pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC-4 gastric tumor cells

Abstract

The aim of the present study was to investigate the effect of purified Omphalia lapidescens protein (pPeOp) extracted by polyvinylpyrrolidone from the fungus Omphalia lapidescens Schroet on the proliferation and cell cycle progression of MC-4 human gastric tumor cells. Using polyvinylpyrrolidone, pPeOp was extracted from O. lapidescens Schroet. MC-4 cells were cultured with 30, 60 or 90 µg/ml pPeOp, with 5-fluorouracil used as a positive control. Survival rates of treated cells were significantly decreased compared with those of the untreated control group in a dose-dependent manner. Using flow cytometric analysis, cells treated with pPeOp were demonstrated to arrest in S phase and exhibit abnormal G0/G1 and G2/M phase cell cycle distribution. In addition, a wound healing assay demonstrated that pPeOp significantly inhibited the migration of MC-4 cells. The mRNA and protein expression levels of cyclin D1/cyclin-dependent kinase (CDK) 4, cyclin B/CDK1, cyclin A/CDK2, matrix metalloproteinase (MMP)-2 and MMP-9 were determined using reverse transcription-quantitative polymerase chain reaction analysis and western blotting. The mRNA expression level of CDK4 and cyclin A was significantly increased compared with the untreated control; however, cyclin D1, CDK1, CDK2, cyclin B, MMP-2, and MMP-9 exhibited a significantly decreased mRNA expression level, indicating that there is a negative association between concentration and cyclin D1 expression levels. The expression of the cycle arrest-associated proteins and migration-associated proteins examined were similar to the observed mRNA expression levels. In conclusion, pPeOp was identified to inhibit migration of and cause S phase cell cycle arrest in MC-4 cells.

Anti-Gastric Cancer Effect of Purified Omphalia Lapidescens Protein via Regulating the JAK/STAT3 Signaling Pathway

“…The human normal gastric cell line MC-1 and human gastric cancer cell lines MC-4, which had been reported at previous study [8], were provided from Zhejiang Provincial Center for Disease and Prevention Centre (Zhejiang CDC). Human normal gastric cell line MC-1 and human gastric cancer cells were cultured with RPMI-1640 medium with 5% (v/v) fetal bovine serum (Gibco®), 100 U/ml penicillin and 100 U/ml streptomycin in a humidified 5% CO2 atmosphere at 37°C.…”

“…pPeOp is a protein extracted from O.lapidescens with PVP extraction buffer [7]. Previous studies demonstrated that pPeOp suppresses metastasis, arrests cell cycle and induces apoptosis in MC-4 gastric cancer cells without causing toxicity to MC-1 normal gastric cells [8]. Thus, the underlying anti-tumor mechanism of pPeOp deserves further research.…”

pPe <italic>O</italic>p inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway

“…Due to the urgent clinical need, researchers are working to develop various antitumour drugs targeting gastric cancer. Omphalia lapidescens , a traditional Chinese medicine, has been reported to have a significant inhibitory effect on malignant tumors [1] . We previously identified a bioactive protein (pPe O p) from O .…”