Epimerization of disaccharides by enzyme preparations from Ruminococcus albus

Tyler, Talmage; Leatherwood, J. M.

doi:10.1016/0003-9861(67)90466-3

Cited by 57 publications

(31 citation statements)

References 6 publications

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“…Tyler and Leatherwood (1967) found the first known cellobiose 2-epimerase (CE; EC 5.1.3.11), which catalyzes the reversible epimerization between cellobiose and 4-O-β-D-glucopyranosyl-D-mannose (Glc-Man), in the culture broth of R.…”

Section: Introductionmentioning

confidence: 99%

Site-directed mutagenesis of possible catalytic residues of cellobiose 2-epimerase from Ruminococcus albus

et al. 2009

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sp. CH1 and on the computer-aided model building of the tertiary structure of RaCE, we performed site-directed mutagenesis of possible catalytic residues in the enzyme, and the mutants were expressed in Escherichia coli cells. It was found that R52, H243, E246, W249, M251, W304, E308, H374, and M378 were absolutely required for the activity of RaCE. F114 and W303 (and possibly R377) also contributed to catalysis. These possible catalytic residues protruded into or near the active-site cleft surrounded by the inner -helices in the predicted (/) 6 core barrel structure of RaCE.

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Section: Introductionmentioning

confidence: 99%

Site-directed mutagenesis of possible catalytic residues of cellobiose 2-epimerase from Ruminococcus albus

et al. 2009

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“…Tyler & Leatherwood (1967) found for the first time that the ruminal R. albus 7 (ATCC 27210 T ) possessed an epimerization activity that catalyzed hydroxyl stereoisomerism at the C‐2 position of the glucose moiety of cellobiose and generated 4‐ O ‐β‐ d ‐glucopyranosyl‐ d ‐mannose (Glc‐Man). However, in the last four decades, there has been no report on the purification, enzymatic properties, and nucleotide sequence of the cellobiose 2‐epimerase (CE; EC 5.1.3.11).…”

Section: Introductionmentioning

confidence: 99%

Cloning and sequencing of the gene for cellobiose 2-epimerase from a ruminal strain ofEubacterium cellulosolvens

Taguchi

Senoura

Hamada

et al. 2008

FEMS Microbiology Letters

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Cellobiose 2-epimerase (CE; EC 5.1.3.11) is known to catalyze the reversible epimerization of cellobiose to 4-O-beta-D-glucopyranosyl-D-mannose in Ruminococcus albus cells. Here, we report a CE in a ruminal strain of Eubacterium cellulosolvens for the first time. The nucleotide sequence of the CE had an ORF of 1218 bp (405 amino acids; 46 963.3 Da). The CE from E. cellulosolvens showed 44-54% identity to N-acyl-D-glucosamine 2-epimerase-like hypothetical proteins in the genomes of Coprococcus eutactus, Faecalibacterium prausnitzii, Clostridium phytofermentans, Caldicellulosiruptor saccharolyticus, and Eubacterium siraeum. Surprisingly, it exhibited only 46% identity to a CE from R. albus. The recombinant enzyme expressed in Escherichia coli was purified by two-step chromatography. The purified enzyme had a molecular mass of 46.7 kDa and exhibited optimal activity at around 35 degrees C and pH 7.0-8.5. In addition to cello-oligosaccharides, it converted lactose to epilactose (4-O-beta-D-galactopyranosyl-D-mannose).

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“…1) It catalyzes the reversible epimerization of cellobiose to 4-O--D-glucopyranosyl-D-mannose (Glc-Man). Recently, we succeeded in the purification of a CE protein (RaCE; formerly named CE-NE1) from R. albus NE1 cells and in the identification of the encoding gene.…”

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confidence: 99%

Identification of the Cellobiose 2-Epimerase Gene in the Genome ofBacteroides fragilisNCTC 9343

Senoura

Taguchi

Ito

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Epimerization of disaccharides by enzyme preparations from Ruminococcus albus

Cited by 57 publications

References 6 publications

Site-directed mutagenesis of possible catalytic residues of cellobiose 2-epimerase from Ruminococcus albus

Site-directed mutagenesis of possible catalytic residues of cellobiose 2-epimerase from Ruminococcus albus

Cloning and sequencing of the gene for cellobiose 2-epimerase from a ruminal strain ofEubacterium cellulosolvens

Identification of the Cellobiose 2-Epimerase Gene in the Genome ofBacteroides fragilisNCTC 9343

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