EPR Spin Probe Study of New Micellar Systems

Wasserman, A. M.; Motyakin, M. V.; Yasina, L. L.; Zakharova, Yulia A.; Matveenko, V. N.; Shulevich, Yu. V.; Rogovina, L.Z.

doi:10.1007/s00723-009-0106-3

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…All measurements were performed at 295 K. The microwave power and the modulation amplitude were kept constant for all measurements at 3 mW and 0.3 mT, respectively. It was reported that the SDS micelle structure was not perturbed considerably if lower proportions of 5-DSA were used 9,30 and hence its concentration was fixed at a suitable value i.e., 0.5 mM, in all EPR measurements. The preparation of the solutions containing the spin label (5-DSA) was made in the same way as that found in the report.…”

Section: Instruments and Methodsmentioning

confidence: 99%

Physicochemical perspectives (aggregation, structure and dynamics) of interaction between pluronic (L31) and surfactant (SDS)

Prameela

Kumar

Pan

et al. 2015

Phys. Chem. Chem. Phys.

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The influence of the water soluble non-ionic tri-block copolymer PEO-PPO-PEO [poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide)] i.e., E2P16E2 (L31) on the microstructure and self-aggregation dynamics of the anionic surfactant sodium dodecylsulfate (SDS) in aqueous solution was investigated using cloud point (CP), isothermal titration calorimetry (ITC), high resolution nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR), and small-angle neutron scattering (SANS) measurements. CP provided the thermodynamic information on the Gibbs free energy, enthalpy, entropy and heat capacity changes pertaining to the phase separation of the system at elevated temperature. The ITC and NMR self-diffusion measurements helped to understand the nature of the binding isotherms of SDS in the presence of L31 in terms of the formation of mixed aggregates and free SDS micelles in solution. EPR analysis provided the micro-viscosity of the spin probe 5-DSA in terms of rotational correlation time. The SANS study indicated the presence of prolate ellipsoidal mixed aggregates, whose size increased with the increasing addition of L31. At a large [L31], SANS also revealed the progressive decreasing size of the ellipsoidal mixed aggregates of SDS-L31 into nearly globular forms with the increasing SDS addition. Wrapping of the spherical SDS micelles by L31 was also corroborated from (13)C NMR and SANS measurements.

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Section: Instruments and Methodsmentioning

confidence: 99%

Physicochemical perspectives (aggregation, structure and dynamics) of interaction between pluronic (L31) and surfactant (SDS)

Prameela

Kumar

Pan

et al. 2015

Phys. Chem. Chem. Phys.

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“…They chose to follow the splitting and shape of the ESR signal from nitroxide spin labels coadsorbed with the surfactant on the alumina surface to identify clustering, aggregation, and other phenomena. A second ESR study on micellar systems concluded that the local mobility of cationic long-chain detergents vary little as the length of a chain is increased, but that the order parameter increases noticeably and a phase change takes place from solid to a micellar state . 5-Doxylstearic acid and 16-doxylstearic acid, which have low solubility in water and tend to be localized in the hydrophobic parts of complex systems, were used there as spin probes; those molecules build themselves into the micellar system so that their paramagnetic fragment is located in the hydrocarbon core of the micelle at a fixed distance from the interface and their motion is correlated with that of the surrounding surfactant molecules.…”

Section: Other Spectroscopiesmentioning

confidence: 99%

Probing Liquid/Solid Interfaces at the Molecular Level

2012

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“…Molecular dynamics studies report that the chain dihedral and CH 2 order parameters in dodecylphosphocholine micelles were comparable to that of the bilayer (24) and that the dihedral transition rate was slower in micelles than that of a DPPC bilayer (24,25). A comparison of electron paramagnetic resonance (EPR) studies of bilayers (26)(27)(28)(29) and micelles (30)(31)(32)(33)(34)(35) suggests that the rotational dynamics of the alkyl chains can be similar or different depending on the detergent micelle and bilayer. Micelle dynamics could be modulated by numerous intermolecular forces between the detergent monomers.…”

Section: Introductionmentioning

confidence: 99%

The Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS

Kieber

Ono

Oliver

et al. 2019

Biophysical Journal

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The dynamics of phosphocholine and maltoside micelles, detergents frequently used for membrane protein structure determination, were investigated using electron paramagnetic resonance of spin probes doped into the micelles. Specifically, phosphocholines are frequently used detergents in NMR studies, and maltosides are frequently used in x-ray crystallography structure determination. Beyond the structural and electrostatic differences, this study aimed to determine whether there are differences in the local chain dynamics (i.e., fluidity). The nitroxide probe rotational dynamics in longer chain detergents is more restricted than in shorter chain detergents, and maltoside micelles are more restricted than phosphocholine micelles. Furthermore, the micelle microviscosity can be modulated with mixtures, as demonstrated with mixtures of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate with n-dodecylphosphocholine, n-tetradecylphosphocholine, n-decyl-b-D-maltoside, or n-dodecyl-b-D-maltoside. These results indicate that observed differences in membrane protein stability in these detergents could be due to fluidity in addition to the already determined structural differences.

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EPR Spin Probe Study of New Micellar Systems

Cited by 9 publications

References 19 publications

Physicochemical perspectives (aggregation, structure and dynamics) of interaction between pluronic (L31) and surfactant (SDS)

Physicochemical perspectives (aggregation, structure and dynamics) of interaction between pluronic (L31) and surfactant (SDS)

Probing Liquid/Solid Interfaces at the Molecular Level

The Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS

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