EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide, Inhibits Liver Cancer Cell Growth and Adhesion

Abstract

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.

Antioxidant Effects of Irisin in Liver Diseases: Mechanistic Insights

“…And it is discovered that Moringa oleifera fruit could cause ROS-mediated apoptosis in human liver cancer HepG2 cells [44]. What is more, Wang et al demonstrated that EPS364, a deep-sea bacterial exopolysaccharide, exhibited an antitumor trait by inducing hepatic cell death by upregulating ROS production, supporting the idea that oxidative stress could trigger cell death [45]. And the idea is likewise proofed by another research where diTFPP/C2-ceramide increased hepatic cell death by increasing ROS levels [39].…”

Marine Polysaccharides for Wound Dressings Application: An Overview

“…Gelling agent Metal-complexion Antioxidant [220] Vibrio alginolyticus 364 deep-sea Anti-tumour [223] Rhodothermus marinus DSM 4252 T Shallow marine hot springs Antioxidant Anti-haemolytic Anti-thrombotic [224] Winogradsky sp. CAL384 and Shewanella sp.…”

Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I

“…Extraction of total RNA and synthesis of cDNA were performed as mentioned previously (45). The full-length cDNA sequence of β1-integrin was obtained by PCR.…”