable tannins [110].…”

Section: Protein Degradation Pathwaysmentioning

confidence: 99%

Targeting the unfolded protein response for disease intervention

Rivas

Vidal

Hetz

2015

Expert Opinion on Therapeutic Targets

View full text Add to dashboard Cite

Therapeutic strategies directed to regulate the activity of different UPR signaling arms may reduce stress levels with a therapeutic gain. Recent drug discovery efforts have identified small molecules that target specific UPR components, providing protection on various disease models. However, important side effects are predicted in the chronic administration due to the fundamental role of the UPR in highly secretory organs such as liver and pancreas. To overcome these problems, we propose the use of combinatorial treatments of selected drugs with natural compounds that are known to modulate the ER proteostasis network.

show abstract

Epigallocatechin gallate, ellagic acid, and rosmarinic acid perturb dNTP pools and inhibit de novo DNA synthesis and proliferation of human HL-60 promyelocytic leukemia cells: Synergism with arabinofuranosylcytosine

Cited by 33 publications

References 42 publications

Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols

Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols

pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC-4 gastric tumor cells

Targeting the unfolded protein response for disease intervention

Contact Info

Product

Resources

About