EPR and FTIR Spectroscopies Study of Human Blood after Irradiation

Abstract

In the present work we report results of investigations of human blood before and after radioisotopeTc99mdiagnosis by electron paramagnetic resonance (EPR) and Fourier transform infrared (FTIR) spectroscopies. It is shown that EPR can detect the concentration of methaemoglobin and transferrin ions more accurately than any other technique. FTIR spectra indicated that radiation caused conformational and concentration changes of proteins. Hierarchical cluster analysis (HCA) was created as time-saving tool for discrimination of initial and irradiatedin vivohuman blood samples.

Microfluidics and Raman microscopy: current applications and future challenges

“…[journal], [year], [vol], 00-00 | 15 226 Bacteria on milk 227 Bacteria in food 228 Counterfeit drugs 229 Human blood 230 Principal component analysis (PCA)…”

“…243 Regarding Raman-microfluidics, this method is also able to distinguish peaks that are generated from microfluidic system materials, such as PDMS, glass, water or other solvents and even excess contaminants. 225 Respiratory syncytial virus strain 226 Bacteria on milk 227 Bacteria in food 228 Counterfeit drugs 229 Human blood 230 Principal component analysis (PCA) Gestational hypertension 233 Identification of medicine counterfeits 234 Laryngeal cancer diagnosis 236 Saliva from lung cancer patents 235 Deconvolution Breakdown of complex Raman spectra into known bond peaks in order to identify and quantify its components YES NO Human skin samples 242 Cancer cells 243 Carbon nanotubes 244 Wavenumber correlation…”

“…FTIR can be used for characterisation of complex and, in particular, biological samples in microfluidics. 230 Alternatively, attenuated total reflection infrared (ATR-IR) spectroscopic imaging is suitable for samples where transmission FTIR is impractical. ATR-IR uses a high refractive index crystal that allows IR radiation to reflect within the ATR crystal several times.…”

Prediction of type 1 diabetes with machine learning algorithms based on FTIR spectral data in peripheral blood mononuclear cells

“…In last decades FTIR has been used as an effective and nondestructive method for monitoring cellular alterations. 37 FTIR spectrum provides ngerprints of biomolecules that can be correlated with metabolic changes 10,38,39 FTIR spectral data analyses have been used for quantication of proteins, nucleic acids, lipids, carbohydrates 40,41 evaluation of the cell metabolic responses to environmental factors, 42,43 diagnostics of different types of cancer and distinguishing cancer and normal cells at high sensitivity, specicity and accuracy, 44,45 and metabolic diseases such as diabetes. [46][47][48][49] FTIR is a non-destructive, labelfree, chemically specic, high-throughput, easy, fast, relatively cheap, and accessible method for detection of uctuations of biomolecules in bio-samples of patients.…”