Esplenectomia subtotal, em cães, com preservação do pólo inferior(PI) suprido por vasos do ligamento gastroesplênico

Paulo, Danilo Nagib Salomão; Silva, Alcino Lázaro da; Cintra, Luiz Cálice; Bof, Antônio Mauro; Santiago, Danielle Campos; Ribeiro, Gilberto Barroso

doi:10.1590/s0100-69911999000300005

Cited by 14 publications

(25 citation statements)

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“…The upper and medial spleen was devascularized and sectioned for removal of the upper 2/3 of the organ. The lower pole was kept irrigated by gastrosplenic ligament vessels according to Paulo et al 9,10 The length, width and thickness of the pole were measured in its central part in the two groups with a pachymeter. After removal of the lower pole, animals of group 1 were sacrificed by administration of a lethal dose of sodium pentobarbital.…”

Section: Methodsmentioning

confidence: 99%

“…5 Therefore, total or partial spleen preservation is indicated whenever possible. Our group has described subtotal splenectomy with preservation of the lower pole even after ligature of major spleen blood vessels in dogs 9 and rats. 10 Analysis of lower pole function based on lipid metabolism in dogs showed an early decline on postoperative day 7 which, however, improved on postoperative day 60.…”

Section: Introductionmentioning

confidence: 99%

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Growth of the lower spleen pole remaining after subtotal splenectomy in rats

et al. 2008

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PURPOSE: To determine whether the lower pole of the spleen grows after subtotal splenectomy following ligature of major spleen blood vessels. METHODS: Thirty-nine Wistar rats (328.8 ± 27.8 g) submitted to subtotal splenectomy with preservation of the lower splenic pole were divided into two groups: group 1 (control, n=20), immediate removal of the lower pole; group 2 (n=19), removal of the pole on postoperative day 80. The length, width and thickness of the pole were measured. In the control group, mean percent pole weight was calculated immediately after surgery in a direct and indirect manner. In the first case, the weight of the lower pole was divided by overall spleen weight; in the second case, pole weight was divided by the ideal weight of the spleen obtained by linear regression analysis. The results of the two calculations were compared. Macro- and microscopic examinations of the pole were performed. RESULTS: In group 1, no significant difference in mean percent pole weight was observed between the direct and indirect method. In group 2, mean percent pole weight obtained by indirect calculation on day 80 was higher than in group 1 (p<0.001). In group 2, mean length, width and thickness of the pole remnant increased from the first to the 80th day (p<0.05). Histological analysis showed preserved tissue architecture and features compatible with cell hyperplasia in group 2. CONCLUSION: The lower pole splenic remnant presented statistically significant growth up to postoperative day 80 after subtotal splenectomy, even after ligature of the major spleen vessels. Light microscopy revealed changes compatible with cell hyperplasia.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Growth of the lower spleen pole remaining after subtotal splenectomy in rats

et al. 2008

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“…As alterações no metabolismo lipídico têm sido relatadas após esplenectomia total tanto em seres humanos 6 quanto em animais de experimentação [7][8][9][10][11] e podem causar aterosclerose 7 . Por essas razões têm sido valorizadas as operações conservadoras sobre o baço que compreendem as oclusões vasculares [12][13][14][15][16] , esplenorrafias 17 , esplenectomias parciais [18][19][20] e os auto-implantes 21,22 . Dessas, a mais recentemente descrita, em cães, foi a esplenectomia subtotal com preservação do pólo inferior, mesmo com a ligadura dos vasos esplênicos principais 23 .…”

Section: Introductionunclassified

O pólo inferior do baço de ratos e a oxigenoterapia hiperbárica

Paulo¹,

Paulo²,

Ferrari³

et al. 2008

Rev. Assoc. Med. Bras.

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RESUMOOBJETIVO. Estudar aspectos funcionais e morfológicos do pólo inferior do baço de ratos tratados ou não com oxigênio hiperbárico no pós-operatório. MÉTODOS. Foram operados 79 ratos, Wistar, pesando 248,7g ± 27 , distribuídos em dois grupos: A -Simulação (n= 40), B -pólo inferior (n=39), e divididos em dois subgrupos: 11 e 70 dias. Cada subgrupo foi subdividido em não tratado com oxigênio hiperbárico (nt) (A11nt-n=10, B11 nt-n= 13, A70nt-n= 10, B70nt-n= 9) e tratado (t); A11t-n=10, B11t-n=9, A70 t-n= 10, B70t-n= 8). Foram dosados os lípides e imunoglobulinas e contadas as plaquetas e os corpúsculos de HowellJolly no pré e no pós-operatório. O baço e o pólo inferior foram retirados para histologia. RESULTADOS. Houve aumento do colesterol total (p=0,002), VLDL-colesterol e triglicérides (p= 0,003), da 013) INTRODUÇÃOA esplenectomia total pode provocar complicações no transoperatório e no pós-operatório 1 . Dessas, a mais preocupante é a infecção fulminante que foi relatada por King e Schumaker Jr 2 e foi recentemente revista 3 . As complicações infecciosas, após a retirada do baço, foram também observadas em animais de experimentação 4,5 . As alterações no metabolismo lipídico têm sido relatadas após esplenectomia total tanto em seres humanos 6 quanto em animais de experimentação 7-11 e podem causar aterosclerose 7 . Por essas razões têm sido valorizadas as operações conservadoras sobre o baço que compreendem as oclusões vasculares [12][13][14][15][16] , esplenorrafias 17 , esplenectomias parciais 18-20 e os auto-implantes 21,22 . Dessas, a mais recentemente descrita, em cães, foi a esplenectomia subtotal com preservação do pólo inferior, mesmo com a ligadura dos vasos esplênicos principais 23 . Nessa operação, observou-se aumento significante do colesterol e da fração LDL no pós-operatório mediato, e sugeriu-se a possibilidade de isquemia do pólo inferior remanescente. Por esse motivo, seria interessante utilizar um método para combater a isquemia e, possivelmente, melhorar a função do pólo inferior, no pós-operatório mediato.A oxigenoterapia hiperbárica (OHB) é um método terapêutico no qual o indivíduo é submetido à inalação intermitente de oxigênio (O 2 ) a 100%, a uma pressão maior que a atmosférica, no interior de uma câmara hiperbárica 24 . Essa modalidade terapêutica tem sido utilizada no tratamento de várias doenças. No Brasil, a Resolução CFM 1457/95 do Conselho Federal de Medicina regulamentou seu uso nas seguintes indicações: embolia gasosa, embolia traumática pelo ar, envenenamento por monóxido de carbono, gangrena gasosa clostridiana, doença de Fournier, isquemias agudas traumáticas, queimaduras térmicas ou elé-tricas, lesões por irradiação, osteomielite, retalhos ou enxertos comprometidos ou de risco. No nível experimental demonstrou-se que a OHB tem efeito angiogênico 25,26 , estimula a formação de circulação colateral 27 , aumenta o conteúdo arterial de oxigênio e a elasticidade dos glóbulos vermelhos 28 , acelera a cicatrização do baço após a ligadura simultânea da artéria e veia esplênicas...

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“…The investigation of the inferior pole of the spleen of dogs and rats [4][5][6] submitted to ESPTI showed alterations in the viability of the remaining tissue, in the immediate postoperative period. Rats submitted to this procedure, followed by hyperbaric oxygenation (HBO) at 100% pure oxygen, in conventional microscopy, showed larger lymphatic follicles, increase of the amount of cells and vessels and more lymphocytic cell proliferation in the inferior pole of the spleen 7,8 .…”

Section: Introductionmentioning

confidence: 98%

Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation

et al. 2013

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PURPOSE:To analyze PCNA immunoexpression on the inferior pole of the spleen of splenectomized rats submitted to hyperbaric oxygenation (HBO). METHODS:Were analyzed fragments of the inferior pole of the spleen of 20 male Wistar rats submitted to splenectomy with preservation of the inferior pole. The rats were divided in two groups: group A (n=10) without HBO and group B (n=10) submitted to HBO at 2, 5 atmospheres per 120 minutes, twice a day for three days and once a day for seven days. The groups were then subdivided in four subgroups: A15 (n=5), with euthanasia on the 15 th day; A45 (n=5), with euthanasia on the 45 th day; B15 (n=5) with euthanasia on the 15 th day and B45 with euthanasia on the 45 th day. Respectively on these days, fragments of the inferior pole of the spleen of all animals were collected and analyzed with the immunohistochemistry technique in order to evaluate PCNA expression. RESULTS:There was an expressive increase in PCNA immunoreactivity in the group B. The 45 day postoperative period resulted in a higher level of positivity than the 15 day postoperative period (p<0.01). CONCLUSION:The quantitative analysis of proliferating cell nuclear antigen positive suggests that hyperbaric oxygenation increases cellular proliferation, contributing to splenic regeneration.

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Esplenectomia subtotal, em cães, com preservação do pólo inferior(PI) suprido por vasos do ligamento gastroesplênico

Cited by 14 publications

References 12 publications

Growth of the lower spleen pole remaining after subtotal splenectomy in rats

Growth of the lower spleen pole remaining after subtotal splenectomy in rats

O pólo inferior do baço de ratos e a oxigenoterapia hiperbárica

Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation

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