Horse trailers are a common form of transportation for horses and ponies and often require the animal to travel alone or with a single companion. The current study investigated the effect of transporting horses alone, in company or with an acrylic safety mirror (measuring 81 x 61.5 cm) that provided surrogate companionship. The behavioural and physiological responses of twelve mature horses during a 30-minute journey by trailer under the three treatments were compared.Behaviour (vocalisation, eating, head-tossing, pawing, and head turning) was recorded. In order to assess circulatory changes that occur as part of the response to transport, heart rate (HR), rectal (T r ) and ear-pinna (T p ) temperatures were recorded. When travelling with a live companion significantly less time was spent vocalising (p<0.001), head turning (p<0.001), head-tossing (p<0.01) and pawing (p<0.01); eating behaviour increased (p<0.05). Physiological responses (increases in HR and T r and decreases in T p ) were also significantly reduced when travelling with a live companion (p<0.01). Travelling with the mirror did not significantly affect physiological responses compared with travelling alone, but the rise in T r and fall in T p was reduced (p=0.052 and p=0.051 respectively) and can be considered a trend. When travelling with a mirror significantly less time was spent turning the head (p<0.01), vocalising (p<0.05) and head tossing (p<0.05); eating behaviour increased (p<0.05). The only significant difference between travelling with a live 1 companion and a mirror was that the time spent turning the head round was less with a live companion (p<0.05). The provision of surrogate companionship in the form of a mirror was found to be preferable to travelling alone, but where possible a live companion is recommended. Isolation during transportation was found to suppress feeding behaviour. Although peripheral blood flow (T p ) has been used to assess transport stress in other species it has not previously been used in the horse. Further evaluation of this non-invasive measure is now required.