Eradication of potato virus Y and S from potato by chemotherapy of cultured axillary bud tips

Wambugu, Florence; Secor, Gary A.; Gudmestad, Neil C.

doi:10.1007/bf02853476

Cited by 31 publications

(11 citation statements)

References 9 publications

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“…These percentages were decreased to 50% in Burren and 56% in Binella when ribavirin's concentration (10 mg l −1 ) was added with the meristem 300 µm in length (Table 3). Similar results was reported by (Gudmestad et al 1985) when he added the ribavirin (20 mg l −1 ) to the meristem's medium. ese results did not agree with that recorded by (Gianni 2002) when he added the Ribavirin (50 mg l −1 ) to achieve the highest percentage of free-virus plants.…”

supporting

confidence: 84%

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Al-Maarri

Massa

Al-Biski

2012

Plant Biotechnology

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supporting

confidence: 84%

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Al-Maarri

Massa

Al-Biski

2012

Plant Biotechnology

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“…Ironically, PLRV is one of the easiest viruses to eliminate using meristem-tip culture (20). The lack of PVY eradication was rather surprising, particularly as previous studies with ribavirin reported plantlets testing free from PVY (3,4,33). The differences may be due to the fact that in the previous studies, shoot tips from greenhouse-grown plants, meristems, or protoplasts were cultured rather than nodal cuttings from plantlets and, hence, a smaller or less organized tissue segment with a reduced virus reservoir was exposed to ribavirin.…”

Section: Discussionmentioning

confidence: 93%

“…Adding chemicals with antiviral properties to plant tissue culture medium and excising shoot tips has, in the case of the synthetic riboside ribavirin (1-P-D-ribofuranosyl-1,2,4-triazole-3-carboxamide, trade name virazole (29)), resulted eliminating three of the major potato viruses, PVX and potato viruses S (PVS) and Y (PVY) (11,33). Using a combination of heat and ribavirin treatments on in vitro nodal cuttings, we were able to eliminate PVS from the potato x DCsirCe…”

Section: Introductionmentioning

confidence: 99%

Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets

Griffiths¹,

Slack²,

Dodds³

1990

Can. J. Bot.

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Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets. Can. J. Bot. 68: 1515-1521.Potato (Solanurn tuberosum) plantlets were established in vitro to evaluate the effectiveness of chemicals and heat therapy for antiviral activity. Ribavirin was shown to be the most active chemical. Further studies were performed in which plantlets established from nodal cuttings were exposed to an alternating 4-h cycle of 35-31°C and ribavirin (20 mg/L) therapy for 4 weeks, and then tested quantitatively for virus titer by the enzyme-linked immunosorbent assay. Plantlets were further propagated via nodal cuttings at room temperature without exposure to ribavirin. Virus assays were performed and virus-free plantlets were grown out as mature plants for a final virus assay. Ribavirin alone or in combination with heat therapy was effective in reducing potato virus M, S, and X titers (between 10-and 60-fold). Virus-free plants were obtained from both treatments. For potato viruses Y and leafroll, about a fourfold reduction in titer was observed after the ribavirin-heat treatments. When multiple viruses were present, quantitative (10-to 20-fold) reductions in potato viruses M, S, X, and leafroll were observed and resulted in plants free of potato viruses M, S, and X. This protocol enables the investigator to quantitatively monitor the impact of a single variable on the virus-host interaction. We have alsoTeen able to demonstrate that virus elimination can be obtained without a requisite meristem-tip excision step. GRIFFITHS, H. M., SLACK, S. A . , et DODDS, J. H. 1990. Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets. Can. J. Bot. 68 : 1515-1521. Des plantules de Pomme de terre (Solanurn tuberosurn) furent Ctablies in vitro afin d'Cvaluer l'efficacitt de certains produits chimiques et de la thermothtrapie sur 1'activitC antivirale. La ribavirine a Ct C la substance chimique la plus active. D'autres Ctudes furent rCalisCes oh des plantules provenant de sections nodales furent exposCes i une alternance de cycles thkrapeutiques de 35-31°C d'une durCe de 4 h en presence de ribavirine (20 mg/L), pendant 4 semaines, et puis testtes quantitativement pour la concentration en virus par l'essai immunosorbant d'enzymes liCes. Les plantules furent de nouveau propagees via des sections nodales i temperature de la pikce et en l'absence de ribavirine. Des essais viraux furent rCalists et des plantules sans virus furent cultivtes jusqu'au stade de plantes matures en vue d'un essai viral final. La ribavirine seule ou en combinaison avec la themotherapie a rCduit la concentration des virus de la pomme de terre M, S et X de 10 i 60 fois. Des plantes sans virus furent obtenues des deux traitements. Quant au virus Y et i celui de I'enroulement, l'on a observC une rkduction quadruple de la concentration pour les traitements ribavirine-chaleur. Lorsque plusieurs virus Ctaient prksents, des reductions quantitatives des virus M, S, X et de l'enroulement (rtductions de 10 i 20 fois) furen...

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“…Cassells and Long (1980) reported production of virus-free plants from cucumber mosaic virus and potato virus X infecting tobacco explants by inclusion of a synthetic broad-spectrum antiviral compound Ribavirin (Virazole) in the culture medium. In a subsequent study, Wambugu et al (1985) reported that Ribavirin was phytotoxic to potato axillary bud tips at all concentrations tested, but exhibited considerable inhibitory effect on virus replication (72%) even at the lowest concentration (5 mg/l). In a subsequent study, Wambugu et al (1985) reported that Ribavirin was phytotoxic to potato axillary bud tips at all concentrations tested, but exhibited considerable inhibitory effect on virus replication (72%) even at the lowest concentration (5 mg/l).…”

Section: Chemotherapy and Virus Eliminationmentioning

confidence: 92%

Meristem and Shoot Tip Culture: Requirements and Applications

Nehra¹,

Kartha²

1994

Plant Cell and Tissue Culture

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Eradication of potato virus Y and S from potato by chemotherapy of cultured axillary bud tips

Cited by 31 publications

References 9 publications

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets

Meristem and Shoot Tip Culture: Requirements and Applications

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