Equine articular cartilage chondrocytes: opening the black box

Hall, Andrew C.; Bush, Peter G.; Davidson, Margaret E; Kempson, S. A.

doi:10.2746/042516403775600442

Cited by 7 publications

(6 citation statements)

References 15 publications

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“…Lactated Ringer's solution (273 mOsm/L) is the standard-of-care irrigation solution used for equine arthroscopic surgery. [9][10][11] Normal saline (0.9% NaCl solution and 300 mOsm/L) has been shown to inhibit synthesis of GAG by chondrocytes and has therefore fallen out of favor in recent years. 21 The proteoglycan content of the extracellular matrix dictates the osmolality of the extracellular environment to which cartilage is exposed.…”

Section: Discussionmentioning

confidence: 99%

The use of a hyperosmolar irrigation solution is safe in an equine stifle joint model but does not reduce joint swelling

Hayden

Stoker

Johnson

et al. 2022

ajvr

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OBJECTIVE To determine the following: (1) whether an irrigation solution that is hyperosmolar (HYPER) relative to synovial fluid decreases tissue extravasation during an arthroscopic protocol when compared to a relatively hypoosmolar solution, (2) the safety of a HYPER solution based on viability of joint tissues following joint irrigation, and (3) if the use of a HYPER solution decreases water content in stifle joint tissue. ANIMALS 8 adult horses. PROCEDURES A prospective, blinded, randomized controlled trial was performed to compare lactated Ringer’s solution (LRS; 273 mOsm/L) and a HYPER (600 mOsm/L) irrigation solution for routine medial femorotibial joint (MFTJ) arthroscopy. Primary outcomes included quantification of periarticular fluid retention based on measured changes in defined stifle joint girth and ultrasonographic (US) criteria. Water content of tissue samples was assessed. The viability of articular cartilage was determined using a microscopic fluorescent cell viability staining system. RESULTS No significant difference in postprocedural joint swelling was observed between LRS and HYPER treatment groups. Percent increments in femorotibial joint dimensions (mean ± SD) were seen in both treatment groups based on US (LRS, 83.9 ± 84.6%; HYPER, 131.2 ± 144.9%) and caliper measurements (LRS 5.5 ± 4.3%; HYPER 7.5 ± 5.8%) (P ≤ .05). Chondrocyte viability and tissue water content were maintained in both treatment groups, and differences were not statistically significant. CLINICAL RELEVANCE Doubling the osmolarity of an irrigation solution used routinely for arthroscopy does not result in detrimental effects on chondrocyte viability or tissue water content. However, use of a relatively HYPER irrigation solution did not attenuate procedural tissue swelling of the equine stifle joint.

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Section: Discussionmentioning

confidence: 99%

The use of a hyperosmolar irrigation solution is safe in an equine stifle joint model but does not reduce joint swelling

Hayden

Stoker

Johnson

et al. 2022

ajvr

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“…These cell groups provide the synthesis and degradation of the ECM compnents, to support growth and regeneration, through the maintenance the gene expression and metabolism responded by mechanical stimuli. Extracellular matrix and proteoglycans are expressed by chondrocytes in articular joints [60][61][62][63][64].…”

Section: Healthy Cartilage Tissuementioning

confidence: 99%

ADAMTS Proteases: Potential Biomarkers and Novel Therapeutic Targets for Cartilage Health

Kandir¹

2020

Equine Science

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The equine locomotor system's health plays a key role on athletic performance. Bone and joint diseases are the major causes of lameness. Poor performance and diseases lead to great economic loss to equestrian sports and horse breeders. Therefore, prevention, early diagnosis, and therapy of joint diseases are important. A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) proteinase family plays an important role in many physiological processes such as tissue reorganization, coagulation, and angiogenesis. Aggrecan proteinases ADAMTS-4 and ADAMTS-5 are physiologically responsible for the restructuring with enzymatic cleavage of the cartilage, specific biomarkers in the synovium or body fluids for early diagnosis, and potential specific therapeutic targets in order to their role on degenerative joint diseases physiopathology in humans and various animals.

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“…Diese nehmen ca. 5 bis 10 % des Gewebevolumens ein (Bruckner et al, 1994;Archer et al, 2003;Hall et al, 2003), sind reich an Zellorganellen und synthetisieren die Makromoleküle der sie umgebenden Matrix. In Ermangelung direkter Kontakte oder Verbindungen der Chondrozyten untereinander (Kuettner et al, 1982) erfolgt die interzelluläre Kommunikation ausschließlich über die Extrazellulärmatrix (Kuettner, 1992).…”

Section: 1) Hyaliner Knorpel -Anatomie Physiologie Und Funktionunclassified

Tissue Engineering von Knorpelersatzgewebe – Mechanische Stimulation in der In-vitro-Kultivierung von humanen Chondrozyten

et al. 2010

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Equine articular cartilage chondrocytes: opening the black box

Cited by 7 publications

References 15 publications

The use of a hyperosmolar irrigation solution is safe in an equine stifle joint model but does not reduce joint swelling

The use of a hyperosmolar irrigation solution is safe in an equine stifle joint model but does not reduce joint swelling

ADAMTS Proteases: Potential Biomarkers and Novel Therapeutic Targets for Cartilage Health

Tissue Engineering von Knorpelersatzgewebe – Mechanische Stimulation in der In-vitro-Kultivierung von humanen Chondrozyten

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