Esculetin enhances TRAIL-induced apoptosis through DR5 upregulation in human oral cancer SAS cells

Kok, Sang‐Heng; Yeh, Cheng-Chang; Chen, Mei‐Ling; Kuo, Mark Yen‐Ping

doi:10.1016/j.oraloncology.2009.07.018

Cited by 51 publications

(51 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown the inhibitory effect of esculetin on cell proliferation in many cell lines (31)(32)(33)(34)37). The present study also shows that esculetin inhibits the growth of colon cancer HCT116 cells in a time-and dosedependent manner, as is evident by cell viability and DNA synthesis.…”

Section: Discussionsupporting

confidence: 84%

See 1 more Smart Citation

Esculetin inhibits cell proliferation through the Ras/ERK1/2 pathway in human colon cancer cells

Park

Park²,

Lim³

et al. 2010

Oncol Rep

View full text Add to dashboard Cite

Abstract. Esculetin, a phenolic compound, has been shown to inhibit the growth of colon tumors in animal studies. However, the roles of signaling pathways and cell cycle regulation in the esculetin-induced inhibition of cancer cell growth, remain to be elucidated. The present study suggests a novel mechanism for the Ras/ERK1/2 pathway in esculetintreated human colon cancer HCT116 cells. The treatment of cells with esculetin resulted in significant growth inhibition and G1 phase cell cycle arrest, which led to the downregulation of cyclin and cyclin-dependent kinase (CDK) expressions. This G1 phase cell cycle arrest was associated with the up-regulation of p27KIP expression. In addition, ERK1/2 was activated by esculetin. The pre-treatment of cells with the MEK1/2-specific inhibitor, PD98059, blocked the p27KIP expression induced by esculetin. Blockage of the ERK1/2 function consistently prevented the inhibition of cell proliferation and decreased G1 phase cell cycle protein levels. Furthermore, Ras activation was increased by the esculetin treatment. Transient transfection of the dominant negative Ras (RasN17) mutant gene abolished both the ERK1/2 activity and p27KIP expression induced by esculetin. Finally, the overexpression of RasN17 suppressed the esculetininduced reduction in cell proliferation and cell cycle proteins. In conclusion, these results indicate that the Ras/ERK1/2 pathway is mediated by the p27KIP1 induction, leading to a reduction in cyclin/CDK complexes in the esculetin-induced inhibition of colon cancer cell growth. Overall, these findings indicate that the molecular action of esculetin has therapeutic potential for the treatment of colon malignancies.

show abstract

Section: Discussionsupporting

confidence: 84%

“…It is known to inhibit cancer cell proliferation in both in vitro and in vivo models (34,37). However, little is known about the activation and the possible role of the Ras/ERK1/2 signaling pathway that underlies cell cycle regulation in the esculetin-induced inhibition of cancer cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Esculetin inhibits cell proliferation through the Ras/ERK1/2 pathway in human colon cancer cells

Park

Park²,

Lim³

et al. 2010

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…These compounds can trigger apoptosis through either the intrinsic mitochondrial pathway or the death receptor extrinsic pathway depending on the cancer cell type (Chu et al 2001;Mantena et al 2006;Meeran & Katiyar 2007;Kok et al 2009;Ji et al 2009;You et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Pereira

Bester

Soundy

et al. 2016

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Pelargonium sidoides DC (Geraniaceae) is an important medicinal plant indigenous to South Africa and Lesotho. Previous studies have shown that root extracts are rich in polyphenolic compounds with antibacterial, antiviral and immunomodulatory activities. Little is known regarding the anticancer properties of Pelargonium sidoides extracts. Objective This study evaluates the anti-proliferative effects of a Pelargonium sidoides radix mother tincture (PST). Materials and methods The PST was characterized by LC-MS/MS. Anti-proliferative activity was evaluated in the pre-screen panel of the National Cancer Institute (NCI-H460, MCF-7 and SF-268) and the Jurkat leukaemia cell line at concentrations of 0-150 mg/mL. The effect on cell growth was determined with sulphorhodamine B and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays after 72 h. The effect on cell cycle and apoptosis induction in Jurkat cells was determined by flow cytometry with propidium iodide and Annexin V: fluorescein isothiocyanate staining. Results Dihydroxycoumarin sulphates, gallic acid as well as gallocatechin dimers and trimers were characterized in PST by mass spectrometry. Moderate anti-proliferative effects with GI 50 values between 40 and 80 mg/mL were observed in the NCI-pre-screen panel. Strong activity observed with Jurkat cells with a GI 50 value of 6.2 mg/mL, significantly better than positive control 5-fluorouracil (GI 50 value of 9.7 mg/mL). The PST arrested Jurkat cells at the G 0 /G 1 phase of the cell cycle and increased the apoptotic cells from 9% to 21%, while the dead cells increased from 4% to 17%. Conclusion We present evidence that P. sidoides has cancer cell type-specific anti-proliferative effects and may be a source of novel anticancer molecules. ARTICLE HISTORY

show abstract

“…Se-Jung lee 1,3 , ung-Soo lee 1 , Wun-Jae KiM 2 and Sung-KWon Moon anti-oxidant activity, suppression of xanthine oxidase activity, platelet aggregation, inhibition of human leukemia cell growth and anti-tumor activity (18)(19)(20)(21)(22). in addition, esculetin is known to inhibit VSMc prolifertion in vitro and neointima formation in vivo (23,24).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of esculetin on migration, invasion and matrix metalloproteinase-9 expression in TNF-α-induced vascular smooth muscle cells

Lee

Kim

et al. 2011

Mol Med Rep

View full text Add to dashboard Cite

Abstract. esculetin, a potent non-competitive inhibitor of lipoxygenase, has been shown to inhibit vascular smooth muscle cell (VSMc) proliferation. However, the effect of esculetin on the matrix metalloproteinase-9 (MMP-9) regulation responsible for cell migration and invasion has not been previously investigated. The results of the present study showed the esculetin (12.5-25 µg/ml) induced the inhibition of migration and invasion in tumor necrosis factor-α (TnF-α)-treated VSMc, as demonstrated by a matrigel invasion assay and wound healing analysis. However, esculetin did not affect cell viability in TnF-α-treated VSMc under 0-25 µg/ml concentration conditions. in addition, both zymographic and immunoblot experiments showed that esculetin suppressed the TnF-α-induced expression of MMP-9 in VSMc in a dose-dependent manner. Furthermore, the treatment of cells with esculetin decreased the activity of the TnF-α-induced MMP-9 promoter, which was driven by a luciferase reporter. Finally, esculetin reduced the binding activities of nuclear factor-κB (nF-κB) and activator protein-1 (aP-1), which are cis-elements present in the promoter of the MMP-9 gene, in TnF-α-treated VSMc. Taken together, these results demonstrated that esculetin decreased the migration and invasion of cells by suppressing MMP-9 expression, which subsequently reduced the binding activities of nF-κB and aP-1 in TnF-α-treated VSMC. These novel findings provide basic information for effective therapeutic treatment with esculetin for atherosclerotic disease.

show abstract

Esculetin enhances TRAIL-induced apoptosis through DR5 upregulation in human oral cancer SAS cells

Cited by 51 publications

References 37 publications

Esculetin inhibits cell proliferation through the Ras/ERK1/2 pathway in human colon cancer cells

Esculetin inhibits cell proliferation through the Ras/ERK1/2 pathway in human colon cancer cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Inhibitory effect of esculetin on migration, invasion and matrix metalloproteinase-9 expression in TNF-α-induced vascular smooth muscle cells

Contact Info

Product

Resources

About

Esculetin enhances TRAIL-induced apoptosis through DR5 upregulation in human oral cancer SAS cells

Cited by 51 publications

References 37 publications

Esculetin inhibits cell proliferation through the Ras/ERK1/2 pathway in human colon cancer cells

Esculetin inhibits cell proliferation through the Ras/ERK1/2 pathway in human colon cancer cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Inhibitory effect of esculetin on migration, invasion and matrix metalloproteinase-9 expression in TNF-α-induced vascular smooth muscle cells

Contact Info

Product

Resources

About

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells