Eriosema laurentii De Wild (Leguminosae) methanol extract has estrogenic properties and prevents menopausal symptoms in ovariectomized Wistar rats

Ateba, Sylvin Benjamin; Njamen, Dieudonné; Medjakovic, Svjetlana; Hobiger, Stefanie; Mbanya, Jean Claude; Jungbauer, Alois; Krenn, Liselotte

doi:10.1016/j.jep.2013.08.050

Cited by 26 publications

(28 citation statements)

References 64 publications

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“…Postmenopausal changes in lipid profiles may contribute to an increased risk of cardiovascular disease [2]. As reported by previous studies, estrogen administration decreased serum TC and LDL-C levels but increased TG levels in OVX rats [24]. TGs are considered to play a key role in cardiovascular risk in postmenopausal women [25]; therefore, increases in TG levels are considered undesirable changes associated with estrogen therapy.…”

Section: Discussionmentioning

confidence: 87%

Black Cohosh (<i>Actaea racemosa</i> L.) Improves Serum Lipid Profiles and Vasomotor Responses in Ovariectomized Rats

Kim¹,

Song²,

Kim³

et al. 2017

JFNR

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We investigated the effects of long-term administration of black cohosh extract (BcEx) on serum lipid profiles and vasomotor responses in ovariectomized (OVX) rats and compared them with those of rats administered 17β-estradiol (E2) or raloxifene, a selective estrogen receptor modulator. Vehicle (OVX-or sham-control), BcEx (0.5 or 3.0 mg/kg/day), E2 (0.5 mg/kg/day), or raloxifene (2.5 mg/kg/day) were injected subcutaneously for 5 weeks, and serum lipid profiles and vasomotor responses were measured at the end of the treatment. BcEx lowered total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels, but did not affect high-density lipoprotein cholesterol or triglyceride (TG) levels. Raloxifene showed a similar effect to that of BcEx, while E2 attenuated the increase in TC and LDL-C levels and significantly increased TG levels. The vascular relaxation induced by carbachol increased significantly in norepinephrine-precontracted aortic rings isolated from BcEx-, E2-, or raloxifene-treated rats. No change in uterine weight was observed in the BcEx-treated group. The raloxifene-treated group showed a similar trend as that of the BcEx-treated group, but E2 significantly increased uterine weight. These results suggest that long-term administration of BcEx behaves similar to the selective estrogen receptor modulator raloxifene.

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Section: Discussionmentioning

confidence: 87%

Black Cohosh (<i>Actaea racemosa</i> L.) Improves Serum Lipid Profiles and Vasomotor Responses in Ovariectomized Rats

Kim¹,

Song²,

Kim³

et al. 2017

JFNR

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“…As a result, OVX-induced changes, including noticeable increases in food consumption, body weight and gains, and abdominal fat depositions with adipocyte hypertrophy, were significantly inhibited by treatment with estradiol and 1, 2, and 4 ml/kg PCS. Estrogen depletion in an OVX animal model was observed along with significant increases in food intake and changes in body fat depositions, especially in the abdominal cavity (54)(55)(56). In addition, obesity-induced OVX mice exhibited an accumulation of fat deposition and cellular hypertrophy through the expansion of intra-abdominal adipose tissue (57,58).…”

Section: Discussionmentioning

confidence: 99%

“…As estrogens are shown to act on numerous female target organs, such as the uterus, vagina, and skeletal and cardiovascular systems (88,89), menopausal women may experience climacterium symptoms due to lack of estrogen (90,91). Loss of estrogen is accompanied by atrophy of the uterus and vagina (56,91). Phytoestrogenic effects of isoflavonoids cause increases in uterine masses via uterine water imbibitions and/or a cell proliferation (88,92), which are mediated through ERα (93)(94)(95).…”

Section: Discussionmentioning

confidence: 99%

Anti-climacterium effects of pomegranate concentrated solutions in ovariectomized ddY mice

Kang¹,

Choi²,

Kim³

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. In the present study, the complex anti-climacterium potential of standardized pomegranate concentrated solution (PCS) was investigated using bilateral ovariectomy (OVX) female ddY mice. Changes in body weight and gain during experimental periods, food consumption, serum estradiol levels, total body and abdominal fat densities, abdominal fat pads, and uterus weights were observed, along with the histopathology of abdominal fat pads and uterus for anti-obesity and estrogenic effects. In addition, liver weights, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, and histopathological inspections were performed to explore the hepato-protective effects. Serum total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein, and triglyceride (TG) levels were monitored for hypolipidemic effects with total body and femur mean bone mineral density (BMD), right femur wet, dry and ash weights, strength, serum osteocalcin, bone-specific alkaline phosphatase (bALP) contents, and histological and histomorphometrical analyses for anti-osteoporosis activity. As a result of OVX, notable increases in body weight and gains, food consumption, abdominal fat mass densities, weights of abdominal fat pads deposited in the abdominal cavity, and serum AST, ALT, TC, LDL, TG, and osteocalcin levels were observed, along with decreases in the uterus, liver, and femur weights, mean total body and femur BMD, femur strength, serum bALP, and estradiol levels. In addition, marked hypertrophic alterations in adipocytes located in the deposited abdominal fat pads, liver steatosis, uterine disused atrophic changes, and decreases in bone mass and structures of the femur were also observed in OVX control mice with significant increases in bone resorption markers based on histopathological and histomorphometrical analysis. However, these estrogen-deficient climacterium symptoms were significantly (P<0.05 or P<0.01) inhibited after 84 days of continuous treatment with estradiol and PCS (1, 2 and 4 ml/kg), respectively. The present results suggested that PCS was able to effectively inhibit or refine the climacterium symptoms, including obesity, hyperlipidemia, hepatic steatosis, and osteoporosis, induced by OVX in ddY mice.

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“…HPLC-UV analysis was performed on a Shimadzu Prominence HPLC system with a Luna 5μm C18 (2) 100Å (250 × 4.60 mm I.D.) column by a HPLC method previously described [11]. LC-MS was conducted on an UltiMate 3000RSLC-series system (Dionex, Germering, Germany) coupled to an HCT 3D quadrupole ion trap mass spectrometer equipped with an orthogonal ESI source (Bruker Daltonics, Bremen, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…In Cameroon, E. laurentii preparations are used for the treatment of infertility and various gynecological and menopausal complaints. Our previous study showed that the methanol extract of the aerial parts of E. laurentii exhibited agonistic activities at the estrogen receptor α and the aryl hydrocarbon receptor in yeast transactivation assays and prevented menopause-related symptoms induced by ovariectomy in rats [11]. In addition, the safety profile of this extract indicated a broad safety margin following acute and subchronic oral administration [12].…”

Section: Introductionmentioning

confidence: 99%

Lupinalbin A as the most potent estrogen receptor α- and aryl hydrocarbon receptor agonist in Eriosema laurentii de Wild. (Leguminosae)

Ateba

Njamen

Medjakovic

et al. 2014

BMC Complement Altern Med

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BackgroundEriosema laurentii De Wild. (Leguminosae) is a plant used in Cameroon against infertility and gynecological or menopausal complaints. In our previous report, a methanol extract of its aerial parts was shown to exhibit estrogenic and aryl hydrocarbon receptor agonistic activities in vitro and to prevent menopausal symptoms in ovariectomized Wistar rats.MethodsIn order to determine the major estrogen receptor α (ERα) agonists in the extract, an activity-guided fractionation was performed using the ERα yeast screen. To check whether the ERα active fractions/compounds also accounted for the aryl hydrocarbon receptor (AhR) agonistic activity of the crude methanol extract, they were further tested on the AhR yeast screen.ResultsThis study led to the identification of 2′-hydroxygenistein, lupinalbin A and genistein as major estrogenic principles of the extract. 2′-hydroxygenistein and lupinalbin A were, for the first time, also shown to possess an AhR agonistic activity, whereas genistein was not active in this assay. In addition, it was possible to deduce structure-activity relationships.ConclusionsThese results suggest that the identified compounds are the major active principles responsible for the estrogenic and AhR agonistic activities of the crude methanol extract of the aerial parts of Eriosema laurentii.

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Eriosema laurentii De Wild (Leguminosae) methanol extract has estrogenic properties and prevents menopausal symptoms in ovariectomized Wistar rats

Cited by 26 publications

References 64 publications

Black Cohosh (<i>Actaea racemosa</i> L.) Improves Serum Lipid Profiles and Vasomotor Responses in Ovariectomized Rats

Black Cohosh (<i>Actaea racemosa</i> L.) Improves Serum Lipid Profiles and Vasomotor Responses in Ovariectomized Rats

Anti-climacterium effects of pomegranate concentrated solutions in ovariectomized ddY mice

Lupinalbin A as the most potent estrogen receptor α- and aryl hydrocarbon receptor agonist in Eriosema laurentii de Wild. (Leguminosae)

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