Especies rickettsiales en casos humanos con síndrome febril agudo inespecífico en Perú

Palacios-Salvatierra, Rosa; Cáceres-Rey, Omar; Vasquez-Dominguez, Andres; Mosquera-Visaloth, Patricia; Anaya, Elizabeth

doi:10.17843/rpmesp.2018.354.3646

Cited by 19 publications

(14 citation statements)

References 20 publications

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“…Gene sequences identical or almost identical (> 99.9% identity) to those from our study were detected in cat fleas from California, Texas and Georgia within the U.S. and in cat fleas and other vectors in countries in Central and South America, Europe, Africa and in Asia ( Table 1). The R. asembonensis detected here is similar (> 99%) to genotypes obtained from human blood in Peru 19 and Malaysia 20 , from Long-tailed macaques (Macaca fascicularis) in Malaysia 21 and from cats in Thailand 22 .…”

Section: Rickettsia Detection In Cat Fleassupporting

confidence: 77%

“…Our unanticipated study uncovered two different mitochondrial lineages of human-biting cat fleas infected with Rickettsia asembonensis. This flea-borne spotted fever Rickettsia species falls within the transitional group 26 and has been associated with human pathogenicity in Peru 19 and in Malaysia 20 . Rickettsia asembonensis was detected in the blood of healthy cynomolgus monkeys (Macaca fascicularis) in Malaysia 21 and in blood samples from cats in Thailand 22 , showing that this bacterium infects wild and domestic mammals, which in turn can act as reservoirs for human infections.…”

Section: Discussionmentioning

confidence: 99%

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Metagenomic analysis of human-biting cat fleas in urban northeastern United States of America reveals an emerging zoonotic pathogen

Ferreira

Fonseca

Hamilton

et al. 2020

Sci Rep

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An infestation of cat fleas in a research center led to the detection of two genotypes of Ctenocephalides felis biting humans in New Jersey, USA. The rarer flea genotype had an 83% incidence of Rickettsia asembonensis, a recently described bacterium closely related to R. felis, a known human pathogen. A metagenomics analysis developed in under a week recovered the entire R. asembonensis genome at high coverage and matched it to identical or almost identical (> 99% similarity) strains reported worldwide. Our study exposes the potential of cat fleas as vectors of human pathogens in crowded northeastern U.S, cities and suburbs where free-ranging cats are abundant. Furthermore, it demonstrates the power of metagenomics to glean large amounts of comparative data regarding both emerging vectors and their pathogens.

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Section: Rickettsia Detection In Cat Fleassupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Metagenomic analysis of human-biting cat fleas in urban northeastern United States of America reveals an emerging zoonotic pathogen

Ferreira

Fonseca

Hamilton

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The organism was known originally only from fleas and other arthropods until 2016, when sequences similar to those obtained from the Thai-Myanmar border study were amplified from the blood of a febrile child in Malaysia [96]. Following this report, R. asembonensis DNA has been detected in samples from febrile patients in Peru [120] and in two human cases in Zambia [121], although no categorical link with symptomatic disease was possible in the latter study. Moreover, R. asembonensis-like DNA sequences have been obtained from the blood of non-human mammals, including macaques [93] and domestic ruminants [94] in Malaysia.…”

Section: Discussionmentioning

confidence: 93%

“…Following this report, R. asembonensis DNA has been detected in samples from febrile patients in Peru [120] and in two human cases in Zambia [121], although no categorical link with symptomatic disease was possible in the latter study. Moreover, R.…”

Section: Discussionmentioning

confidence: 99%

Metagenomics of culture isolates and insect tissue illuminate the evolution of Wolbachia, Rickettsia and Bartonella symbionts in Ctenocephalides spp. fleas

et al. 2023

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While fleas are often perceived simply as a biting nuisance and a cause of allergic dermatitis, they represent important disease vectors worldwide, especially for bacterial zoonoses such as plague (transmitted by rodent fleas) and some of the rickettsioses and bartonelloses. The cosmopolitan cat (Ctenocephalides felis) and dog (Ctenocephalides canis) fleas, as well as Ctenocephalides orientis (restricted to tropical and subtropical Asia), breed in human dwellings and are vectors of cat-scratch fever (caused by Bartonella spp.) and Rickettsia spp., including Rickettsia felis (agent of flea-borne spotted fever) and Rickettsia asembonensis , a suspected pathogen. These Rickettsia spp. are members of a phylogenetic clade known as the ‘transitional group’, which includes both human pathogens and arthropod-specific endosymbionts. The relatively depauperate flea microbiome can also contain other endosymbionts, including a diverse range of Wolbachia strains. Here, we present circularized genome assemblies for two C. orientis-derived pathogens ( Bartonella clarridgeiae and R. asembonensis ) from Malaysia, a novel Wolbachia strain (wCori), and the C. orientis mitochondrion; all were obtained by direct metagenomic sequencing of flea tissues. Moreover, we isolated two Wolbachia strains from Malaysian C. felis into tick cell culture and recovered circularized genome assemblies for both, one of which (wCfeF) is newly sequenced. We demonstrate that the three Wolbachia strains are representatives of different major clades (‘supergroups’), two of which appear to be flea-specific. These Wolbachia genomes exhibit unique combinations of features associated with reproductive parasitism or mutualism, including prophage WO, cytoplasmic incompatibility factors and the biotin operon of obligate intracellular microbes. The first circularized assembly for R. asembonensis includes a plasmid with a markedly different structure and gene content compared to the published plasmid; moreover, this novel plasmid was also detected in cat flea metagenomes from the USA. Analysis of loci under positive selection in the transitional group revealed genes involved in host–pathogen interactions that may facilitate host switching. Finally, the first B. clarridgeiae genome from Asia exhibited large-scale genome stability compared to isolates from other continents, except for SNPs in regions predicted to mediate interactions with the vertebrate host. These findings highlight the paucity of data on the genomic diversity of Ctenocephalides-associated bacteria and raise questions regarding how interactions between members of the flea microbiome might influence vector competence.

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“…The organism was known originally only from fleas and other arthropods until 2016, when sequences similar to those obtained from the Thai-Myanmar border study were amplified from the blood of a febrile child in Malaysia [110]. Following this report, R. asembonensis DNA has been detected in samples from febrile patients in Peru [111] and in two human cases in Zambia [112], although no categoric link with symptomatic disease was possible in the latter study. Moreover, R. asembonensis -like DNA sequences have been obtained from the blood of non-human mammals, including macaques [113] and domestic ruminants [114] in Malaysia.…”

Section: Discussionmentioning

confidence: 99%

Metagenomics of culture isolates and insect tissue illuminate the evolution ofWolbachia,RickettsiaandBartonellasymbionts inCtenocephalidesspp. fleas

Beliavskaia

Tan

Sinha

et al. 2023

Preprint

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While fleas are often perceived simply as a biting nuisance and cause of allergic dermatitis, they represent important disease vectors worldwide, especially for bacterial zoonoses such as plague (transmitted by rodent fleas) and some of the rickettsioses and bartonelloses. The cosmopolitan cat (Ctenocephalidesfelis) and dog (C. canis) fleas, as well asC. orientis(restricted to tropical and subtropical Asia), breed in human dwellings and are vectors of cat-scratch fever (caused byBartonellaspp.) andRickettsiaspp. of the so-called "transitional group". The latter includesR. felis(agent of flea-borne spotted fever) andR. asembonensis, an emerging pathogen. The relatively depauperate flea microbiome can also contain arthropod-specific endosymbionts, including a diverse range ofWolbachiastrains. Here, we present circularized genome assemblies for twoC. orientis-associated pathogens (Bartonella clarridgeiaeandR. asembonensis) from Malaysia, a novelWolbachiastrain (wCori), and theC. orientismitochondrion; all obtained by direct metagenomic sequencing of flea tissues. Moreover, we isolated twoWolbachiastrains from MalaysianC. felisinto tick cell culture and recovered circularized genome assemblies for both, one of which (wCfeF) is newly sequenced. We demonstrate that the threeWolbachiastrains are representatives of different major clades ("supergroups"), two of which appear to be flea-specific. TheseWolbachiagenomes exhibit unique combinations of features associated with reproductive parasitism or mutualism, including prophage WO, cytoplasmic incompatibility factors, and the biotin operon of obligate intracellular microbes. The first circularized assembly forR. asembonensisincludes a plasmid with a markedly different structure and gene content compared to the published plasmid; moreover, this novel plasmid was also detected in cat flea metagenomes from the US. Analysis of loci under positive selection in the transitional group revealed genes involved in host-pathogen interactions that may facilitate host switching. Finally, the firstB. clarridgeiaegenome from Asia exhibited largescale genome stability compared to isolates from other continents, except for SNPs in regions predicted to mediate interactions with the vertebrate host. These findings highlight the paucity of data on the genomic diversity ofCtenocephalides-associated bacteria and raise questions regarding how interactions between members of the flea microbiome might influence vector competence.

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Especies rickettsiales en casos humanos con síndrome febril agudo inespecífico en Perú

Cited by 19 publications

References 20 publications

Metagenomic analysis of human-biting cat fleas in urban northeastern United States of America reveals an emerging zoonotic pathogen

Metagenomic analysis of human-biting cat fleas in urban northeastern United States of America reveals an emerging zoonotic pathogen

Metagenomics of culture isolates and insect tissue illuminate the evolution of Wolbachia, Rickettsia and Bartonella symbionts in Ctenocephalides spp. fleas

Metagenomics of culture isolates and insect tissue illuminate the evolution ofWolbachia,RickettsiaandBartonellasymbionts inCtenocephalidesspp. fleas

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